RESUMO
Glutamatergic neurons in the cerebral cortex are derived from embryonic neural stem cells known as radial glial progenitors (RGPs). Early RGPs, present at the onset of cortical neurogenesis, are classically thought to produce columnar clones of glutamatergic neurons spanning the cortical layers. Recently, however, it has been reported that a subset of early RGPs may undergo early commitment to upper layer neuron fates, thus bypassing genesis of deep layer neurons. However, the latter mode of early RGP differentiation was not confirmed in some other studies, and remains controversial. To further investigate the clonal output from early RGPs, we employed genetic lineage tracing driven by Sox9, a transcription factor gene that is expressed in all early RGPs. We found that early RGPs produced columnar clones spanning all cortical layers, with no evidence of significant laminar fate restriction. These data support the classic progressive restriction model of cortical neurogenesis, and suggest that early RGPs do not undergo early commitment to only upper or lower layer fates.
Assuntos
Diferenciação Celular/fisiologia , Neocórtex/embriologia , Células-Tronco Neurais/citologia , Neurogênese/fisiologia , Neurônios/citologia , Animais , Glutamina/metabolismo , CamundongosRESUMO
T-box transcription factors play key roles in the regulation of developmental processes such as cell differentiation and migration. Mammals have 17 T-box genes, of which several regulate brain development. The Tbr1 subfamily of T-box genes is particularly important in development of the cerebral cortex, olfactory bulbs (OBs), and cerebellum. This subfamily is comprised of Tbr1, Tbr2 (also known as Eomes), and Tbx21. In developing cerebral cortex, Tbr2 and Tbr1 are expressed during successive stages of differentiation in the pyramidal neuron lineage, from Tbr2+ intermediate progenitors to Tbr1+ postmitotic glutamatergic neurons. At each stage, Tbr2 and Tbr1 regulate laminar and regional identity of cortical projection neurons, cell migration, and axon guidance. In the OB, Tbr1 subfamily genes regulate neurogenesis of mitral and tufted cells, and glutamatergic juxtaglomerular interneurons. Tbr2 is also prominent in the development of retinal ganglion cells in nonimage-forming pathways. Other regions that require Tbr2 or Tbr1 in development or adulthood include the cerebellum and adult dentate gyrus. In humans, de novo mutations in TBR1 are important causes of sporadic autism and intellectual disability. Further studies of T-box transcription factors will enhance our understanding of neurodevelopmental disorders and inform approaches to new therapies.
Assuntos
Encéfalo/metabolismo , Neurogênese/genética , Neurônios/metabolismo , Proteínas com Domínio T/genética , Sequência de Aminoácidos , Animais , Humanos , Modelos Biológicos , Mutação/genética , Proteínas com Domínio T/química , Proteínas com Domínio T/metabolismoRESUMO
Cortical development involves the structuring of network features by genetically programmed molecular signaling pathways. Additionally, spontaneous ion channel activity refines neuronal connections. We examine Ca(2+) fluctuations in the first postnatal week of normal mouse neocortex and that expressing knockout of the transcription factor T-brain-1 (Tbr1): a signaling molecule in cortical patterning and differentiation of excitatory neurons. In cortex, glutamatergic neurons express Tbr1 just before the onset of population electrical activity that is accompanied by intracellular Ca(2+) increases. It is known that glutamatergic cells are disordered with Tbr1 KO such that normal laying of the cortex, with newer born cells residing in superficial layers, does not occur. However, the fate of cortical interneurons is not well studied, nor is the ability of Tbr1 deficient cortex to express normal physiological activity. Using fluorescent proteins targeted to interneurons, we find that cortical interneurons are also disordered in the Tbr1 knockout. Using Ca(2+) imaging we find that population activity in mutant cortex occurs at normal frequencies with similar sensitivity to GABAA receptor blockade as in nonmutant cortex. Finally, using multichannel fluorescence imaging of Ca(2+) indicator dye and interneurons labeled with red fluorescent protein, we identify an additional Ca(2+) signal in interneurons distinct from population activity and with different pharmacological sensitivities. Our results show the population activity described here is a robust property of the developing network that continues in the absence of an important signaling molecule, Tbr1, and that cortical interneurons generate distinct forms of activity that may serve different developmental functions. © 2015 Wiley Periodicals, Inc. Develop Neurobiol 76: 705-720, 2016.
Assuntos
Sinalização do Cálcio/fisiologia , Proteínas de Ligação a DNA/fisiologia , Interneurônios/fisiologia , Neocórtex/fisiologia , Animais , Proteínas de Ligação a DNA/genética , Feminino , Masculino , Camundongos , Camundongos Knockout , Neocórtex/embriologia , Neocórtex/crescimento & desenvolvimento , Imagem Óptica , Proteínas com Domínio TRESUMO
The mammalian neocortex is subdivided into cytoarchitectural areas with distinct connectivity, gene expression and neural functions. Areal identity is initially specified by rostrocaudal and mediolateral gene expression gradients in neuroepithelial and radial glial progenitors (the 'protomap'). On further differentiation, distinct sets of gene expression gradients arise in intermediate progenitors and postmitotic neurons, and are necessary to implement areal specification. However, it is still unknown whether postmitotic gene expression gradients can determine areal identity independently of protomap gradients. Here we show, by cell type-restricted genetic loss- and gain-of-function, that high levels of postmitotic COUP-TFI (Nr2f1) expression are necessary and sufficient for the development of sensory (caudal) areal identity. Our data indicate a crucial role for postmitotic patterning genes in areal specification and reveal an unexpected plasticity in this process, which may account for complex and evolutionarily novel structures characteristic of the mammalian neocortex.
Assuntos
Mitose , Neocórtex/crescimento & desenvolvimento , Células Receptoras Sensoriais/citologia , Animais , Fator I de Transcrição COUP/genética , Fator I de Transcrição COUP/metabolismo , Feminino , Regulação da Expressão Gênica no Desenvolvimento , Masculino , Camundongos , Camundongos Knockout , Neocórtex/citologia , Neocórtex/metabolismo , Células Receptoras Sensoriais/metabolismoRESUMO
Mammalian corticogenesis occurs through a complex process that includes neurogenesis, in which neural progenitor cells proliferate, differentiate, and migrate. It has been reported recently that neurogenesis occurs in the subventricular zone (SVZ), a region previously thought to be the primary site of gliogenesis. It has been recognized that in the SVZ, intermediate progenitor cells, derived from radial glial cells that are multipotent neural stem cells, produce only neurons. However, the molecular mechanisms underlying the regulation of neural stem cells and intermediate progenitor cells as well as their contribution to overall corticogenesis remain unknown. The docking protein FRS2alpha is a major mediator of signaling by means of FGFs and neurotrophins. FRS2alpha mediates many of its pleiotropic cellular responses by recruiting the adaptor protein Grb2 and the protein tyrosine phosphatase Shp2 upon ligand stimulation. Here, we report that targeted disruption of Shp2-binding sites in FRS2alpha leads to severe impairment in cerebral cortex development in mutant mice. The defect in corticogenesis appears to be due at least in part to abnormalities in intermediate progenitor cells. Genetic evidence is provided that FRS2alpha plays critical roles in the maintenance of intermediate progenitor cells and in neurogenesis in the cerebral cortex. Moreover, FGF2-responsive neurospheres, which are cell aggregates derived from neural stem/progenitor cells (NSPCs), from FRS2alpha mutant mice were smaller than those of WT mice. However, mutant NSPCs were able to self-renew, demonstrating that Shp2-binding sites on FRS2alpha play an important role in NSPC proliferation but are dispensable for NSPC self-renewing capacity after FGF2 stimulation.
Assuntos
Córtex Cerebral/crescimento & desenvolvimento , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Proteínas de Membrana/metabolismo , Neurônios/citologia , Proteínas Tirosina Fosfatases/metabolismo , Receptor Tipo 2 de Fator de Crescimento de Fibroblastos/fisiologia , Células-Tronco/citologia , Animais , Sítios de Ligação , Proliferação de Células , Células Cultivadas , Córtex Cerebral/citologia , Embrião de Mamíferos , Imuno-Histoquímica , Peptídeos e Proteínas de Sinalização Intracelular/fisiologia , Proteínas de Membrana/fisiologia , Camundongos , Camundongos Mutantes , Proteína Tirosina Fosfatase não Receptora Tipo 11 , Proteínas Tirosina Fosfatases/fisiologiaRESUMO
In the developing brain, neuronal differentiation is associated with permanent exit from the mitotic cycle. This raises the possibility that neuronal differentiation may suppress proliferative activity, even in neoplastic cells. As a first step towards understanding the relation between neuronal differentiation and mitotic cycling in brain tumours, we studied the expression of NeuN (a neuronal marker) and Ki-67 (a mitotic marker) by double-labelling immuno-fluorescence in 16 brain tumours with neuronal differentiation. The tumours included a series of 11 central neurocytomas, and five single cases of other tumour types. In the central neurocytomas, NeuN(+) cells had a 15-fold lower Ki-67 labelling index, on average, than did NeuN(-) cells (P < 0.01). In the other tumours (one extraventricular neurocytoma, one desmoplastic medulloblastoma, one olfactory neuroblastoma, one ganglioglioma and one anaplastic ganglioglioma), the Ki-67 labelling index was always at least fourfold lower in NeuN(+) cells than in NeuN(-) cells. These results indicate that neuronal differentiation is associated with a substantial decrease of proliferative activity in neoplastic cells of central neurocytomas, and suggest that the same may be true across diverse types of brain tumours. However, tumours with extensive neuronal differentiation may nevertheless have a high overall Ki-67 labelling index, if the mitotic activity of NeuN(-) cells is high. The correlation between NeuN expression and reduced mitotic activity in neurocytoma cells is consistent with the hypothesis that neuronal differentiation suppresses proliferation, but further studies will be necessary to determine causality and investigate underlying mechanisms.
Assuntos
Neoplasias Encefálicas/metabolismo , Índice Mitótico , Proteínas do Tecido Nervoso/biossíntese , Neurocitoma/metabolismo , Neurônios/citologia , Adolescente , Adulto , Diferenciação Celular/fisiologia , Criança , Pré-Escolar , Feminino , Imunofluorescência , Humanos , Processamento de Imagem Assistida por Computador , Antígeno Ki-67/metabolismo , Masculino , Microscopia Confocal , Pessoa de Meia-Idade , Neurônios/metabolismoRESUMO
During development, interneurons migrate to precise positions in the cortex by tangential and radial migration. The objectives of this study were to characterize the net radial migrations of interneurons during the first postnatal week, and to investigate the role of reelin signaling in regulating those migrations. To observe radial migrations, we compared the laminar positions of interneurons (immunoreactive for GABA or Dlx) in mouse neocortex on postnatal days (P) 0.5 and P7.5. In addition, we used bromodeoxyuridine birthdating to reveal the migrations of different interneuron cohorts. To study the effects of reelin deficiency, experiments were performed in reeler mutant mice. In normal P0.5 cortex, interneurons were most abundant in the marginal zone and layer 5. By P7.5, interneurons were least abundant in the marginal zone, and were distributed more evenly in the cortical plate. This change was attributed mainly to inward migration of middle- to late-born interneurons (produced on embryonic days (E) 13.5 to E16.5) from the marginal zone to layers 2-5. During the same interval, late-born projection neurons (non-immunoreactive for GABA or Dlx) migrated mainly outward, from the intermediate zone to upper cortical layers. In reeler cortex, middle- and late-born interneurons migrated from the superplate on P0.5, to the deep cortical plate on P7.5. Late-born projection neurons in reeler migrated in the opposite direction, from the intermediate zone to the deep cortical plate. We conclude that many middle- and late-born interneurons migrate radially inward, from the marginal zone (or superplate) to the cortical plate, during the first postnatal week in normal and reeler mice. We propose that within the cortical plate, interneuron laminar positions may be determined in part by interactions with projection neurons born on the same day in neurogenesis.
Assuntos
Movimento Celular/genética , Córtex Cerebral/anormalidades , Córtex Cerebral/crescimento & desenvolvimento , Interneurônios/metabolismo , Camundongos Mutantes Neurológicos/embriologia , Camundongos Mutantes Neurológicos/crescimento & desenvolvimento , Animais , Animais Recém-Nascidos , Apoptose/genética , Padronização Corporal/genética , Bromodesoxiuridina , Moléculas de Adesão Celular Neuronais/deficiência , Moléculas de Adesão Celular Neuronais/genética , Diferenciação Celular/genética , Divisão Celular/genética , Linhagem da Célula , Córtex Cerebral/patologia , Proteínas da Matriz Extracelular/deficiência , Proteínas da Matriz Extracelular/genética , Feto , Proteínas de Homeodomínio/metabolismo , Imuno-Histoquímica , Interneurônios/patologia , Camundongos , Camundongos Mutantes Neurológicos/genética , Proteínas do Tecido Nervoso , Malformações do Sistema Nervoso/genética , Malformações do Sistema Nervoso/patologia , Malformações do Sistema Nervoso/fisiopatologia , Inibição Neural/genética , Vias Neurais/anormalidades , Vias Neurais/crescimento & desenvolvimento , Vias Neurais/patologia , Proteína Reelina , Serina Endopeptidases , Fatores de Transcrição/metabolismo , Ácido gama-Aminobutírico/metabolismoRESUMO
The dorsal cyst is poorly understood, although it is commonly encountered in holoprosencephaly. We endeavor to establish the role of diencephalic malformations in the formation of the dorsal cyst and speculate on the developmental factors responsible. We reviewed the imaging of 70 patients with holoprosencephaly (MRI of 50 and high-quality CT of 20). The presence or absence of a dorsal cyst, thalamic noncleavage and abnormal thalamic orientation were assessed for statistical association, using Fisher's Exact Test and logistical regression. The presence of a dorsal cyst correlated strongly with the presence of noncleavage of the thalamus (P = 0.0007) and with its degree (P < 0.00005). There was a trend toward an association between abnormalities in the orientation of the thalamus and the dorsal cyst, but this was not statistically significant (P = 0.07). We speculate that the unseparated thalamus physically blocks egress of cerebrospinal fluid from the third ventricle, resulting in expansion of the posterodorsal portion of the ventricle to form the cyst.
Assuntos
Cistos/congênito , Holoprosencefalia/diagnóstico , Tálamo/anormalidades , Adolescente , Ventrículos Cerebrais/fisiopatologia , Criança , Pré-Escolar , Cistos/complicações , Holoprosencefalia/fisiopatologia , Humanos , Hidrocefalia/líquido cefalorraquidiano , Lactente , Recém-Nascido , Modelos Logísticos , Imageamento por Ressonância Magnética , Tomografia Computadorizada por Raios XRESUMO
During corticogenesis, early-born neurons of the preplate and layer 6 are important for guiding subsequent neuronal migrations and axonal projections. Tbr1 is a putative transcription factor that is highly expressed in glutamatergic early-born cortical neurons. In Tbr1-deficient mice, these early-born neurons had molecular and functional defects. Cajal-Retzius cells expressed decreased levels of Reelin, resulting in a reeler-like cortical migration disorder. Impaired subplate differentiation was associated with ectopic projection of thalamocortical fibers into the basal telencephalon. Layer 6 defects contributed to errors in the thalamocortical, corticothalamic, and callosal projections. These results show that Tbr1 is a common genetic determinant for the differentiation of early-born glutamatergic neocortical neurons and provide insights into the functions of these neurons as regulators of cortical development.
Assuntos
Moléculas de Adesão Celular Neuronais/metabolismo , Proteínas de Ligação a DNA/fisiologia , Proteínas da Matriz Extracelular/metabolismo , Regulação da Expressão Gênica no Desenvolvimento/fisiologia , Camadas Germinativas/metabolismo , Neocórtex/embriologia , Animais , Morte Celular , Movimento Celular/fisiologia , Proteínas de Ligação a DNA/genética , Óperon Lac/fisiologia , Camundongos , Camundongos Mutantes , Camundongos Transgênicos , Mutação , Neocórtex/anormalidades , Neocórtex/crescimento & desenvolvimento , Proteínas do Tecido Nervoso , Vias Neurais/fisiologia , Neurônios/metabolismo , Proteína Reelina , Serina Endopeptidases , Transmissão Sináptica , Proteínas com Domínio TRESUMO
Animal studies have shown that connections between the retina, lateral geniculate nucleus (LGN), and visual cortex begin to develop prenatally. To study the development of these connections in humans, regions of fixed brain from fetuses of 20-22 gestational weeks (GW) were injected with the fluorescent tracer DiI. Placement of DiI in the optic nerve or tract labeled retinogeniculate projections. In the LGN, these projections were already segregated into eye-specific layers by 20 GW. Retinogeniculate segregation thus preceded cellular lamination of the LGN, which did not commence until 22 GW. Thalamocortical axons, labeled from DiI injections into the optic radiations, densely innervated the subplate, but did not significantly innervate the cortical plate. This pattern was consistent with observations of a "waiting period" in animals, when thalamocortical axons synapse in the subplate for days or weeks before entering the cortical plate. Cortical efferent neurons (labeled retrogradely from the optic radiations) were located in the subplate and deep layers of the cortical plate. In summary, human visual connections are partially formed by mid-gestation, and undergo further refinement during and after this period. The program for prenatal development of visual pathways appears remarkably similar between humans and other primates.
Assuntos
Feto/fisiologia , Vias Visuais/embriologia , Carbocianinas , Vias Eferentes/embriologia , Desenvolvimento Embrionário e Fetal , Corantes Fluorescentes , Corpos Geniculados/citologia , Corpos Geniculados/embriologia , Idade Gestacional , Humanos , Retina/embriologia , Transmissão Sináptica , Córtex Visual/embriologiaRESUMO
Huntington's disease (HD) is a dominant disorder characterized by premature and progressive neurodegeneration. In order to generate an accurate model of the disease, we introduced an HD-like mutation (an extended stretch of 72-80 CAG repeats) into the endogenous mouse Hdh gene. Analysis of the mutation in vivo reveals significant levels of germline instability, with expansions, contractions and sex-of-origin effects in evidence. Mice expressing full-length mutant protein display abnormal social behaviour in the absence of acute neurodegeneration. Given that psychiatric changes, including irritability and aggression, are common findings in HD patients, our data are consistent with the hypothesis that some clinical features of HD may be caused by pathological processes that precede gross neuronal cell death. This implies that effective treatment of HD may require an understanding and amelioration of these dysfunctional processes, rather than simply preventing the premature death of neurons in the brain. These mice should facilitate the investigation of the molecular mechanisms that underpin the pathway from genotype to phenotype in HD.
Assuntos
Mutação em Linhagem Germinativa/genética , Doença de Huntington/genética , Transtornos Mentais/genética , Camundongos Mutantes/genética , Proteínas do Tecido Nervoso/genética , Proteínas Nucleares/genética , Animais , Comportamento Animal , Encéfalo/patologia , Feminino , Humanos , Proteína Huntingtina , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos , Repetições de TrinucleotídeosRESUMO
In this report, the authors discuss the case of a patient with a mixed cerebrovascular malformation in which an arteriovenous malformation (AVM) was associated with a capillary telangiectasia. Recent reports have contained reviews of various subsets of mixed malformations. To the authors' knowledge, however, this is the first report of a mixed vascular malformation with both arterial and capillary components. The patient underwent complete resection of the AVM after presenting with a clinical hemorrhage. She required a second operation to resect the capillary telangiectasia after new symptoms developed several months following the first procedure. The authors conclude that a mixed AVM-capillary telangiectasia is a rare but distinct entity.
Assuntos
Malformações Arteriovenosas Intracranianas/complicações , Telangiectasia/complicações , Adulto , Encéfalo/patologia , Capilares , Angiografia Cerebral , Feminino , Humanos , Malformações Arteriovenosas Intracranianas/diagnóstico , Malformações Arteriovenosas Intracranianas/cirurgia , Imageamento por Ressonância Magnética , Reoperação , Telangiectasia/diagnóstico , Telangiectasia/cirurgiaRESUMO
Autopsy examination of an infant with the Pena-Shokeir phenotype revealed bilateral opercular polymicrogyria associated with neuronal loss and ferrugination in the basal ganglia, thalamus, brainstem, and spinal anterior horns. Bilateral opercular polymicrogyria previously has been linked to the developmental form of Foix-Chavany-Marie syndrome, or faciopharyngoglossomasticatory diplegia. In the Pena-Shokeir phenotype, bilateral opercular polymicrogyria may contribute to deficits in swallowing and facial movements. The pattern of brain and spinal cord injury in this case supports previous suggestions that the Pena-Shokeir phenotype (and certain other forms of arthrogryposis multiplex congenita) may be caused by hypoxic-ischemic injury to the developing central nervous system.
Assuntos
Anormalidades Múltiplas/patologia , Encéfalo/anormalidades , Dominância Cerebral/fisiologia , Anormalidades Múltiplas/genética , Encéfalo/patologia , Feminino , Hipóxia Fetal/patologia , Humanos , Recém-Nascido , Degeneração Neural/fisiologia , Neurônios/patologia , Fenótipo , Fatores de Risco , Medula Espinal/patologiaRESUMO
Little is known about the timing or sequence of genesis of connections between different areas of the developing human cerebral cortex. It has been shown that connections between areas V1 and V2 of the visual isocortex are established at about 37 weeks of gestation (Burkhalter [1993] Cerebr. Cortex 3:476-487), suggesting that cortico-cortical connections appear late in the 40-week human gestational period. However, there are indications from other studies that connections between subdivisions of the hippocampal formation may be established much earlier, by about 20 weeks of human gestation. To investigate this possibility, the lipophilic bidirectional tracer 1,1' dioctadecyl-3,3,3',3-tetramethylindocarbocyanine perchlorate (DiI) was used to study connections between the entorhinal cortex, hippocampus, and temporal lobe neocortex in paraformaldehyde-fixed postmortem fetal tissue. The DiI transport revealed robust reciprocal connections between the entorhinal cortex, hippocampus, and subiculum, which were consistently present at 19 weeks of gestation (the earliest age studied), and which were anatomically similar to those in adult primates. Specifically, projections to the hippocampus and subiculum originated from neurons in the entorhinal cortex (EC) layers 2 and 3, whereas reciprocal projections to the EC originated from pyramidal neurons in the cornu ammonis region CA1 and the subiculum. In contrast, the perforant pathway projection from EC to the dentate gyrus, and all connections with the neocortex, reached only rudimentary stages of development by 22 weeks of gestation (the latest age studied). These findings suggest that hippocampal pathways develop prior to isocortical pathways, and that reciprocal entorhinal-hippocampal projections may be among the first cortico-cortical connections to be established in the human brain.
Assuntos
Encéfalo/crescimento & desenvolvimento , Córtex Entorrinal/anatomia & histologia , Hipocampo/anatomia & histologia , Vias Neurais/anatomia & histologia , Córtex Entorrinal/ultraestrutura , Hipocampo/ultraestrutura , Humanos , Microscopia EletrônicaRESUMO
To examine brain patterns of metabolic and functional activity, the distribution of cytochrome oxidase, a mitochondrial enzyme marker for neuronal functional activity, was mapped throughout the rat brain. Mapping was done qualitatively by enzyme histochemistry of brain sections cut in three planes (coronal, sagittal and horizontal), and quantitatively by optical densitometry of stained sections and by biochemical assays of brain tissue homogenates. Activity of the enzyme was distributed in characteristic patterns and amounts that differed among various neural pathways, brain nuclei, cerebral cortical areas and layers, and neuron types. Gray matter essentially always had higher enzyme activity than did white matter, by a factor of eight- to 12-fold. Among different neural pathways, cytochrome oxidase activity was relatively high in special sensory, somatosensory and motor systems, and was relatively low in associative, limbic, autonomic and visceral regulatory systems (though exceptional areas were present). Among 11 different neuron types, nearly a two-fold range of histochemical staining intensities was observed, with the darkest staining in neurons of the mesencephalic trigeminal nucleus. The observed patterns of cytochrome oxidase activity were mostly similar to the patterns of 2-deoxyglucose uptake seen previously [Schwartz W. J. and Sharp F. R. (1978) J. comp. Neurol. 177, 335-360; Sokoloff L. et al. (1977) J. Neurochem. 28, 897-916] in conscious, "resting" animals, though some differences were found. For example, whereas 2-deoxyglucose uptake was about three-fold higher in gray matter than in white matter [Sokoloff L. et al. (1977) J. Neurochem. 28, 897-916], cytochrome oxidase activity was about eight- to 12-fold higher. This and other discrepancies probably reflect basic technical differences between these two methods. Compared to 2-deoxyglucose, cytochrome oxidase is more specific for oxidative rather than glycolytic metabolism, and more reflective of overall neuronal functional activity occurring over longer time periods lasting hours to weeks, rather than minutes. The anatomical resolution of cytochrome oxidase histochemistry is also finer than that of 2-deoxyglucose autoradiography, extending to the electron microscopic level. The metabolic map of cytochrome oxidase activity reveals patterns of normal brain function, and may be useful as a baseline for comparison in studies of brain disease, development, ageing and plasticity.
Assuntos
Encéfalo/enzimologia , Complexo IV da Cadeia de Transporte de Elétrons/metabolismo , Animais , Encéfalo/anatomia & histologia , Encéfalo/citologia , Mapeamento Encefálico , Densitometria , Feminino , Histocitoquímica , Masculino , Neurônios/enzimologia , Ratos , Ratos Sprague-DawleyRESUMO
We have developed a method to accurately and reproducibly determine the total activity of cytochrome oxidase (CO) in rat brain tissue homogenates. Previously, accurate measurements have been difficult to obtain because detergents, which are needed to disrupt membranes and unmask CO, also inhibit the enzyme by solubilizing certain phospholipids required for rapid turnover. We compared various methods of sample preparation, and found that maximal CO activity in homogenates could be obtained using specific concentrations of detergents. The range of optimal detergent concentrations was relatively narrow, as CO activity fell sharply with small deviations from the optimum. Of 5 detergents tested, deoxycholate stimulated CO maximally over the widest range of concentrations. In deoxycholate-treated homogenate samples, the calculated CO turnover number was about 480 s-1, indicating that overall enzyme activity was maximal or near maximal, and therefore that the total content of CO was probably detected. This method was reproducible with large or small samples (e.g., < 1 mg tissue), and should be applicable to studies of neural tissue in general.
Assuntos
Encéfalo/enzimologia , Complexo IV da Cadeia de Transporte de Elétrons/análise , Animais , Ácido Desoxicólico/metabolismo , Complexo IV da Cadeia de Transporte de Elétrons/antagonistas & inibidores , Metabolismo Energético/fisiologia , Feminino , Histocitoquímica , Técnicas In Vitro , Masculino , Ratos , Ratos Sprague-Dawley , Ratos Wistar , Retina/enzimologiaRESUMO
Mitochondrial respiratory complexes such as cytochrome oxidase (CO) contain both mitochondrial- and nuclear-encoded subunits. To determine whether mitochondrial and nuclear gene expression are regulated proportionately in neurons, we analyzed CO subunit mRNA and mitochondrial DNA (mtDNA) levels by in situ hybridization and grain counting in the visual system of normal and monocular TTX-treated monkeys. We compared the regulation of these molecules with the regulation of CO activity and CO protein, analyzed by histochemistry and immunohistochemistry, respectively. In normal animals, CO activity was in general related more closely to mtDNA and CO subunit I (COI) (mitochondrial-encoded) mRNA levels than to COIV or COVIII (nuclear-encoded) mRNA levels. For example, puffs (also known as blobs) of high CO activity in striate cortex were enriched in mtDNA and COI mRNA, but not COIV or COVIII mRNA. In 3-7 d TTX-treated animals, proportionate decreases in CO activity and CO protein were observed in specific visual centers; these changes were accompanied by disproportionate decreases in COI, COIV, and COVIII mRNA levels. After 7 d of TTX, COI mRNA fell by 49 +/- 3% (mean +/- SEM) in LGN neurons, while COIV and COVIII mRNAs fell by only 18 +/- 3% and 29 +/- 3%, respectively. In comparison, CO activity decreased by 23 +/- 2%, and mtDNA by 26 +/- 4%. Qualitative observations in striate cortex also indicated that COI mRNA changed more than COIV mRNA, COVIII mRNA, mtDNA, or CO activity. Our results suggest that the local distribution of CO within neurons, and acute regulatory changes in CO activity occurring over periods of days are controlled mainly by regulation of the mitochondrial genes that encode the catalytic subunits of the enzyme.
Assuntos
Núcleo Celular/fisiologia , Complexo IV da Cadeia de Transporte de Elétrons/genética , Expressão Gênica , Mitocôndrias/fisiologia , Neurônios/fisiologia , Animais , Corpo Estriado/metabolismo , Complexo IV da Cadeia de Transporte de Elétrons/química , Complexo IV da Cadeia de Transporte de Elétrons/metabolismo , Processamento Eletrônico de Dados , Corpos Geniculados/metabolismo , Imuno-Histoquímica , Hibridização In Situ , RNA Mensageiro/metabolismo , Prata , Tetrodotoxina/farmacologiaRESUMO
Cytochrome-oxidase (CO) histochemistry has revealed important functional subdivisions, modules, and processing streams in the macaque visual cortex. The present study is aimed at analyzing the development and characteristics of CO patterns in the human visual cortex by means of histochemistry and immunohistochemistry. At 26 weeks of gestation, both the ventricular and subventricular zones had low levels of CO, while the cortical plate had moderate levels of CO. At birth, supragranular CO-rich zones (puffs) were not clearly organized, indicating that the development of puffs in the neonatal striate cortex lags behind that of the macaque monkey, whose puffs appear weeks before birth. Puffs were more clearly discernible in human cortex at postnatal day 24, and became well organized by the fourth postnatal month. Layer IVc alpha in the neonate exhibited a higher level of activity and amount of CO than the central portion of IVc beta, which contained a dense aggregate of small neurons. The base of IVc beta, however, was often as CO reactive as IVc alpha. In contrast, the majority of specimens available to us from the fourth postnatal month and from adults with no known neurological diseases had significantly greater CO reactivity in layer IVc beta than in IVc alpha. Layer VI was moderately reactive for CO throughout development. In V2, stripes with globular zones of high CO activity were sporadically present at birth, suggesting that their development may parallel or precede that of puffs in V1. These stripes with CO-rich globular zones became more prominent in the adult and radiated orthogonally from the V1/V2 border. They were not, however, clearly organized into alternating thick and thin stripes as they are in the squirrel monkey. Visual cortical areas beyond V2 exhibited high CO activity mainly in layers III and IV and moderate levels in VI, suggesting that sites associated with cortico-cortical pathways may be metabolically most active.
Assuntos
Complexo IV da Cadeia de Transporte de Elétrons/metabolismo , Córtex Visual/enzimologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Densitometria , Feminino , Feto , Idade Gestacional , Histocitoquímica , Humanos , Técnicas Imunoenzimáticas , Lactente , Recém-Nascido , Masculino , Pessoa de Meia-Idade , Córtex Visual/embriologia , Córtex Visual/crescimento & desenvolvimentoRESUMO
The entorhinal cortex (EC) is a medial temporal lobe area involved in memory consolidation. Results from previous studies suggest that the upper layers of the EC may be organized into anatomical-neurochemical modules associated with pathways through the neuron clusters in layers II and III. To study metabolic patterns in the EC and to look for correlates of the proposed modules, we examined the distribution of cytochrome oxidase (CO) in the human, monkey, and rat EC. CO is a mitochondrial enzyme that has been used to study modules in other cortical areas. In all three species, the neuron clusters in layers II-III were darkly CO-reactive, whereas most of the neuropil between clusters was lightly or moderately CO-reactive. However, some neuropil regions directly adjacent to the neuron clusters were also darkly CO-reactive, especially in the human; these neuropil areas included portions of layers I and II. In tangential sections through layers I-II, the areas of dark staining formed a consistent pattern, comprised of partially interconnected islands and stripes associated with the neuron clusters. In the EC from one human hemisphere, approximately 200-250 CO-reactive layer II islands were present. EC layers other than I-III also showed characteristic CO staining intensities, but no evidence of modularity. Our results indicate that CO staining labels distinct compartments related to the neuron clusters in the upper EC layers. We propose that these compartments may represent modules for cortical processing, analogous to the CO-labeled modules in some other areas of cortex.