High Arctic channel incision modulated by climate change and the emergence of polygonal ground.

Stream networks in Arctic and high-elevation regions underlain by frozen ground (i.e., permafrost) are expanding and developing in response to accelerating global warming, and intensifying summertime climate variability. The underlying processes governing landscape dissection in these environments are varied, complex and challenging to unravel due to air-temperature-regulated feedbacks and shifts to new erosional regimes as climate change progresses. Here we use multiple sources of environmental information and physical models to reconstruct and understand a 60-year history of landscape-scale channelization and evolution of the Muskox Valley, Axel Heiberg Island. A time series of air photographs indicates that freeze-thaw-related polygon fields can form rapidly, over decadal time scales. Supporting numerical simulations show that the presence of polygons can control how surface runoff is routed through the landscape, exerting a basic control on channelization, which is sensitive to the timing, duration and magnitude of hydrograph events, as well as seasonal air temperature trends. These results collectively highlight that the occurrence and dynamics of polygon fields modulate channel network establishment in permafrost-rich settings undergoing changes related to a warming climate.

Sequence specific detection of DNA using nicking endonuclease signal amplification (NESA).

We have developed a new method for identifying specific single- or double-stranded DNA sequences called nicking endonuclease signal amplification (NESA). A probe and target DNA anneal to create a restriction site that is recognized by a strand-specific endonuclease that cleaves the probe into two pieces leaving the target DNA intact. The target DNA can then act as a template for fresh probe and the process of hybridization, cleavage and dissociation repeats. Laser-induced fluorescence coupled with capillary electrophoresis was used to measure the probe cleavage products. The reaction is rapid; full cleavage of probe occurs within one minute under ideal conditions. The reaction is specific since it requires complete complementarity between the oligonucleotide and the template at the restriction site and sufficient complementarity overall to allow hybridization. We show that both Bacillus subtilis and B. anthracis genomic DNA can be detected and specifically differentiated from DNA of other Bacillus species. When combined with multiple displacement amplification, detection of a single copy target from less than 30 cfu is possible. This method should be applicable whenever there is a requirement to detect a specific DNA sequence. Other applications include SNP analysis and genotyping. The reaction is inherently simple to multiplex and is amenable to automation.