Adaptations to the stressful combination of serpentine soils and Mediterranean climate drive plant functional groups and trait richness.

Introduction: Plant functional traits (FTs) are important for understanding plant ecological strategies (e.g., drought avoidance), especially in the nutrient-poor soils of serpentine ecosystems. In the Mediterranean areas, such ecosystems are characterized by climatic factors (e.g., summer drought) that exert a filtering effect. Material and Methods: In our study, we analyzed 24 species with varying serpentine affinity, from strictly serpentine plants to generalist plants, from two ultramafic shrublands in southern Spain, considering four FTs: plant height (H), leaf area (LA), specific leaf area (SLA), and stem specific density (SSD). Additionally, we also identified the species' dominant strategies to avoid drought and those strategies' relationship to serpentine affinity. We used principal component analysis to identify combinations of FTs, and cluster analysis to define Functional Groups (FGs). Results and Discussion: We defined eight FGs, which suggests that such Mediterranean serpentine shrublands are composed of species with wide-ranging of FTs. Indicator traits explained 67-72% of the variability based on four strategies: (1) lower H than in other Mediterranean ecosystems; (2) middling SSD; (3) low LA; and (4) low SLA due to thick and/or dense leaves, which contribute to long leaf survival, nutrient retention, and protection from desiccation and herbivory. Generalist plants had higher SLA than obligate serpentine plants, whereas the obligate serpentine plants showed more drought avoidance mechanisms than the generalists. Although most plant species inhabiting Mediterranean serpentine ecosystems have shown similar ecological adaptations in response to the Mediterranean environment, our results suggest that serpentine obligate plant species could present greater resilience to climate change. Given greater number and more pronounced drought avoidance mechanisms in these species compared with generalists, and the high number of FGs identified, the serpentine plants have shown adaptation to severe drought.

The resource availability hypothesis (RAH) and cross-cultural patterns: which one explains West African Cochlospermum species' uses in Benin?

BACKGROUND: Cochlospermum tinctorium and C. planchonii are two wide edible plants of sub-Saharan countries, e.g., Benin, widely used as food, medicine, dye, handicraft, etc. Unfortunately, the uncontrolled harvest of their rootstocks exposes them to local extension risk. To enhance knowledge on the determinants of their uses in Benin, this study aimed to (i) assess the use forms, use values, abundance and perceived spatiotemporal dynamics, (ii) determine how does local abundance or cultural patterns affect the use of Cochlospermum species, and (iii) assess local harvesting modes and conservation management practices. METHODS: In total, 756 Dialog Partners through 27 ethnic groups were questioned countrywide using semi-structured interviews. Questions refer to local taxonomy, specific uses, organs sought, harvesting modes and local conservation strategies; afterward, local abundance of each species was assessed. Ethnobotanical indicators were analyzed through citation frequencies to obtain quantitative data. Comparison tests and statistical analyses were performed using R program. RESULTS: C. tinctorium and C. planchonii are locally well known and involved into 83 specific uses, grouped into ten categories of which medicinal use was the main. The use values of C. planchonii (0.10 ± 0.19) and C. tinctorium (0.23 ± 0.20) varied significantly between the ethnic groups, and only C. tinctorium showed index of commercially value above 75% for some ethnic groups. The current abundance, about 84 and 97 tufts.ha-1, respectively, for C. planchonii and C. tinctorium was perceived with a decline of 81.09% (C. tinctorium) and 73.7% (C. planchonii) of informants. Moreover, the Spearman's correlation and Kruskal-Wallis tests performed revealed that the use values of C. tinctorium and C. planchonii were significantly correlated on the one hand with their local abundance and on the other hand with the investigated ethnic groups. About 42.3% of women produced the powder as principal activity, while more than 57% produced it mainly at the end of farm work. However, the conservation management was practiced by very few informants and consisted of partial harvesting of rootstocks (41.8%, only in southern Benin), and fallowing of harvesting areas (3.97%, only in northern Benin). CONCLUSION: Facing the declining abundance and increasing market demand for rootstock powder of Cochlospermum species, existing local conservation strategies should be promoted and the domestication process should be initiated for sustainable management of these important wild edible plants before these important resources disappear completely in the wild.

Variation of Secondary Metabolite Contents and Activities against Bovine Diarrheal Pathogens among Zygophyllaceae Species in Benin and Implications for Conservation.

Balanites aegyptiaca is a wild plant species largely used in folk medicine and a priority fruit tree in West Africa. In Benin, its overexploitation for ethnoveterinary uses could lead to its rarity or extinction in the long term. In this study, we evaluate the possibilities of its substitution by other Zygophyllaceae species. This study was based on optimal defense theory, which distinguished 2 categories of plants: K-strategist species and r-strategist species. Phytochemical screening was carried out based on aqueous extracts of the leafy stems of B. aegyptiaca and Guaiacum officinale (K-strategist species) and Tribulus terrestris and Kallstroemia pubescens (r-strategist species) for the identification of chemical compounds. The phenolic compounds were quantified by quercetin and vanillin methods. The extracts were tested against 5 bacterial strains responsible for severe diarrhea in bovines. Our results indicated the presence of many phytochemicals, such as alkaloids, flavonoids, saponosides, and tannins. The diversity in secondary metabolites is higher for r-strategist than K-strategist species. The contents of total polyphenols ranged from 4.82 ± 0.05 to 41.84 mg GAE/g of extract. The flavonoid contents varied from 30.64 ± 0.35 to 57.11 ± 0.13 mg QE/g of extract and those of the tannins from 0.04 ± 0.00 to 0.06 ± 0.01 mg PE/mL. The sensitivity of the bacterial strains showed a significant dependence on the extracts. Of the species, K. pubescens showed a bactericidal activity on the majority of strains tested and thus could be a potential substitute for B. aegyptiaca in the treatment of infectious diarrhea.

Metal accumulation by Alyssum serpyllifolium subsp. malacitanum Rivas Goday (Brassicaceae) across different petrographic entities in South-Iberian ultramafic massifs: plant-soil relationships and prospects for phytomining.

NOVELTY STATEMENT: To date, studies of hyperaccumulation in plant tissues on ultramafic rocks have not considered the great diversity of petrographic entities in the world's outcrops. One of them is the one that we studied in Spain with more than eight petrographic entities and different soils. Our hypothesis is that the different chemical compositions of the soils in ultramafic rocks significantly affect the hyperaccumulation of metals by specialized plants, which may have consequences for phytomining. For this purpose, individuals, populations, and different soils have been tested and the results have been subjected to the corresponding statistical tests. The obtained knowledge reflects the different behavior of the studied plant not only for the Ni: the obtained results for Sr and for Ba revealed interesting results for the hyperaccumulation in Alyssum of both metals.

Smart Tree: An Architectural, Greening and ICT Multidisciplinary Approach to Smart Campus Environments.

At present, climate change, pollution, and uncontrolled urbanism threaten not only natural ecosystems, but also the urban environment. Approaches to mitigate these challenges and able to provide an alternative for the use of the space are deemed to be multidisciplinary, combining architecture, vegetation integration, circular economy and information and communications technologies (ICT). University campuses are a key scenario to evaluate such solutions as their student and research community is intrinsically willing to support these experiences and provide a wide knowledge on the fields necessary for their design and implementation. However, the creation of areas combining usability and sustainability is commonly lacking a multidisciplinary approach combining all these different perspectives. Hence, the present work aims to overcome this limitation by the development of a novel integrated approach for campus spaces for co-working and leisure, namely a "Smart Tree", where novel architecture, furniture design, flora integration, environmental sensoring and communications join together. To this end, a survey of the literature is provided, covering related approaches as well as general principles behind them. From this, the general requirements and constraints for the development of the Smart Tree area are identified, establishing the main interactions between the architecture, greening and ICT perspectives. Such requirements guide the proposed system design and implementation, whose impact on the environment is analyzed. Finally, the research challenges and lessons learned for their development are identified in order to support future works.

Variations in Leaf Traits Modulate Plant Vegetative and Reproductive Phenological Sequencing Across Arid Mediterranean Shrublands.

Structural and nutrient traits of a leaf are important for understanding plant ecological strategies (e.g., drought avoidance). We studied the specific leaf area (SLA), leaf carbon content (LCC), leaf nitrogen content (LNC), leaf phosphorous content (LPC), and the phenophase sequence index (PSI) in 126 Mediterranean perennial species from predesert (SMS) and semiarid (SaMS) to subalpine (SAS), alpine cushion (AcS), and oro-Mediterranean (AjS) shrublands, which represent eight functional groups (evergreen and deciduous trees, evergreen large and half shrubs, deciduous large and half shrubs, succulents and perennial herbs). We analyzed the variation and relationships between leaf traits and PSI among shrublands, functional groups, and within species with drought-avoidance mechanisms. SLA variation of 20-60% could be ascribed to differences between functional groups and only 38-48% to different shrublands increasing from the predesert to the alpine. Alpine species display low PSI and N:P and high SLA, LNC, LPC, LCC, and C:N. On the contrary, predesert and semiarid showed high PSI and low SLA. SLA mediates the vegetative and reproductive phenological plant sequencing, high SLA is often associated with the overlapping in growth and reproductive phenophases with a seasonal reduction of vegetative growth, whereas low SLA is associated with vegetative and reproductive sequencing and a seasonal extension of vegetative growth. Species with drought-avoidance mechanisms (e.g., semideciduous species) contribute to an increase in the mean values of the SLA and LNC because these species show similar leaf and phenological patterns as the deciduous (high SLA and LNC and low PSI). The N:P indicates that only the alpine shrublands could present P limitations. The positive correlations between SLA and LPC and LNC and LPC (leaf economic spectrum) and the negative correlation between SLA and C:N were consistently maintained in the studied arid Mediterranean shrublands.