RESUMO
A patient presented with decreased flexion and triggering following a zone IIc flexor digitorum profundus repair 7 years previously. Chalky-white deposits, confirmed histologically as gout, were present at the repair site.
Assuntos
Traumatismos dos Dedos/cirurgia , Gota/etiologia , Complicações Pós-Operatórias , Traumatismos dos Tendões/cirurgia , Tenossinovite/etiologia , Adulto , Gota/patologia , Gota/cirurgia , Humanos , Masculino , Tenossinovite/patologia , Tenossinovite/cirurgiaRESUMO
OBJECTIVE: Numerous methods for isolation of human chondrocytes are reported in the literature, most based on isolation from animal cartilage. Normal human articular cartilage (NHAC) poses particular problems for isolating chondrocytes when compared to animal or other types of human cartilage: a hardy matrix, combined with few and friable chondrocytes makes isolation difficult. Our objective was to develop an efficient method of isolating chondrocytes from NHAC without jeopardising the viability of these cells. DESIGN: In this study we demonstrate that lowering the enzymatic digestion temperature to 27 degrees C increases cell yield and chondrocyte viability. We then optimised this low temperature isolation of chondrocytes from NHAC by comparing the relative efficacies of trypsin and protease and hyaluronidase in combination with different types of collagenase (I, II and XI) at releasing chondrocytes from their surrounding cartilaginous matrix. Enzymes were tested at different concentrations and for differing times. Outcome measures included determining the amount of cartilage digested, the number of viable chondrocytes isolated per gram of cartilage and cell adherence rates. CONCLUSIONS: From these set of experiments, the method that maximised cell yield without jeopardising cell viability proved to be a two stage process: pre-digestion step using trypsin for 15 min; followed by overnight digestion with a combination of two types of collagenase (types I and II) and at a lower temperature of 27 degrees C. This has resulted in an efficient and robust method of releasing chondrocytes from cartilage, without jeopardising the viability of these cells.
Assuntos
Cartilagem Articular/citologia , Condrócitos/citologia , Idoso , Idoso de 80 Anos ou mais , Cartilagem Articular/enzimologia , Adesão Celular/fisiologia , Separação Celular/métodos , Sobrevivência Celular/fisiologia , Células Cultivadas , Condrócitos/enzimologia , Temperatura Baixa , Colagenases/metabolismo , Humanos , Hialuronoglucosaminidase/metabolismo , Peptídeo Hidrolases/metabolismo , Tripsina/metabolismoRESUMO
We present a case of squamous cell carcinoma arising in a naevus sebaceous of Jadassohn (NSJ) in a 15-year-old girl. Both these events are rare, and this is the first reported case of a squamous cell carcinoma developing in NSJ in a child. The previous literature on sebaceous naevi and malignancies arising thereof is reviewed and discussed. Early excision is recommended in any cases of nodular change in sebaceous naevi in all age groups.
Assuntos
Carcinoma de Células Escamosas/cirurgia , Neoplasias de Cabeça e Pescoço/cirurgia , Nevo/cirurgia , Couro Cabeludo , Neoplasias Cutâneas/cirurgia , Adolescente , Carcinoma de Células Escamosas/complicações , Carcinoma de Células Escamosas/patologia , Feminino , Neoplasias de Cabeça e Pescoço/complicações , Neoplasias de Cabeça e Pescoço/patologia , Humanos , Nevo/complicações , Nevo/patologia , Neoplasias das Glândulas Sebáceas/complicações , Neoplasias das Glândulas Sebáceas/patologia , Neoplasias das Glândulas Sebáceas/cirurgia , Neoplasias Cutâneas/complicações , Neoplasias Cutâneas/patologia , Resultado do TratamentoRESUMO
Vancomycin-resistant enterococci (VRE) are multi-resistant micro-organisms that have emerged as important nosocomial pathogens during the last decade. Emergence of this organism has been blamed mainly on the increased and inappropriate use of antibiotics, in particular, the cephalosporins and the glycopeptide, vancomycin. Burns patients are highly vulnerable to acquiring VRE infections, being both debilitated and immunocompromised, and often receiving antibiotics that further diminish their intrinsic microbial flora. We report on two patients with large burn injuries who acquired vancomycin-resistant enterococcal septicaemia during their in-patient stay. Both patients were successfully treated using the antibiotic, linezolid. Linezolid is the first in a new class of antibiotics known as the oxazolidinones whose mode of action inhibits early bacterial protein synthesis. Linezolid has a spectrum of activity against Gram-positive micro-organisms including methicillin-resistant Staphylococcus aureus (MRSA) and VRE, and can provide a useful treatment alternative to the glycopeptides.