RESUMO
Aflatoxins, particularly aflatoxin B1 (AFB1) have been recognized as one of the most potent chemical carcinogen. In Egypt, HCV is prevalent. The progressive nature of HCV-related liver diseases was found to be influenced by other factors. In this paper, the role of aflatoxin contamination in the onset of liver cancer in HCV-infected patients was studied. The quantitative identification of the possible aflatoxins contamination in six urban and eleven rural areas using high performance liquid chromatography technique, revealed that corn, wheat, pea nut, lupine "termis", white rice, cowpea "lobiya", fava bean and brown rice showed the prevalence of AFB1 to be 64.7%, 53%, 53%, 47%, 47%, 41%, 29.4% & 29.4% respectively. A positive correlation was found between aflatoxin and positive HCV-PCR together with liver disease progression to G3S3, the indicative of hepatocellular carcinoma. Such correlation was not fully understood, but the oncogene amplification caused by HCV-infection may be aggravated by the consumption of aflatoxin contaminated raw food materials or their products.
Assuntos
Aflatoxinas/efeitos adversos , Carcinoma Hepatocelular/etiologia , Contaminação de Alimentos , Hepatite C Crônica/complicações , Neoplasias Hepáticas/etiologia , Carcinoma Hepatocelular/epidemiologia , Cromatografia Líquida de Alta Pressão , Progressão da Doença , Microbiologia de Alimentos , Humanos , Neoplasias Hepáticas/epidemiologiaAssuntos
Antineoplásicos Fitogênicos/farmacologia , Glicosídeos Cardíacos/farmacologia , Plantas Medicinais/análise , Alanina Transaminase/sangue , Fosfatase Alcalina/metabolismo , Animais , Aspartato Aminotransferases/sangue , Cálcio/sangue , Sobrevivência Celular/efeitos dos fármacos , Humanos , Células KB , Masculino , Ratos , Ratos Endogâmicos , Arábia Saudita , Ácido Úrico/sangue , gama-Glutamiltransferase/metabolismoAssuntos
Óleos Voláteis/isolamento & purificação , Plantas Medicinais/análise , Álcoois/isolamento & purificação , Aldeídos/isolamento & purificação , Fenômenos Químicos , Química , Cromatografia Gasosa-Espectrometria de Massas/métodos , Cetonas/isolamento & purificação , Fenóis/isolamento & purificação , Arábia Saudita , Espectrofotometria InfravermelhoRESUMO
Two colorimetric micromethods are described for the determination of atropine-hyoscyamine and hyoscine (scopolamine), using p-dimethylaminobenzaldehyde and citric acid-acetic anhydride as the color reagents. These methods are sensitive to 60-1200 and 10-360 mug alkaloid/10 ml. The colorimetric methods were also successfully applied after a preliminary thin layer chromatographic separation of the alkaloids. A gas-liquid chromatographic procedure was also developed, which yielded comparable results with the colorimetric methods.
Assuntos
Atropina/análise , Colorimetria/métodos , Escopolamina/análise , Cromatografia Gasosa , Cromatografia Líquida de Alta Pressão , Cromatografia em Camada Fina , Microquímica , Plantas Medicinais/análiseRESUMO
Two colorimetric methods are described for the estimation of strychnine and brucine in nux vomica. The first is a modification of the Karawya and Ghourab method for the determination of strychnine, in which the sensitivity of the color is increased by changing certain conditions of the method. The second was developed for the determination of brucine and is based on measuring the intensity of the violet color produced by treating brucine with nitric acid and methanolic stannous chloride. In the presence of large amounts of strychnine, brucine is isolated prior to colorimetric analysis by a quantitative thin layer chromatographic technique.