RESUMO
Histochemical studies on the activities of alpha-GPDH, LDH, SDH and MDH in liver have been carried out during the different phases of tail regeneration in the lizard, Hemidactylus flaviviridis. Changes in the metabolic activities of the liver during regeneration indicate that during the initial phases of regeneration (namely, wound healing and blastema formation) the energetics of the hepatic tissue are anaerobically oriented, but later (i.e. during the growth phase) the TCA cycle appears to be predominant.
Assuntos
Fígado/enzimologia , Lagartos/fisiologia , Oxirredutases/metabolismo , Regeneração , Animais , Glicerolfosfato Desidrogenase/metabolismo , Histocitoquímica , L-Lactato Desidrogenase/metabolismo , Lagartos/metabolismo , Malato Desidrogenase/metabolismo , Succinato Desidrogenase/metabolismo , Cauda/fisiologiaAssuntos
Aminoácidos/metabolismo , Lagartos/fisiologia , Regeneração , Cauda/fisiologia , Animais , Cauda/metabolismoRESUMO
Histochemical demonstration of beta-glucuronidase was carried out in the normal and regenerating tail of the house lizard, Hemidactylus flaviviridis. Greater enzyme activity was particularly discernible in the stratum germinativum of the skin of the normal and fully regenerated tail. During regeneration most of the cellular elements of the blastema and the differentiating regenerate exhibited enzyme activity. Distribution of the enzyme beta-glucuronidase is correlated with the high degree of cellular proliferation. The role of the enzyme in the catabolism of mucopolysaccharides is also implicated during the wound-healing phase. In the full-grown regenerate the intensity and distribution of the enzyme activity were found to be the same as those observed in the normal tail.
