The cephalothoracic sucker of sea lice (Crustacea: Copepoda: Caligidae): The functional importance of cuticular membrane ultrastructure.

Sea lice adhere to the body surface of host fish with a cephalothoracic sucker. Caligus adheres to this substrate using legs 2 and 3, and the action of cephalothoracic muscles. Lunules, small, paired, anterior sucker-like structures, have a vital function in the initial step of adhering and contain a unique endocuticule containing elements that may behave like active matter and serve as the actuating mechanism. Cuticular membranes bordering the cephalothorax have a unique endocuticule with an undulating dorsal surface and a smooth ventral surface. A high-speed camera revealed that this undulation likely facilitates rapid automatic application of the sucker to the substrate. The cuticular membranes on the posterior margin of the first exopodal segment of leg 2 have a specialized endocuticle with tubules each surrounded by fine fibers. This reinforcement helps them to generate a posteriorly-directed jet of water. Opening-closing of these membranes is controlled by postero-anterior motion of the distal exopodal segments of leg 2. The outer cuticular membrane of leg 3 is simple, presumably effected by powerful extrinsic muscles. The consistency of sucker morphology within Caligus implies a highly stereotyped attachment behavior that is effective across a remarkable variety of fishes.

Discovery of the fish host of the 'planktonic' caligid Caligus undulatus Shen & Li, 1959 (Crustacea: Copepoda: Siphonostomatoida).

The siphonostomatoid copepod Caligus undulatus Shen & Li, 1959 has been widely reported from plankton samples obtained from neritic and oceanic waters off coasts of the Indo-West Pacific and Atlantic Oceans. Until now, its fish host has remained unknown. This copepod belongs to an intriguing group of congeners that, despite being part of a chiefly parasitic group, are consistently found as zooplankters. Quite unexpectedly, in October 2019, a fish host of C. undulatus was discovered in the Seto Inland Sea, Japan-namely, the Japanese sardinella Sardinella zunasi (Bleeker, 1854). Both juvenile (chalimus) and adult individuals of this caligid were observed as parasites of the fish host. The discovery suggests that the species has an alternative life cycle as previously proposed for other purportedly 'planktonic' congeners and might frequently switch hosts during the adult stage. Thus, the C. undulatus group is newly proposed as a species group in the genus, in which five species are known as planktonic. Some hypotheses on the modified life cycle of caligids also briefly discussed.