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Mycol Res ; 108(Pt 1): 45-52, 2004 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-15035504

RESUMO

Phenotypic and genotypic variability of 18 endophytic Guignardia strains from different host plants (Anacardiaceae: Anacardium giganteum, Myracrodruon urundeuva, Spondias mombin; Apocynaceae: Aspidosperma polyneuron; Ericaceae: Rhododendron sp.; Fabaceae: Bowdichia nitida; Leguminosae: Cassia occidentalis; Rutaceae: Citrus aurantium) growing in different sites in Brazil was assessed by means of morphometric measurements and inter-single-sequence-repeat-anchored polymerase chain reaction (ISSR-PCR) amplifications of the DNA. Morphology of conidia and ascospores and growth rates of the Brazilian isolates corresponded well with those of G. mangiferae. Multiple correspondence analysis (MCA) of the ISSR-PCR data yielded three groups of strains, which did, however, not correspond either to the host or to the geographic origin. The same individual tree hosted genotypically different strains indicating multiple infections. Phylogeny based on ITS1-5.8S-ITS2 sequence analyses confirmed conspecificity of the Brazilian isolates with G. mangiferae (syn. G. endophyllicola).


Assuntos
Ascomicetos/genética , DNA Espaçador Ribossômico/química , Plantas/microbiologia , Reação em Cadeia da Polimerase/métodos , Sequências Repetitivas de Ácido Nucleico , Ascomicetos/citologia , Ascomicetos/isolamento & purificação , Filogenia , Clima Tropical
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