Pharmacokinetic studies of a three-component complex that repurposes the front line antibiotic isoniazid against Mycobacterium tuberculosis.

The frontline tuberculosis (Tb) antibiotic isoniazid has been repurposed using a three component complex aimed at increasing the delivery efficiency and adding new avenues to its mechanism of action. This study focuses on pharmacokinetic studies of the isoniazid-sucrose-copper (II)-PEG-3350 complex. The assays include the Plasma Protein Binding Assay (85.8%), Caco-2 Permeability Assay (B→APapp, 0.13 × 10-6 cm/s), Cytochrome P450 Inhibition Assay (i.e. CYP2B6, IC50 = 7.26 µM), In vitro microsomal Stability Assay (t1/2 NADPH-Dependent > 240 min), and HepG2 Cytotoxicity (no toxicity). The National Cancer Institute's 60 cell line panel is used to measure activity against cancer cells. The percent growth values averaged over all 60 cell lines indicates the complex has no anti-cancer activity, which also suggests a lack of general toxicity. It also provides data for the complexes specificity against Mycobacterium tuberculosis.

The Role of Trust in Low-Income Mothers' Intimate Unions*

Recent scholarship concerning low rates of marriage among low-income mothers emphasizes generalized gender distrust as a major impediment in forming sustainable intimate unions. Guided by symbolic interaction theory and longitudinal ethnographic data on 256 low-income mothers from the Three-City Study, we argue that generalized gender distrust may not be as influential in shaping mothers' unions as some researchers suggest. Grounded theory analysis revealed that 96% of the mothers voiced a general distrust of men, yet that distrust did not deter them from involvement in intimate unions. Rather, the pivotal ways mothers enacted trust in their partners were demonstrated by 4 emergent forms of interpersonal trust that we labeled as suspended, compartmentalized, misplaced, and integrated. Implications for future research are discussed.