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1.
BAG, J. basic appl. genet. (Online) ; 33(1): 83-88, Oct. 2022. graf
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1420288

RESUMO

ABSTRACT The subfamily Bambusoideae comprises three monophyletic tribes, Arundinarieae, Bambuseae and Olyreae. Here we report the gametic number and the chromosomal meiotic behavior of two species belonging to the herbaceous tribe Olyreae, Olyra latifolia and Olyrahumilis. Accessions were collected in Misiones, at Northeastern Argentina. We report a new gametic number for O. humilis, n=18, and we confirmed n=11 for O. latifolia. Chromosomal features, like the basic and gametic chromosome number, are important in understanding the evolution of the Poaceae family, especially in delimiting clades and elucidate inter andintra-clades relationships, and therefore it is necessary to continue producing this type of data.


RESUMEN La subfamilia Bambusoideae comprende tres tribus monofiléticas, Arundinarieae, Bambuseae y Olyreae. Aquí reportamos el número gamético y el comportamiento meiótico de los cromosomas de dos especies pertenecientes a la tribu Olyreae de bambúes herbáceos, Olyra latifolia y Olyra humilis. Las introducciones se recolectaron en la provincia de Misiones, en el noreste argentino. Reportamos un nuevo número gamético para O. humilis, n=18, y confirmamos n=11 para O. latifolia. Los números cromosómicos básicos y gaméticos son importantes para comprender la evolución de la familia Poaceae, especialmente para delimitar sus clados y las relaciones existentes entre ellos, por lo que es necesario continuar produciendo este tipo de datos.

2.
BAG, J. basic appl. genet. (Online) ; 33(1): 89-95, Oct. 2022. graf
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1420289

RESUMO

ABSTRACT Zephyranthes citrina is an ornamental American bulbous plant used as an ornamental garden crop for the aesthetic qualities of its yellow perigonium. The objective of this work was to characterize the species by classical chromosome staining and fluorochrome banding. A sporophytic chromosome number of 2n=8x=48 chromosomes was observed, being the karyotypic formula 20 m + 26 sm + 2 st. Satellites were detected in the short arm of metacentric chromosomes 8, 9, 11 and 12, which colocalized with constitutive heterochromatin CMA+/DAPI-/0 bands. The karyotype comprised chromosome pairs with terminal constitutive heterochromatin bands that included satellites and heteromorphic clusters indicating that it is an allooctoploid. These results will be used as a tool for monitoring genetic improvement, in interspecific crosses and its progenies and in biotechnological procedures by in vitro culture.


RESUMEN Zephyranhtes citrina es una planta bulbosa americana, ornamental, utilizada en jardines por las cualidades estéticas de su perigonio amarillo. El objetivo de este trabajo fue caracterizar citogenéticamente la especie con tinción clásica convencional y bandeo cromosómico. Se observó un número cromosómico esporofítico de 2n=8x=48, siendo la fórmula cariotípica 20 m + 26 sm + 2st. Se detectaron satélites en el brazo corto de los cromosomas metacéntricos 8, 9, 11 y 12, que co-localizaron con bandas de heterocromatina constitutiva CMA+/DAPI-. El cariotipo comprendió pares de cromosomas con bandas de heterocromatina constitutivas terminales que incluyeron satélites y grupos heteromórficos que indican que es un alooctoploide. Estos resultados serán usados como herramientas en el monitoreo del mejoramiento genético, en análisis de cruzamientos interespecíficos y progenies y en procedimientos biotecnológicos de cultivo in vitro.

3.
J Plant Res ; 129(4): 697-710, 2016 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-26965283

RESUMO

It is generally accepted that polyploids have downsized basic genomes rather than additive values with respect to their related diploids. Changes in genome size have been reported in correlation with several biological characteristics. About 75 % of around 350 species recognized for Paspalum (Poaceae) are polyploid and most polyploids are apomictic. Multiploid species are common with most of them bearing sexual diploid and apomictic tetraploid or other ploidy levels. DNA content in the embryo and the endosperm was measured by flow cytometry in a seed-by-seed analysis of 47 species including 77 different entities. The relative DNA content of the embryo informed the genome size of the accession while the embryo:endosperm ratio of DNA content revealed its reproductive mode. The genome sizes (2C-value) varied from 0.5 to 6.5 pg and for 29 species were measured for the first time. Flow cytometry provided new information on the reproductive mode for 12 species and one botanical variety and supplied new data for 10 species concerning cytotypes reported for the first time. There was no significant difference between the mean basic genome sizes (1Cx-values) of 32 sexual and 45 apomictic entities. Seventeen entities were diploid and 60 were polyploids with different degrees. There were no clear patterns of changes in 1Cx-values due to polyploidy or reproductive systems, and the existing variations are in concordance with subgeneric taxonomical grouping.


Assuntos
DNA de Plantas/genética , Diploide , Paspalum/classificação , Paspalum/fisiologia , Poliploidia , Cromossomos de Plantas/genética , Citometria de Fluxo , Paspalum/genética , Reprodução/genética , Especificidade da Espécie
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