Effects of distraction task on driving: a functional magnetic resonance imaging study.

This study investigated neuronal activation differences under two conditions: driving only and distracted driving. Driving and distraction tasks were performed using a Magnetic Resonance (MR)-compatible driving simulator with a driving wheel and pedal. The experiment consisted of three blocks, and each block had both a Rest phase (1 min) and a Driving phase (2 min). During the Rest phase, drivers were instructed to simply look at the stop screen without performing any driving tasks. During the Driving phase, each driver was required to drive at 110 km/h under two conditions: driving only and driving while performing additional distraction tasks. The results show that the precuneus, inferior parietal lobule, supramarginal gyrus, middle frontal gyrus, cuneus, and declive are less activated in distracted driving than in driving only. These regions are responsible for spatial perception, spatial attention, visual processing and motor control. However, the cingulate gyrus and sub-lobar regions (lentiform nucleus and caudate), which are responsible for error monitoring and control of unnecessary movement, show increased activation during distracted driving compared with driving only.

Comparative transcriptomic analysis to identify differentially expressed genes in fat tissue of adult Berkshire and Jeju Native Pig using RNA-seq.

Pork is a major source of animal protein for humans. The subcutaneous, intermuscular and the intramuscular fat are the factors responsible for meat quality. RNA-seq is rapidly adopted for the profiling of the transcriptomes in the studies related to gene regulation. The discovery of differentially expressed genes (DEGs) between adult animals of Jeju Native Pig (JNP) and Berkshire breeds are of particular interest for the current study. RNA-seq was used to investigate the transcriptome profiling in the fat tissue. Sequence reads were obtained from Ilumina HiSeq2000 and mapped to the pig genome using Tophat2. Total 153 DEGs were identified and 71 among the annotated genes, have BLAST matches in the non- redundant database. Metabolic, immune response and protein binding are enriched pathways in the fat tissue. In our study, biological adhesion, cellular, developmental and multicellular organismal processes in fat were up-regulated in JNP as compare to Berkshire. Multicellular organismal process, developmental process, embryonic morphogenesis and skeletal system development were the most significantly enriched terms in fat of JNP and Berkshire breeds (p = 1.17E-04, 0.044, 3.47E-04 and 4.48E-04 respectively). COL10A1, COL11A2, PDK4 and PNPLA3 genes responsible for skeletal system morphogenesis and body growth were down regulated in JNP. This study is the first statistical analysis for the detection of DEGs from RNA-seq data generated from fat tissue sample. This analysis can be used as stepping stone to understand the difference in the genetic mechanisms that might influence the identification of novel transcripts, sequence polymorphisms, isoforms and noncoding RNAs.

Comparative transcriptomic analysis by RNA-seq to discern differential expression of genes in liver and muscle tissues of adult Berkshire and Jeju Native Pig.

RNA-seq is being rapidly adopted for the profiling of the transcriptomes in different areas of biology, especially in the studies related to gene regulation. The discovery of differentially expressed genes (DEGs) between adult animals of Jeju Native Pig (JNP) and Berkshire breeds of Sus scrofa, is of particular interest for the current study. For the better understanding of the gene expression profiles of the liver and longissimus dorsi muscle, DEGs were identified via RNA-seq. Sequence reads were obtained from Illumina HiSeq2000 and mapped to the pig reference genome (Sscrofa10.2) using Tophat2. We identified 169 and 39 DEGs in the liver and muscle of JNP respectively, by comparison with Berkshire breed. Out of all identified genes, 41 genes in the liver and 9 genes in the muscle have given significant expression. Gene ontology (GO) terms of developmental process and KEGG pathway analysis showed that metabolic, immune response and protein binding were commonly enriched pathways in the two tissues. Further the heat map analysis by ArrayStar has shown the different levels of expression in JNP with respect to the Berkshire breed. The validation through real time PCR and western blotting also confirmed the differential expression of genes in both breeds. Genes pertaining to metabolic process and inflammatory and immune system are more enriched in Berkshire breed. This comparative transcriptome analysis of two tissues suggests a subset of novel marker genes which expressed differently between the JNP and Berkshire.

Response time of visual matching task and heart rate in children with Attention Deficit Hyperactivity Disorder (ADHD).

The purpose of this study was to investigate the relation between response time of visual matching task and heart rate (HR) in children with Attention Deficit Hyperactivity Disorder (ADHD). Thirty boys who were diagnosed with ADHD and are under treatment participated in the study. The experiment consisted of three phases, a total of 5 min with rest phase, control phase, and visual matching task phase. HR was measured during each phase. The HR in visual matching task phase increased, compared to that in rest phase. There was a negative correlation between response time of visual matching task and magnitude of the HR in the visual matching task phase. In other words, as HR increased, response time of the visual matching task decreased. This means that increasing in HR increased the supply of oxygen by fast circulation of blood for cognitive processing and this induced the improvement of cognitive ability in the ADHD children. This means that increasing HR increased the supply of oxygen by fast circulation of blood for cognitive processing and this induced the improvement of cognitive ability in the ADHD children. The result of this study supports previous studies that the administration of high oxygen concentration can positively affect the cognitive performance of the ADHD children. The results of the present and previous studies also may provide scientific evidence that can be used for treating patients with cognitive problems such as ADHD.

Development of a simple pressure and heat stimulator for intra- and interdigit functional magnetic resonance imaging.

For this study, we developed a simple pressure and heat stimulator that can quantitatively control pressure and provide heat stimulation to intra- and interdigit areas. The developed stimulator consists of a control unit, drive units, and tactors. The control unit controls the stimulation parameters, such as stimulation types, intensity, time, and channel, and transmits a created signal of stimulation to the drive units. The drive units operate pressure and heat tactors in response to commands from the control unit. The pressure and heat tactors can display various stimulation intensities quantitatively, apply stimulation continuously, and adjust the stimulation areas. Additionally, they can easily be attached to and detached from the digits. The developed pressure and heat stimulator is small in total size, easy to install, and inexpensive to manufacture. The new stimulator operated stably in a magnetic resonance imaging (MRI) environment without affecting the obtained images. A preliminary functional magnetic resonance imaging (fMRI) experiment confirmed that differences in activation of somatosensory areas were induced from the pressure and heat stimulation. The developed pressure and heat stimulator is expected to be utilized for future intra- and interdigit fMRI studies on pressure and heat stimulation.

Changes in simple visual matching task performance and physiological signals in intellectually and developmentally disabled people due to administration of highly concentrated oxygen.

BACKGROUND: This study attempted to identify the effect of administration of highly concentrated oxygen on simple visual matching task performance, blood oxygen saturation [SpO2 (%)], and heart rate [HR (bpm)] of intellectually and developmentally disabled people. METHODS: Nineteen intellectually and developmentally disabled people (mean age 30.6 ± 5.7 years) participated in an experiment consisting of a simple visual matching task performed under 21% and 92% oxygen. SpO2 and HR were measured under both oxygen conditions. RESULTS: When 92% oxygen was supplied, the response time decreased, SpO2 increased, and HR decreased compared to the vales obtained using 21% oxygen. The response time decreased for subjects with a high SpO2 and HR during the simple visual matching task phase. CONCLUSION: This result supports the hypothesis that administration of highly concentrated oxygen can positively affect the cognitive performance of intellectually and developmentally disabled people.

Driving performance changes of middle-aged experienced taxi drivers due to distraction tasks during unexpected situations.

This study investigated the effects of distraction taskssuch as sending a text message with a cellphone and searching navigation with car navigation system-on the driving performance of 29 highly experienced taxi drivers in their 50s. All participants were instructed to drive using a driving simulator for 2 min. while maintaining a constant distance from the vehicle in front and a constant speed. Participants drove without any distractions for the first minute. For an additional minute, they performed Driving Only or performed a task while driving (Driving + Sending Text Message or Driving + Searching Navigation). An unexpected situation, in which the participant had to stop abruptly due to a sudden stop of the preceding vehicle, occurred during this period. Driving performance during the unexpected situation was evaluated by car control variables, medial-lateral coefficient of variation and brake time, and by motion variables such as the jerk-cost function. Compared to Driving Only, jerk-cost function, medial-lateral coefficient of variation, and brake time increased during Driving + Sending Text Message or Driving + Searching Navigation.

The effects of disruption in attention on driving performance patterns: analysis of jerk-cost function and vehicle control data.

This study analyzes the effects of attention disruption factors, such as sending text messages (STM) and performing searching navigation (SN) on driving performance patterns while actively driving, centering on motion signals. To this end, it analyzes not only data on control of the vehicle including the Anterior-Posterior Coefficient of Variation (APCV), Medial-Lateral Coefficient of Variation (MLCV), and Deviation of Vehicle Speed but also motion data such as the Jerk-Cost function (JC). A total of 55 drivers including 28 males (age: 24.1 ± 1.5, driving experience: 1.8 years ± 1.7 years) and 27 females (age: 23.8 ± 2.6, driving experience: 1.5 ± 1.0) participated in this study. All subjects were instructed to drive at a constant speed (90 km/h) for 2 min while keeping a distance of 30 m from the front car also running at a speed of 90 km/h. They were requested to drive for the first 1 min and then drive only (Driving Only) or conduct tasks while driving for the subsequent 1 min (Driving + STM or Driving + SN). The information on APCV, MLCV, and deviation of speed were delivered by a driving simulator. Furthermore, the motion signal was measured using 4 high-speed infrared cameras and based on the measurement results, JCs in a total of 6 parts including left shoulder (L.shoulder), left elbow (L.elbow), left hand (L.hand), right knee (R.knee), right ankle (R.ankle), and right toe (R.toe) were calculated. Differences among the results of 3 conditions of experiment, Driving Only, Driving + STM, and Driving + SN, were compared and analyzed in terms of APCV, MLCV, Deviation of Vehicle Speed, and JC. APCV and Deviation of Vehicle Speed increased in Driving + SN, rather than in Driving Only. MLCV increased in Driving + STM and Driving + SN, rather than in Driving Only. In the case of most JCs except that of L.hand, the values increased in Driving + SN, compared to Driving Only. This study indicated that JC could be a reliable parameter for the evaluation of driving performance patterns. In addition, it was discovered that additional tasks under driving, such as STM and SN, impaired smoothness or proficiency in driving motion, thereby increasing anterior-posterior and medio-lateral variability and deviation of speed.

Impact of heterogeneous overlapping drug-eluting stents on the arterial responses of rabbit iliac arteries: a comparison with overlapping bare metal stents.

BACKGROUND AND OBJECTIVES: Although the use of heterogeneous overlapping drug-eluting stents (DES) is not uncommon in clinical practice, whether the implantation sequences of heterogeneous DES will influence the endothelialization or arterial responses differently remains unclear. MATERIALS AND METHODS: Twenty-one rabbits were randomized to receive overlapping stents in the iliac artery for 3 months {distal sirolimus-eluting stent (SES, Cypher™)+proximal paclitaxel-eluting stent (PES, Taxus™) (C+T, n=7), distal Taxus+proximal Cypher (T+C, n=7) and bare metal stent (BMS)+BMS (B+B, n=7)}. Endothelial function was evaluated by the acetylcholine provocation test during follow-up angiography. Histopathological changes in proximal, overlapped, and distal stented segments were evaluated. RESULTS: Although the overall angiographic outcomes were comparable, late loss (mm) in the distal stented segment was higher in the B+B (0.39±0.07) and C+T (0.40±0.20) than that in the T+C (0.06±0.02) group (p<0.001). The incidence of acetylcholine-induced spasm was higher in the DES groups compared with BMS, regardless of the implantation sequences (85.7% in C+T vs. 14.3% in B+B vs. 71.4% in T+C, p=0.017). Notably, only the distal Cypher implantation group (C+T) had three cases of stent fracture. A histopathological analysis showed that despite similar arterial injury scores, Taxus and Cypher stents had higher inflammatory reactions at the overlapped and distal segments compared with those of BMS. CONCLUSION: Despite similar arterial injury, higher inflammatory reactions were observed in overlapping DES segments regardless of the implantation sequence compared with that of BMS. Moreover, DES was associated with impaired endothelial function on the adjacent non-stented segments.