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Objective:To investigate the relationship between preoperative plasma fibrin degradation products (FDP) level and clinicopathological features of patients with completely resected non-small cell lung cancer (NSCLC).Methods:A retrospective case series study was performed. The clinical data of 521 patients who were pathologically diagnosed with NSCLC in Beijing Friendship Hospital Affiliated to Capital Medical University from January 2016 to December 2017 were retrospectively analyzed. Among 521 cases, 406 cases were postoperatively pathologically confirmed as non-lymph node and non-distant metastasis (non-metastasis group) and 115 cases were postoperatively pathologically confirmed as lymph node or distant metastasis (metastasis group). The preoperative FDP level and clinicopathological characteristics as well as the clinicopathological characteristics of NSCLC patients with different FDP levels were compared between the two groups. The correlation between preoperative FDP level and TNM staging was analyzed by using Spearman correlation analysis.Results:Among 521 NSCLC patients, 266 cases were female, 255 cases were male; the age [ M( Q1, Q3)] was 59 years (54 years, 65 years); 441 cases were adenocarcinoma and 70 cases were squamous cell carcinoma. The preoperative median FDP level was 2.78 mg/L (2.35 mg/L, 3.13 mg/L) and 2.99 mg/L (2.56 mg/L, 4.16 mg/L), respectively of NSCLC patients in non-metastasis group and metastasis group, and the difference was statistically significant ( Z = 6.13, P < 0.001). The preoperative FDP level was 2.56 mg/L (2.35 mg/L, 3.20 mg/L) and 2.99 mg/L (2.56 mg/L, 3.20 mg/L), respectively in the early-stage NSCLC (stage Ⅰ-Ⅱ) and advanced NSCLC (stage Ⅲ-Ⅳ) patients, and the difference was statistically significant ( Z = 8.42, P < 0.001). Spearman correlation analysis showed that preoperative FDP level was positively correlated with tumor diameter ( r = 0.287, P < 0.001). There was a positive correlation between preoperative FDP level and the number of metastatic lymph nodes in 115 patients with lymph node metastasis ( r = 0.679, P < 0.001). According to the preoperative median FDP (2.78 mg/L), all patients were divided into FDP ≤2.78 mg/L group and FDP >2.78 mg/L, and there were statistically significant differences in age, metastasis, tumor staging, tumor diameter, the metastatic number of lymph node and histological types of NSCLC patients in both groups (all P < 0.05). Conclusions:The increase of preoperative plasma FDP level may be related to the tumor metastasis and clinical stage of NSCLC patients
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Objective:To investigate the effects of lncRNA associated with microvascular invasion in hepatocellular carcinoma (lncRNA MVIH) on proliferation, invasion and tumorigenic ability of colorectal cancer SW620 cells.Methods:SW620 cells cultured in vitro were divided into empty vector group (transfected with pcDNA3.1 empty vector plasmid), MVIH overexpression group (transfected with pcDNA3.1-MVIH overexpression vector plasmid), NC siRNA group (transfected with interference sequence negative control NC siRNA) and MVIH siRNA group (transfected with MVIH siRNA interference sequence). The mRNA expression level of MVIH in SW620 cells in each group was detected by real-time fluorescence quantitative polymerase chain reaction (qRT-PCR). Cell counting kit-8 (CCK-8), clone formation test and transwell chamber were used to detect the proliferation activity, clone formation ability and invasion ability of SW620 cells; The subcutaneous transplanted tumor of nude mice was constructed by subcutaneous injection of SW620 cells in each group 48 hours after transfection. The transplanted tumor volume was measured every 5 days to evaluate the tumor growth. On the 25th day, the nude mice in each group were killed and the tumor weight was measured. Results:The mRNA MVIH expression level, cell proliferation, clone formation rate and number of invasive cells of SW620 cells in the MVIH overexpression group were significantly higher than those in the empty vector group ( P<0.05); the MVIH mRNA expression level, cell proliferation , clone formation rate and number of invasive cells of SW620 cells in the MVIH-siRNA group were significantly lower than those in NC-siRNA group ( P<0.05). The results of subcutaneous transplantation tumor in nude mice showed that the growth rate of tumor in MVIH overexpression group was faster and the tumor weight was higher than those in the empty vector group ( P<0.05); the growth rate of tumor in MVIH overexpression group was slower and the tumor weight was lower than those in NC-siRNA group ( P<0.05). Conclusions:LncRNA MVIH can promote the proliferation, invasion and tumorigenesis of SW620 cells in colorectal cancer.