TIS111D can affect bladder cancer cells by regulating epithelial-mesenchymal transition.

AIMS: Delineates the role of TIS111D in bladder cancer. MATERIALS AND METHODS: The expression of TIS111D in bladder cancer and adjacent tissues was assessed by immunohistochemistry, Western blot and real-time PCR. Western blot and real-time PCR were used to analyse the expression of TIS111D in HT1197, T24, 5637 and TCCSUP cells. After TIS111D was silenced in T24, 5637 and TCCSUP cells, MTT and Transwell assays were used to detect the effects of TIS111D on proliferation and migration. Western blot and real-time PCR were used to detect the regulatory effect of downregulation of TIS111D on N-cad and E-cad. In vivo experiments confirmed the role of TIS111D in the growth and migration of bladder cancer and determined whether the role of TIS111D in bladder cancer is related to its regulation of N-cad and E-cad. KEY FINDINGS: The expression of TIS11D was higher in tumour tissues and bladder cancer cells. Si-TIS111D could inhibit the growth and migration of bladder cancer cells, while TIS111D could regulate the expression of E-cad and N-cad to regulate epithelial-mesenchymal transition (EMT). We also demonstrated that TIS111D could promote the growth and migration of bladder cancer in vivo by regulating EMT. SIGNIFICANCE: TIS111D may participate in the regulation of bladder cancer progression by regulating EMT.

Chromophobe renal cell carcinoma: clinical and pathological characteristics and prognostic factors in 107 cases / 中华泌尿外科杂志

Objective To analyze the clinicopathological features and prognostic factors of chromophobe renal cell carcinoma.Methods The clinical data of 107 patients with pathologically diagnosed chromophobe cell carcinoma in our hospital from January 2010 to December 2017 were retrospectively analyzed.There were 50 males and 57 females.The age was 16-81 years,with a median age of 53 years.The diameter of the tumor was 1.6-30.0 cm and the median value was 5.0cm.The tumor was located on the right side in 55 cases,on the left side in 51 cases,and bilateral in 1 case.The clinical parameters of different types of renal chromophobe cancer were compared by the Chi square test.The survival analysis was carried out by Kaplan-Meier,and the risk factors were evaluated by single factor and multiple factor Cox proportional risk regression model.Results One hundred and six patients underwent operation,with 55 by open surgery,and 51 by endoscopic surgery,including 89 radical surgery and 17 nephron-sparing surgery.One patient was treated with Sorafenib.Pathological stage T1 stage was 74 cases (69.2％),T2 stage 28 cases (26.2％),T3 stage 3 cases (2.8％),T4 stage 2 cases (1.9％).There were 102 cases of type Ⅰ chromophobe cell carcinoma (95.3％),and 5 cases of type Ⅱ chromophobe cell carcinoma (4.7％).There was no significant difference in gender,age,tumor side,first symptom and T staging in patients with type Ⅰ and Ⅱ chromophobe cell carcinoma (P ＞ 0.05).One hundred and seven patients were followed up for 4-95 months,with the median time of 40 months.Four cases were missed and 5 cases died.The 5 year survival rate was 93.1％.Cox univariate and multivariate regression analysis showed that different T staging and metastasis were independent prognostic factors (all P ＜ 0.05).Conclusions Renal chromophobe cell carcinoma is rare and its diagnosis mainly depends on pathology.Surgery is the main method for the treatment.The malignant grade is low,progress is slow,and the prognosis is good.

MMP1, 2, 3, 7, and 9 gene polymorphisms and urinary cancer risk: a meta-analysis.

BACKGROUND: The matrix metalloproteinases (MMPs) are a family of highly conserved, metal-dependent proteolytic enzymes that play an important role in tumor invasion and metastasis. Many studies have been carried out on the association between polymorphisms in the MMP1, MMP2, MMP3, MMP7, and MMP9 genes and urinary cancer risk. However, the data from these published studies are conflicting and have low statistical power. METHODS: In this study, we performed a meta-analysis of 12 different publications from the PubMed and WanFang databases, published up to May 2015, to better assess the purported associations. Odds ratios (OR) and 95% confidence intervals (CI) were determined to reveal association strengths. RESULTS: Some significant associations were found. For the MMP1 -1607 1G/2G polymorphism, a negative association was identified for the 2G allele in bladder cancer (2G2G+2G1G vs. 1G1G: OR = 0.57, 95% CI = 0.36-0.93, pheterogeneity = 0.001) and renal cell carcinoma (2G1G vs. 1G1G: OR = 0.57, 95% CI = 0.39-0.82, pheterogeneity = 0.567). For the MMP2 -1306 C/T polymorphism, there was a negative association with the T allele for bladder cancer in the Asian population (TT+TC vs. CC: OR = 0.41, 95% CI = 0.18-0.94, pheterogeneity = 0.195). For the MMP7 -181 A/G polymorphism, a decreased bladder cancer risk was found (G-allele vs. A-allele: OR = 0.81, 95% CI = 0.66-0.98, pheterogeneity =0.325). CONCLUSION: In summary, our study showed evidence that genetic polymorphisms in MMP1 for all populations, but only in the Asian population for MMP2 and MMP7, may protect against bladder cancer risk. Future studies with larger sample sizes are warranted to further evaluate these associations in more detail.

Effects of silencing of iASPP gene on human bladder cancer cells / 中华泌尿外科杂志

Objective To discuss the effects of silencing of iASPP gene on human bladder cancer cells. Methods RNAi silencing of iASPP gene in bladder cancer cell 5637 and T24 cells were used by lentiviral mediated interfering short hairpin RNAs. Cell proliferation was tested by MTT assay, and rate of colony was tested by colony formation assay. Cell cycles were tested by using fluorescence-activated cell sorting. Results Down-regulation of iASPP could inhibit the growth and proliferation of human bladder cancer cells (P＜0.05). iASPP know-down could decrease the colony formation of 5637 and T24 cells (P＜0, 05). Knocking down of iASPP in 5637 and T24 cells showed cell arrested at G1. Conclusions Silencing of iASPP gene could inhibit proliferation and colony formation of bladder cancer, iASPP might be an important target for gene therapy of bladder cancer.

The Clinical Significance of Treating Respiratory Failure Secondary to the Acute Exacerbation of Chronic Obstructive Pulmonary Diseases through Bilevel Positive Airway Pressure Ventilation via Nasal/mouth Mask / 昆明医科大学学报

Objective To explore the effectiveness of bilevel positive airway pressure(Bipap)ventilation via nasal/ mouth mask in patients with respiratory failure secondary to the acute exacerbation of chronic obstructive pulmonary diseases(COPD).Methods 40 patients were randomly submitted either to standard therapy(consisting of medical,oxygen and physical therapy)plus BiPAP or to standard therapy.Clinical manifestation were comparatively analyzed before and after 4 hours and 7 days of treatment in each group.Results After 4 hours and 7 days of treatment by BiPAP,patients showed a significant improvement in PaCO_2,and clinical manifestation,and there was a significant difference between the two groups.Conclusion Bipap ventilation is effective in patients with respiratory failure during acute exacerbation of COPD.