Serum high-density lipoprotein level and prognosis of ovarian cancer.

This study aimed to investigate the prognostic value of serum high-density lipoprotein (HDL) level in patients with ovarian cancer. This study enrolled 152 patients diagnosed with ovarian cancer and 119 patients with benign ovarian tumors. The associations of patient characteristics and disease with survival were determined using Cox regression analysis, t tests, analysis of variance for multiple-group comparisons, and chi-square tests. The potential association between HDL levels and the clinical characteristics of the disease was also analyzed. The diagnostic value of HDL was estimated using receiver operating characteristic curve analysis and calculation of the area under the curve. Progression-free survival and overall survival were determined using the Kaplan-Meier method, and their associations with patient and pathological variables, including HDL, were determined using the log-rank test. The median serum HDL was 1.15 mm measured in 152 patients with ovarian cancer and 1.30 mm in 119 patients with benign ovarian tumors (P = .000054). The receiver operating characteristic curve analysis yielded an area under the curve of 0.735 for serum HDL levels. Serum HDL levels were significantly associated with tumor pathological types (non-serous vs serous, P < .05). No association was observed between serum HDL levels and patient age, age at menarche or marriage, number of children, tumor grade, or clinical stage (P > .05). Patients with high serum HDL levels had a longer progression-free survival and overall survival than those with low serum HDL levels. Serum HDL levels are an independent prognostic factor for ovarian cancer.

Application progress of black phosphorus nanomaterials in biomedicine / 国际生物医学工程杂志

Black phosphorus, as a novel two-dimensional nanomaterial, has received a lot of attention from researchers for its unique structure and properties. In recent years, with the increasing cross-sectional research related to black phosphorus 2D nanomaterials in various fields such as materials science, physics, chemistry, biology, and medicine, it has shown great potential for development and application in biomedicine. The excellent photoacoustic properties and good biocompatibility of black phosphorus 2D nanomaterials make them outstanding in tumor diagnosis and treatment. In this paper, the structure and properties, preparation, and functional modification of black phosphorus two-dimensional nanomaterials and their potential applications in the bio-detection and treatment of tumors, as well as the application progress of antibacterial were reviewed.

Efficacy and influencing factors of the four-step approach combining the situational simulation teaching method in the clinical practice of standardized training for residents.

Background and Aims: Clinical skills practice is an essential component in standardized residency training. However, traditionally skill training methods are dogmatic and not all residents are exposed to such prescribed situations during their residency. The aims of this study were to evaluate the effectiveness and influence factors of a four-step approach combining situational simulation teaching methods in clinical practice for residents. Methods: Enrolled all second-year residents from the internal medicine base between May 2017 and May 2018 (n = 94), randomly divided into two groups. Forty-eight residents were selected as experimental group, while the others 46 as the control group. Adopted traditional clinical practice method in the teaching and assessment of the control group, while used four-step approach combining situational simulation teaching method in experimental group. We compared the theoretical and skill assessment scores in preclass and postclass. Conducted a satisfaction survey after class and analyzed the influencing factors of the teaching effect evaluation. Results: There were no significant differences in the theoretical and skill assessment scores between experimental group and control group at the beginning. After the class, both the average skill assessment and Direct Observation of Procedural Skills scores of the experimental group were higher than those of the control. Satisfaction survey findings identified that the experimental group expressed higher satisfaction. Logistic regression showed that educational background, "situational simulation mode helps to improve clinical skills training," "helps to maintain attention during learning," and "helps improve the ability to exercise analysis and solve problems" were the influencing factors of learners' satisfaction. Conclusion: The application of four-step approach combining situational simulation teaching methods in the clinical practice of residents can significantly improve skills, thinking ability, decision-making ability, and teaching satisfaction. Therefore, four-step approach combining situational simulation teaching methods is worth promoting in teaching clinical skills for internal medicine residency training.

Excited-State Dynamics of [Ru(S-Sbpy)(bpy)2]2+ to Form Long-Lived Localized Triplet States.

The novel photosensitizer [Ru(S-Sbpy)(bpy)2]2+ harbors two distinct sets of excited states in the UV/Vis region of the absorption spectrum located on either bpy or S-Sbpy ligands. Here, we address the question of whether following excitation into these two types of states could lead to the formation of different long-lived excited states from where energy transfer to a reactive species could occur. Femtosecond transient absorption spectroscopy identifies the formation of the final state within 80 fs for both excitation wavelengths. The recorded spectra hint at very similar dynamics following excitation toward either the parent or sulfur-decorated bpy ligands, indicating ultrafast interconversion into a unique excited-state species regardless of the initial state. Non-adiabatic surface hopping dynamics simulations show that ultrafast spin-orbit-mediated mixing of the states within less than 50 fs strongly increases the localization of the excited electron at the S-Sbpy ligand. Extensive structural relaxation within this sulfurated ligand is possible, via S-S bond cleavage that results in triplet state energies that are lower than those in the analogue [Ru(bpy)3]2+. This structural relaxation upon localization of the charge on S-Sbpy is found to be the reason for the formation of a single long-lived species independent of the excitation wavelength.

An analysis of insomnia and its influencing factors in patients with acute coronary syndrome / 中华内科杂志

Objective:To investigate the current situation of insomnia in patients with acute coronary syndrome (ACS), and analyze the influencing factors of insomnia in the ACS patients, so as to provide information on the development of new strategies for the treatment of insomnia in ACS patients.Methods:This is a multicenter and prospective observational study. A total of 771 ACS patients who met the criteria were selected from March 2013 to June 2015. The baseline social demographic information, sleep quality questionnaire, general anxiety disorder scale-7(GAD-7),patient health questionnaire-9(PHQ-9), short-form 12 health survey questionnaire(SF-12), and enhancing recovery in coronary heart disease patients social inventory(ESSI) were completed within 7 days after admission. Logistic regression analyses were used to analyze the influencing factors of insomnia in ACS patients.Results:A total of 741 subjects with valid questionnaires were collected, including 510 males (68.8%) and 231 females (31.2%). Among them, 487 (65.7%) subjects had at least one insomnia symptom: 308 (41.6%) subjects had difficulty in falling asleep, 369 (49.8%) subjects were easy to wake at night, 116 (15.7%) subjects woke up earlier than they expected, 74 (10.0%) subjects experienced both woke up earlier and difficulty in falling asleep, and 53 (7.2%) subjects woke up earlier, woke up at night and had difficulty in falling asleep at the same time. Logistic regression analyses showed that before admission physical activity ( OR =0.636, 95% CI 0.411-0.984), depression ( OR=1.908, 95% CI 1.101-3.305) and low social support ( OR=0.278, 95% CI 1.198-3.301) were independent factors of insomnia in ACS patients. Conclusions:Nearly 2/3 ACS patients have symptoms of insomnia. Difficulty in falling asleep and easy to wake up at night are the most common manifestations. Physical activity, depression and social support independently are associated with insomnia.

Time-series plasma cell-free DNA analysis reveals disease severity of COVID-19 patients

Clinical symptoms of coronavirus disease 2019 (COVID-19) range from asymptomatic to severe pneumonia and death. Detection of individuals at high risk for critical condition is crucial for control of the disease. Herein, for the first time, we profiled and analyzed plasma cell-free DNA (cfDNA) of mild and severe COVID-19 patients. We found that in comparison between mild and severe COVID-19 patients, Interleukin-37 signaling was one of the most relevant pathways; top significantly altered genes included POTEH, FAM27C, SPATA48, which were mostly expressed in prostate and testis; adrenal glands, small intestines and liver were tissues presenting most differentially expressed genes. Our data thus revealed potential tissue involvement, provided insights into mechanism on COVID-19 progression, and highlighted utility of cfDNA as a noninvasive biomarker for disease severity inspections. One Sentence SummaryCfDNA analysis in COVID-19 patients reveals severity-related tissue damage.

Development of Stand-walking Simulator for Rehabilitation Training / 中国医疗器械杂志

A rehabilitation equipment is developed by mechanical and electronic engineering principle based on the need of rehabilitation training for patients with paraplegia. The device can prevent complications such as palsy bedsores and deep venous thrombosis. The device effectively reduces the labor intensity of the rehabilitation staff or family members to carry the patient's body, and promotes the physical and psychological rehabilitation progress of patients with lower limb paralysis.

Research on Chemical Equivalence Characteristics of Polylactic Acid in Medical Devices / 中国医疗器械杂志

Polylactic acid is synthesized indirectly by the polymerization method, according to the standard GB/T 16886.18-2011, the evaluation parameters and methods about chemical characterization of polylactic acid have been established. By using rigorous and comprehensive comparative analysis, the chemical equivalency of domestic and imported polylactic acid materials has been proved, along with the "Medical Device Biology Evaluation and Review Guide", paving the way of using domestic polylactic acid in implantable medical devices.

An Analysis of Auditory Perception and Speech Ability Outcomes after Cochlear Implantation in Prelingually Deaf Children with Leukoencephalopathy / 听力学及言语疾病杂志

Objective To study the auditory perception and speech ability outcomes after cochlear implantation in prelingually deaf children with extremely severe neurosensory hearing loss and comorbid leukoencephalopathy.Methods Our study included 14 prelingually deaf children with leukoencephalopathy (confirmed by preoperative MRI scanning) who were treated with cochlear implantation in the Department of Otorhinolaryngology Head and Neck Surgery of Hainan Provincial People''s Hospital, including 8 males and 6 females of 1~6 yr with a mean age of 3.8 yr.Sixteen synchronous prelingually deaf children without central nervous system (CNS) diseases were also included as the control group, including 11 males and 5 females of 1~6 yr with a mean age of 4.4 yr.All the patients underwent pre-surgical assessments of audiology, radiology, speech ability and intelligence before cochlear implantation via transmastoid facial nerve recess approach was done, after which rehabilitation was provided at the Hainan Rehabilitation Center for Deaf Children.Categories of auditory performance (CAP) and speech intelligibility rate (SIR) were employed as the assessment criteria for the outcome of surgery, the scores of which at different timepoints after surgery were statistically compared through paired t-test between the two groups.Results All the patients gained post-surgical hearing and speech abilities to different degrees, whereas CAP and SIR scores both chronologically improved in the both groups.No statistical difference was revealed either in CAP or in SIR scores between the two groups at 6, 12 and 24 months after surgery (P>0.05).Conclusion Children with extremely severe neurosensory hearing loss can be reated with cochlear implantation even if they have comorbid leukoencephalopathy.The effects of auditory and speech rehabilitation for the same age patients with leukoencephalopathy are similar to those without leukoencephalopathy in 2 years after surgery.

Analysis of dermoscopy and reflectance confocal microscopy features of verruca plana / 中华皮肤科杂志

Objective To evaluate imaging features of verruca plana by dermoscopy and reflectance confocal microscopy (RCM).Methods Totally,88 untreated patients with verruca plana were enrolled into this study.One typical skin lesion was chosen from each patient and subjected to dermoscopy and RCM separately,and imaging features were recorded and analyzed.Results Dermoscopy revealed a light red background in 48 cases (54.55％),a light yellow background in 40 cases (45.45％),and a various number of punctate hemorrhages in 84 cases (95.45％).RCM showed concentric onion-skin-like structures in 48 patients (54.55％).Among 57 patients with disease duration ＞ 1 year,44 (77.19％) had the concentric onion-skin-like pattern,while only 4 (12.90％) patients had similar structures among 31 patients with disease duration ＜ 1 year.A significant difference in the prevalence of concentric onion-skin-like structures was observed between patients with disease duration ＞ 1 year and those with disease duration ＜ 1 year (x2 =33.47,P ＜ 0.05).Of 88 patients,86 (97.73％) had hyperpigmentation in the basal layer at the dermo-epidermal junction,intact dermal papillary rings,vasodilatation,hyperemia and 1-2 dilated capillaries in the dermal papillae.Eight patients were treated for 2 weeks,and the above features disappeared after the treatment under dermoscopy and RCM.Conclusion Based on clinical manifestations,dermoscopy and RCM features may be helpful for the diagnosis of verruca plana.

Effects of low temperature compound propofol on expression and ultrastructural changes of hippocampal neurons apoptosis proteinin rats / 重庆医学

Objective To observe the effect of low temperature compound propofol on the changes of apoptosis protein Caspase-3,autophagy-related proteins Beclin-1 and LC3-Ⅱ and thier changes of hippocampal neurons.Methods The rats were randomly divided into blank control group (Group A),propofol group at low temperature (Group B) and chloral hydrate group at low temperature (Group C),Group B and C were treated with low temperature for 30 min.Then,each group was subjected to cardiac perfusion and decapitated brain to prepare rat hippocampal neuronal tissue samples.The expression of Caspase-3 was detected by immunohistochemistry,Beclin-1 and LC3-Ⅱ protein was detected by immunoblotting.The ultrastructural changes of neurons were observed by transmission electron microscopy.Results The expression of Caspase-3,Beclin-1 and LC3-Ⅱ protein in each group were statistically significant differences(P＜0.05).The results of transmission electron microscopy showed that the neurons in group B and C were changed in different degrees,especially in group C neuronal apoptosis is obvious.Conclusion Autophagy and apoptosis in existence still exist in low temperature condition,while propofol can reduce this damage and have better protective effect on neurons.

The study of diffusion tensor imaging and cognitive function in patients with asymptomatic mild traumat-ic brain injury / 中国神经精神疾病杂志

Objective To explore the features of white matter in DTI in patients with asymptomatic mild traumat?ic brain injury(mTBI)and their correlation with the cognitive features. Methods The DTI data and cognitive function data were obtained from 36 mTBI patients and 36 health controls. The fractional anisotropy (FA) pictures were ana?lyzed by voxel-based analysis. The FA value differences of the two groups were analyzed by using t test. The values of FA were extracted from the abnormal regions of mTBI patients. Correlation analyses were performed on the association of extracted FA value with P300 latency,P300 amplitude,the scores of Montreal Cognitive Assessment (MoCA) Scale, Hamilton Depression Scale, Hamilton Anxiey Scale,age and education(P>0.05). Results Compared with controls, mTBI patients had significant reduction of FA in the right medial frontal gyrus, bilateral postcentral gyrus, left precune?us, right posterior cingutate and right superior temporal gyrus(P<0.01,FDR correction). There were no significant correlations between extracted FA value and all clinical characteristics in mTBI patients. Conclusions Patients with mTBI have white matter microstructural damage and cognitive impairment.

A structural equation modeling of caregiving experiences of family caregivers caring for people with dementia / 中国心理卫生杂志

Objective:To explore and test a caregiving experience model by Structural Equation Modeling (SEM)in the family caregivers caring for people with dementia in China. Method:Totally 349 elder patients with dementia and their family caregivers were selected and interviewed,using a battery of measures including demo-graphic data of the dyads,impairement of care recipient,caregiver's appraisal of role strain,role gain,negative and positive well-being outcomes. By using the Kramer's caregiver adaptation model as theoretical framework,a care-giving experience model were hypothesized tested by structural equation modeling (SEM). Result:A five-factor model for the caregiving experience was supported by SEM. The model demonstrated that the severity of dementia significantly influenced the caregiver's appraisal of role strain (β=0. 5 1 ,P<0. 05 ),which predicted indirectly neg-ative well-being outcomes of the caregiver (β=0. 63,P<0. 05). On the other hand the caregiver's appraisal of rolegain which was negatively influenced by the severity of parent's dementia (β=-0. 33,P<0. 05),predicted posi-tive well-being outcomes of the caregiver (β=0. 75 ,P<0. 05 ). Conclusion:It suggests that both positive and nega-tive aspects of caregiving experiences could independently impact on the well-being outcomes of adult-child care-givers,and therefore both aspects should be targeted in supportive interventions for family caregivers in China.

The combination ofKangfuxing liquid, ethacridine lactate and local oxygen therapy in the treatment of radiation-induced skin damage / 国际中医中药杂志

Objective To investigate the efficiency ofKangfuxing liquid, ethacridine lactate in combination with local oxygen therapy for radiation-induced skin damage.Methods A total of 96 patients affected by radiation-induced skin damage were enrolled and randomly allocated to a treatment group and a control group by random number table method, with 48 patients in each group. All patients in the control group were treated with trolamine cream, and those in the treatment group were treated withKangfuxing liquid, ethacridine lactate in combination with local oxygen therapy. Skin dermatitis was scored utilizing Radiation Therapy Oncology Group (RTOG) toxicity scale.Results The total effective rate in the treatment group was significantly higher than that in the control group (97.9%vs.79.1%;χ2=10.119,P=0.002). The distribution of patients with RTOG grades 0 to 4 showed different degrees of improvement, and the number of patients with RTOG 0-4 grades changed from 0, 3, 9, 12, 24 before the treatment to 8, 12, 22, 6, 0 after the treatment in the treatment group, 0, 2, 10, 25, 11 to 2, 5, 18, 23, 0 in the control group. The percent of patients with RTOG grade 0 in the treatment group was significantly increased than that in the control group (16.7%vs. 4.2%;χ2=4.019, P=0.045) and the percent of patients with RTOG grade 3 significantly decreased (12.5%vs.47.9%;χ2=12.649, P<0.001). The percents of patients with dispirited spirit (31.3%vs. 58.3%;χ2=6.066,P=0.014), dry and chapped skin (20.8%vs. 58.3%;χ2=12.588,P=0.000) in the treatment group were all significantly lower than those in the control group.ConclusionKangfuxing Liquid, ethacridine lactate in combination with local oxygen therapy can improve radiation-induced skin damage.

Investigation and Analysis on the Content of Pharmacology Course for Pharmacy Major in Higher Vocational Colleges / 中国药房

OBJECTIVE:To establish the system of curriculum based on working process and occupation ability for pharmacy major in higher vocational colleges. METHODS:Investigation was conducted among medical practitioners from pharmaceutical companies,hospitals,pharmaceutical factories,scientific research institutions and other related professionals. RESULTS:150 ques-tionnaires were sent out,and 141 valid questionnaires were collected with effective recovery rate of 94.00%. Results of investiga-tion showed that respondents most valued graduates with interpersonal and communication skills,followed by professional skills and practical ability. They were mainly clinical application of drugs,pharmacological effects and adverse reactions of drugs in the pharmacology theory teaching,the mechanism of action of drugs were weakened. The ability of prescription distribution,symptoms inquiring and drugs recommending should be strengthened in the pharmacology theory practice teaching. More than half of the re-spondents thought that confirmatory tests were necessary to keep,which helped to train students’practical ability and deepen the understanding of the theory. Meanwhile,it was important to strengthen the students’communication with the patients or their fami-lies and doctors to cultivate the ability of acquiring professional knowledge. CONCLUSIONS:The investigation provides basis for the making of curriculum standards of pharmacology,through which teaching contents are selected,teaching methods are de-signed,and it makes the pharmacology course full of post applicability and provides better decision-making basis to meet the posi-tion requirements.

Risk factor analysis of recurrence of benign paroxysmal positional vertigo after manipulative reduction / 中国综合临床

Objective To investigate the recurrence rate and recurrence risk factors of benign positional vertigo after manual reduction. Methods A total of 186 cases diagnosed with BPPV were selected as our subjects. Vertigo recurrence rate was calculated at 30 d,60 d,90 d after manual reduction. Risk factors for recurrence were analyzed using binary Logistic Regression. Results 172 cases diagnosed in BPPV patients completed 90 days follow-up. Vertigo recurrence rate was 8. 79%(16/182)in 30 days,17. 05%(30/176)in 60 days,and 22. 09%(38/172)in 90 days. The average age in recurrence patients was(59. 1 ± 10. 2)years old,significantly higher than the non-recurrent group( 47. 6 ± 7. 3 ). The recurrence rates in patients with complicated with hypertension,diabetes,hyperlipidemia,coronary heart disease were 57. 89%(22/38),60. 53%(23/38),73. 68%(28/38),39. 47%(15/38)respectively. Rise and turning neck are prone to recurrence,and recurrence rate were 16. 42%( 22/134 ),8. 21%( 11/134 ),9. 70%( 13/134 ) and 16. 42%(22/134). Recurrence rate of patients with manipulative reduction times ﹥10 times( reset multiple times before being cured)was 90. 90%( 10/11 ). Logistic regression analysis showed that the duration before manual reduction BPPV(OR=2. 631,95%CI 1. 082 -7. 572,P =0. 022)and the number of manual reduction ﹥10( OR=3. 107,95%CI 1. 205-7. 842,P=0. 018)after BPPV is manipulative reduction independent risk factor for recurrence. Conclusion Patients with BPPV in middle-aged and high seizure frequency have a high recurrence rate. Patients with BPPV complicated by hypertension,diabetes,hyperlipidemia,coronary atherosclerotic heart disease have the same higher recurrence rate. The rise and turn the neck often cause BPPV recurrence. The reduction and the number of manual reduction﹥10 are independent risk factors of recurrence of the BPPV.

Determination of artesunate in artesunate and amodiaquine hydrochloride tablets by HPLC / 中国生化药物杂志

Objective To establish an HPLC method for the determination of artesunate in artesunate and amodiaquine hydrochloride tablets. Methods WondaSil C18-WR column was used with mobile phase consisted of acetonitrile:phosphoric acid aqueous solution(adjust pH to 3,gradient elution);wavelength was 210 nm; flow rate was 1 mL/min and the column temperature was 30℃.Results The standard curve was linear in the range of 0.2~3.2 mg/mL(r=0.9997), average recoveries were 99.0%(RSD=1.35%, n=6).Conclusion The method is accurate and sensitive, and it can be used to control the quality of artesunate and amodiaquine hydrochloride tablets.

Effect of Electroacupuncture plus Early Rehabilitation Training on Function Recovery after Surgery for Cervical Spinal Cord Injury / 上海针灸杂志

Objective To investigate the effect of electroacupuncture plus early rehabilitation training on the function recovery after surgery for cervical spinal cord injury. Methods Sixty patients with cervical spinal cord injury undergone anterior cervical decompression and internal fixation were randomized into a treatment group and a control group, 30 cases in each group. The treatment group was intervened by electroacupuncture plus rehabilitation training, while the control group was by rehabilitation training alone. The Frankel’s grading (FG), Barthel Index (BI), and Functional Independence Measure (FIM) were adopted for evaluation before and after intervention. Results The FG was not significantly changed after intervention in both groups (P>0.05). After intervention, there was no significant difference in comparing FG between the two groups (P>0.05). The BI and FIM scores were significantly changed after intervention in both groups (P＜0.01). There were significant differences in comparing BI and FIM scores between the two groups (P＜0.05). Conclusion Electroacupuncture plus early rehabilitation training can promote the function recovery after surgery for cervical spinal cord injury, and improve the quality of life.

Clinical research of sudden sensorineural hearing loss due to inner-ear hemorrhage / 中华耳鼻咽喉头颈外科杂志

<p><b>OBJECTIVE</b>This study addresses the characters of magnetic resonance imaging (MRI) and clinical features, and curative effects in the screening of SSNHL due to inner-ear hemorrhage.</p><p><b>METHODS</b>MRI and relevant audiometric test were given to 160 patients with SSNHL, who were hospitalized in the First Affiliated Hospital of Sun Yat-sen University from January 2011 to April 2013. The clinical features and curative effects of patients with high signals in the labyrinth on MRI were analyzed.</p><p><b>RESULTS</b>MRI abnormities were found in 22 (13.8%) of the patients. Specifically, eight cases were considered for inner-ear hemorrhage. For the eight inner-ear hemorrhage cases, clinical features included the sudden onset of complete hearing loss, which worsened within several hours. Pure tone audiometry indicated profound sensorineural deafness. The prevalence of inner-ear hemorrhage was 5% in SSNHL cases and 18.6% in cases of profound sensorineural. MRI showed high signal intensity in the cochlear, semicircular canals or vestibule on unenhanced T1-weighted and fluid-attenuated inversion recovery images. The high signal intensity in the inner ear gradually degraded in six months. There was no improvement in hearing for the patients with inner-ear hemorrhage following symptomatic therapy.</p><p><b>CONCLUSION</b>SSNHL due to inner-ear hemorrhage is characterized by profound sensorineural deafness in all frequencies, and high signal intensity for the hemorrhagic inner ear on T1-weighted MRI, with poor prognosis.</p>

Research on the progress of Berberine determination / 中国生化药物杂志

Berberine is the main active ingredients in Coptis.The content determination of berberine can provide the basis for its quality evaluation.Relevant provessinal literatures about determination methods of berberine were summarized,and the main methods included TLC,UV, HPLC,HPEC,fluorescence,and so on.HPLC was the most widely used method because of its high separation efficiency,specificity and sensitivity.