RESUMO
Osteoporosis is a major concern in patients with Duchenne muscular dystrophy. In this novel study of teriparatide treatment in 6 patients with severe osteoporosis, bone health (fractures, vertebral morphometry, and DXA) remained stable, with no adverse events. These findings will help inform future osteoporosis research in this challenging population. INTRODUCTION: Despite standard therapy with vitamin D and bisphosphonates (BP), many patients with Duchenne muscular dystrophy (DMD) continue to sustain fragility fractures due to long-term glucocorticoid treatment and limited mobility. We aimed to evaluate the safety and efficacy of teriparatide for the treatment of severe osteoporosis in adolescent and young adult patients with DMD. METHODS: We prospectively treated 6 patients with DMD who had severe osteoporosis with teriparatide 20 mcg subcutaneously daily for 1-2 years. Inclusion criteria were long-term glucocorticoid therapy, and severe osteoporosis despite treatment with BP, or intolerance to BP. We examined long bone and vertebral fracture outcomes, including vertebral morphometry measures, bone mineral density and content, bone formation markers, safety indices, and adverse events. RESULTS: The mean age at teriparatide start was 17.9 years (range 13.9-22.1 years). All 6 patients were on daily glucocorticoids (mean ± SD; duration 10.9 ± 2.5 years) and 5 were non-ambulatory. Five patients had been treated with BP for 7.9 ± 4.2 years. All had vertebral and a history of long bone fragility fractures at baseline. Vertebral heights and Genant fracture grading remained stable. Long bone fracture rate appeared to decrease (from 0.84/year to 0.09/year); one patient sustained a long bone fracture at 6 months of treatment. Trajectories for change in bone mineral density and content were not different post- vs. pre-teriparatide. Procollagen type 1 amino-terminal propeptide (P1NP) increased, while laboratory safety indices remained stable and non-concerning. No adverse events were observed. CONCLUSION: In six patients with DMD treated with teriparatide for severe osteoporosis, we observed stable bone health and modest increases in P1NP, without safety concerns. Further studies are needed to better understand teriparatide efficacy for treatment of osteoporosis in patients with DMD.
Assuntos
Conservadores da Densidade Óssea , Distrofia Muscular de Duchenne , Osteoporose , Adolescente , Adulto , Densidade Óssea , Conservadores da Densidade Óssea/efeitos adversos , Humanos , Distrofia Muscular de Duchenne/complicações , Distrofia Muscular de Duchenne/tratamento farmacológico , Osteoporose/tratamento farmacológico , Teriparatida/uso terapêutico , Adulto JovemRESUMO
This is the first report on safety and efficacy of intravenous bisphosphonates (IV BP) for treatment of disuse osteoporosis and low bone mineral density (BMD) in children with spinal muscular atrophy (SMA). IV BP appears to be safe and effective in fracture rate reduction. However, caution is necessary given the occurrence of an atypical femur fracture. INTRODUCTION: Children with SMA are at high risk for fragility fractures and low BMD. IV BP have been used for treatment of disuse osteoporosis in pediatrics. However, safety and efficacy of IV BP in the SMA population has not been reported. METHODS: Retrospective chart review of IV BP for treatment of disuse osteoporosis and low BMD in children with SMA at a tertiary pediatric center from 2010 to 2018 RESULTS: Eight patients (50% female; 75% SMA type 1; median age at first infusion 6.7 years) receiving a total of 39 infusions (54% pamidronate, 46% zoledronic acid) were included in this report. Acute phase reactions occurred following 38% and 3% of initial and subsequent infusions, respectively. BMD trended toward improvement at 1 year post-treatment. Among six patients who had > 2 years of follow-up, fracture rate decreased from 1.4 to 0.1 fracture/year. An atypical femur fracture was observed in one patient. CONCLUSION: These findings suggest that in children with SMA, IV BP therapy appears to be safe with minimal acute side effects and effective to reduce fracture rate. Caution is still needed given the occurrence of an atypical femur fracture in SMA population.
Assuntos
Conservadores da Densidade Óssea , Fraturas Ósseas , Atrofia Muscular Espinal , Osteoporose , Densidade Óssea , Conservadores da Densidade Óssea/efeitos adversos , Criança , Difosfonatos/efeitos adversos , Feminino , Humanos , Masculino , Osteoporose/tratamento farmacológico , Estudos RetrospectivosRESUMO
We present Monte Carlo simulations of the gamma exposure in closed rooms made of steel or concrete and contaminated by 6°Co or NORM radionuclides. The computer code PENELOPE-2008 (Salvat et al., 2009) was used. Our simulations for 6°Co suggest considering detailed Monte Carlo simulations in future recommendations on clearance and exemption of materials with low radioactivity. For NORM nuclides our calculations suggest that Monte Carlo simulations are a possible alternative in case a material fails the dose rate criteria by using the RP 112 screening method.
Assuntos
Radioisótopos de Cobalto/efeitos adversos , Materiais de Construção/efeitos adversos , Raios gama/efeitos adversos , Simulação por Computador , Humanos , Método de Monte Carlo , Radioisótopos/efeitos adversos , Software , Aço/efeitos adversosRESUMO
In emergency situation or in case of defence against nuclear hazards, the rapid analysis of radioisotopes in food products is essential. Radiostrontium is one of the most interesting isotopes in case of emergency. The determination of radiostrontium in milk and dairy products plays an important role especially for infants. The procedures described here were tested for fast determination of (90)Sr. The typical chemical recovery of the proposed fast procedure for determination of strontium from milk and dairy products was 90% and the time needed for analysis was one working day. The achieved detection limit for milk is 0.8Bq/l. An ultra fast screening method allows the determination of radiostrontium with quantitative recovery within 1 hour. The minimum detectable activity in this case is 230Bq/l.
Assuntos
Laticínios/análise , Monitoramento Ambiental/métodos , Poluentes Ambientais/análise , Contaminação Radioativa de Alimentos/análise , Leite/química , Radioisótopos de Estrôncio/análise , Animais , Fatores de TempoRESUMO
Few identification methods will rapidly or specifically detect all bacteria in the order Chlamydiales, family Chlamydiaceae. In this study, three PCR tests based on sequence data from over 48 chlamydial strains were developed for identification of these bacteria. Two tests exclusively recognized the Chlamydiaceae: a multiplex test targeting the ompA gene and the rRNA intergenic spacer and a TaqMan test targeting the 23S ribosomal DNA. The multiplex test was able to detect as few as 200 inclusion-forming units (IFU), while the TaqMan test could detect 2 IFU. The amplicons produced in these tests ranged from 132 to 320 bp in length. The third test, targeting the 23S rRNA gene, produced a 600-bp amplicon from strains belonging to several families in the order Chlamydiales. Direct sequence analysis of this amplicon has facilitated the identification of new chlamydial strains. These three tests permit ready identification of chlamydiae for diagnostic and epidemiologic study. The specificity of these tests indicates that they might also be used to identify chlamydiae without culture or isolation.
Assuntos
Infecções por Chlamydiaceae/diagnóstico , Chlamydiaceae/classificação , Chlamydiaceae/isolamento & purificação , Chlamydiales/classificação , Chlamydiales/isolamento & purificação , Infecções por Bactérias Gram-Negativas/diagnóstico , Reação em Cadeia da Polimerase/métodos , Proteínas da Membrana Bacteriana Externa/genética , Chlamydiaceae/genética , Chlamydiales/genética , Primers do DNA , DNA Ribossômico/genética , Humanos , Íntrons , RNA Bacteriano/genética , RNA Ribossômico 23S/genética , Sensibilidade e EspecificidadeRESUMO
A research programme sponsored by the German Federal Ministry for the Environment, Nature Protection and Nuclear Safety (BMU) was conducted during 1992 and 1993 in the Southern Urals, to provide an initial validation and comparison of results of population exposure arising from the release of radioactive waste from the MAYAK nuclear facility between the years 1948 and 1967. This programme included investigations of the contamination of the soil, of food (milk, drinking-water, potatoes) and whole-body-counter measurements of inhabitants of settlements at the Techa River. The nuclides of interest were plutonium isotopes and the long-lived fission products 137Cs and 90Sr. Results of these investigations, particularly in and around the village of Muslyumovo (78 km downstream from the point of release of the radioactive waste into the Techa River), are shown. These investigations are a first step towards an independent validation of the enormous data base collected by the Russian institutes and of derived values of the doses to the population of the Techa River.