Increasing the capacity of water distribution networks using fitness function transformation.

Even the most carefully designed water distribution network (WDN) can suffer from local capacity deficiencies as a result of the quick and unpredictable growth of the urbanization of new industrial sites. To solve this problem, this paper focuses on the identification of the best possible location for a new pipeline within an existing WDN, which maximizes the node-wise capacity. To determine the optimal solution, a parameter, namely pressure sensitivity, is defined, which can localize nodes with local capacity problems computationally efficiently. During our research, a fitness function transformation technique was defined, which increases the effectivity of the optimization on a larger scale by the formulation of a feasible fitness function. Combining this technique with an extended version of the genetic algorithm, the topology of eleven real-life WDN was optimized. A scrutiny is performed on three networks, highlighting typical deficiencies.

Label-free tracking of whole-cell response on RGD functionalized surfaces to varied flow velocities generated by fluidic rotation.

Fluidic flow plays important roles in colloid and interface sciences. Measuring adsorption, aggregation processes and living cell behavior under a fluidic environment with varied flow velocities in a parallel and high-throughput manner remains to be a challenging task. Here a method is introduced to monitor cell response to well-defined flow with varied velocities over an array of label-free resonant waveguide grating (RWG) based optical biosensors. The arrangement consists of a circular well with an array of biosensors at the bottom surface. By rotating the liquid over the biosensor array using a magnetic stirrer bar, flow velocities from zero to a predefined maximum can be easily established over different locations within the biosensor array as characterized in detail by numerical simulations. Cell adhesion and detachment measurements on an Arg-Gly-Asp (RGD) peptide functionalized surface were performed to demonstrate i) measurements at a wide range of simultaneous flow velocities over the same interface; ii) the possibility of parallel measurements at the same flow conditions in one run; and iii) the simple tuning of the employed range of flow velocities. Our setup made it possible to analyze the magnitude and rate of cell detachment at various flow velocities in parallel and determine the critical velocity and force where cells start to detach from the RGD motif displaying biomimetic surface. Furthermore, cellular response to simultaneous mechanical (flow) and chemical stimulation was also investigated using trypsin as a model. This study opens a new possibility to investigate interface phenomena under predefined and conveniently varied flow conditions.

Vulnerability analysis of water distribution networks to accidental pipe burst.

Even the best-maintained water distribution network (WDN) might suffer pipe bursts occasionally, and the utility company must reconstruct the damaged sections of the system. The affected area must be segregated by closing the corresponding isolation valves; as a result, the required amount of drinking water might not be available. This paper explores the behaviour and topology of segments, especially their criticality from the viewpoint of the whole system. A novel, objective, dimensionless, segment-based quantity is proposed to evaluate the vulnerability of both the segments and the whole WDN against a single, incidental pipe break, computed as the product of the probability of failure within the segment and the amount of unserved consumption. 27 comprehensive real-life WDNs have been examined by means of the new metric and with the help of complex network theory, exploiting the concept of the degree distribution and topology-based structural properties (e.g. network diameter, clustering coefficient). It was found that metrics based purely on topology suggest different network behaviour as vulnerability analysis, which also includes the hydraulics. The investigation of the global network vulnerabilities has revealed several critically exposed systems, and the local distributions unveiled new properties of WDNs in the case of a random pipe break.

Adhesion force measurements on functionalized microbeads: An in-depth comparison of computer controlled micropipette and fluidic force microscopy.

Characterization of the binding of functionalized microparticles to surfaces with a specific chemistry sheds light on molecular scale interactions. Polymer or protein adsorption are often monitored by colloid particle deposition. Force measurements on microbeads by atomic force microscopy (AFM) or optical tweezers are standard methods in molecular biophysics, but typically have low throughput. Washing and centrifuge assays with (bio)chemically decorated microbeads provide better statistics, but only qualitative results without a calibrated binding force or energy value. In the present work we demonstrate that a computer controlled micropipette (CCMP) is a straightforward and high-throughput alternative to quantify the surface adhesion of functionalized microparticles. However, being an indirect force measurement technique, its in-depth comparison with a direct force measurement is a prerequisite of applications requiring calibrated adhesion force values. To this end, we attached polystyrene microbeads to a solid support by the avidin-biotin linkage. We measured the adhesion strength of the microbeads with both a specialized robotic fluid force microscope (FluidFM BOT) and CCMP. Furthermore, the bead-support contact zone was directly characterized on an optical waveguide biosensor to determine the density of avidin molecules. Distribution of the detachment force recorded on ∼50 individual beads by FluidFM BOT was compared to the adhesion distribution obtained from CCMP measurements on hundreds of individual beads. We found that both methods provide unimodal histograms. We conclude that FluidFM BOT can directly measure the detachment force curve of 50 microbeads in 150 min. CCMP can provide calibrated binding/adhesion force values of 120 microbeads in an hour.

Single cell adhesion assay using computer controlled micropipette.

Cell adhesion is a fundamental phenomenon vital for all multicellular organisms. Recognition of and adhesion to specific macromolecules is a crucial task of leukocytes to initiate the immune response. To gain statistically reliable information of cell adhesion, large numbers of cells should be measured. However, direct measurement of the adhesion force of single cells is still challenging and today's techniques typically have an extremely low throughput (5-10 cells per day). Here, we introduce a computer controlled micropipette mounted onto a normal inverted microscope for probing single cell interactions with specific macromolecules. We calculated the estimated hydrodynamic lifting force acting on target cells by the numerical simulation of the flow at the micropipette tip. The adhesion force of surface attached cells could be accurately probed by repeating the pick-up process with increasing vacuum applied in the pipette positioned above the cell under investigation. Using the introduced methodology hundreds of cells adhered to specific macromolecules were measured one by one in a relatively short period of time (∼30 min). We blocked nonspecific cell adhesion by the protein non-adhesive PLL-g-PEG polymer. We found that human primary monocytes are less adherent to fibrinogen than their in vitro differentiated descendants: macrophages and dendritic cells, the latter producing the highest average adhesion force. Validation of the here introduced method was achieved by the hydrostatic step-pressure micropipette manipulation technique. Additionally the result was reinforced in standard microfluidic shear stress channels. Nevertheless, automated micropipette gave higher sensitivity and less side-effect than the shear stress channel. Using our technique, the probed single cells can be easily picked up and further investigated by other techniques; a definite advantage of the computer controlled micropipette. Our experiments revealed the existence of a sub-population of strongly fibrinogen adherent cells appearing in macrophages and highly represented in dendritic cells, but not observed in monocytes.

Sample handling in surface sensitive chemical and biological sensing: a practical review of basic fluidics and analyte transport.

This paper gives an overview of the advantages and associated caveats of the most common sample handling methods in surface-sensitive chemical and biological sensing. We summarize the basic theoretical and practical considerations one faces when designing and assembling the fluidic part of the sensor devices. The influence of analyte size, the use of closed and flow-through cuvettes, the importance of flow rate, tubing length and diameter, bubble traps, pressure-driven pumping, cuvette dead volumes, and sample injection systems are all discussed. Typical application areas of particular arrangements are also highlighted, such as the monitoring of cellular adhesion, biomolecule adsorption-desorption and ligand-receptor affinity binding. Our work is a practical review in the sense that for every sample handling arrangement considered we present our own experimental data and critically review our experience with the given arrangement. In the experimental part we focus on sample handling in optical waveguide lightmode spectroscopy (OWLS) measurements, but the present study is equally applicable for other biosensing technologies in which an analyte in solution is captured at a surface and its presence is monitored. Explicit attention is given to features that are expected to play an increasingly decisive role in determining the reliability of (bio)chemical sensing measurements, such as analyte transport to the sensor surface; the distorting influence of dead volumes in the fluidic system; and the appropriate sample handling of cell suspensions (e.g. their quasi-simultaneous deposition). At the appropriate places, biological aspects closely related to fluidics (e.g. cellular mechanotransduction, competitive adsorption, blood flow in veins) are also discussed, particularly with regard to their models used in biosensing.