RESUMO
Ultraviolet B (UVB) radiation from the sun may lead to photocarcinogenesis of the skin. Sunscreens were used to protect the skin by reducing UVB irradiance, but sunscreen use did not reduce sunburn episodes. It was shown that UVB-induced erythema depends on surface exposure but not irradiance of UVB. We previously showed that irradiance plays a critical role in UVB-induced cell differentiation. This study investigated the impact of irradiance on UVB-induced photocarcinogenesis. For hairless mice receiving equivalent exposure of UVB radiation, the low irradiance (LI) UVB treated mice showed more rapid tumor development, larger tumor burden, and more keratinocytes harboring mutant p53 in the epidermis as compared to their high irradiance (HI) UVB treated counterpart. Mechanistically, using cell models, we demonstrated that LI UVB radiation allowed more keratinocytes harboring DNA damages to enter cell cycle via ERK-related signaling as compared to its HI UVB counterpart. These results indicated that at equivalent exposure, UVB radiation at LI has higher photocarcinogenic potential as compared to its HI counterpart. Since erythema is the observed sunburn at moderate doses and use of sunscreen was not found to associate with reduced sunburn episodes, the biological significance of sunburn with or without sunscreen use warrants further investigation.
Assuntos
Carcinogênese/efeitos da radiação , Raios Ultravioleta , Adulto , Animais , Bromodesoxiuridina/metabolismo , Butadienos/farmacologia , Carcinogênese/efeitos dos fármacos , Carcinogênese/patologia , Contagem de Células , Sobrevivência Celular/efeitos da radiação , Células Cultivadas , Dano ao DNA , Dermatite de Contato/patologia , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Fase G2/efeitos da radiação , Humanos , Terapia de Imunossupressão , Queratinócitos/efeitos dos fármacos , Queratinócitos/patologia , Queratinócitos/efeitos da radiação , Camundongos Pelados , Mitose/efeitos da radiação , Mutação/genética , Nitrilas/farmacologia , Inibidores de Proteínas Quinases/farmacologia , Dímeros de Pirimidina/metabolismo , Neoplasias Cutâneas/patologia , Proteína Supressora de Tumor p53/metabolismoRESUMO
Hepatitis B virus- (HBV-) associated hepatocellular carcinoma (HCC) is the most common type of liver cancer. However, the underlying mechanism of HCC tumorigenesis is very complicated and HBV-encoded X protein (HBx) has been reported to play the most important role in this process. Activation of downstream signal pathways of epidermal growth factor receptor (EGFR) family is known to mediate HBx-dependent HCC tumor progression. Interestingly, HER2 (also known as ErbB2/Neu/EGFR2) is frequently overexpressed in HBx-expressing HCC patients and is associated with their poor prognosis. However, it remains unclear whether and how HBx regulates HER2 expression. In this study, our data showed that HBx expression increased HER2 protein level via enhancing its mRNA stability. The induction of RNA-binding protein HuR expression by HBx mediated the HER2 mRNA stabilization. Finally, the upregulated HER2 expression promoted the migration ability of HBx-expressing HCC cells. These findings deciphered the molecular mechanism of HBx-mediated HER2 upregulation in HBV-associated HCC.
