RESUMO
BACKGROUND: The appropriate approach for video-assisted thoracoscopic surgery for early stage thymoma remains unclear. The present study aimed to explore the safety and feasibility of subxiphoid and subcostal arch thoracoscopic thymectomy in comparison with unilateral thoracoscopic thymectomy for treatment of early stage thymoma. METHODS: The outcomes of 237 patients without myasthenia gravis who had undergone thoracoscopic thymectomy for Masaoka stage I and II thymoma from January 2015 to May 2019 at our center were retrospectively evaluated (subxiphoid and subcostal arch approach, 39; unilateral video-assisted thoracoscopic surgery approach, 198). A propensity score matching analysis was generated to control for selection bias due to nonrandom group assignment in a 1:1 manner. RESULTS: There was no surgery-related mortality in included patients. Matching of patients according to propensity score resulted in a cohort that consisted of 39 patients in both groups. Patients had similar clinical characteristics in both groups. Compared with patients in the unilateral group, patients in the subxiphoid group yielded lower pain scores at 24 and 72 hours after operation, respectively (P < .01). In addition, the operation time was longer in the subxiphoid group (147.5 ± 43.6 vs 93.2 ± 33.8 minutes, P < .01). There were no significant differences in blood loss, total volume and time of drainage, complications, or postoperative hospital stays between the two groups. CONCLUSIONS: Subxiphoid and subcostal arch thoracoscopic thymectomy for early stage thymoma appears to be a safe and feasible procedure. It is considered to be less invasive as it may cause minimal postoperative pain compared with the unilateral video-assisted thoracoscopic surgery approach.
Assuntos
Timoma , Neoplasias do Timo , Humanos , Pontuação de Propensão , Estudos Retrospectivos , Cirurgia Torácica Vídeoassistida/métodos , Timectomia/métodos , Timoma/cirurgia , Neoplasias do Timo/cirurgiaRESUMO
Previous evidence suggests that long non-coding colon cancer-associated transcript-1(CCAT1) plays a pivotal role in the progression of a variety of tumors. However, little is known about its role in lung adenocarcinoma (LAD). In this study, we found LAD tissue samples had a higher expression of CCAT1 but a lower expression of miR-219-1 compared to their adjacent non-tumor tissues. CCAT1 negatively regulated the expression of miR-219-1. miR-219-1 suppressed the proliferation of A549 and H1299 cells. Knockdown of CCAT1 inhibited the proliferation, migration, and invasion of A549 and H1299 cells, which were reversed by the miR-219-1 inhibitor. CCAT1 knockdown increased the expression of E-cadherin but decreased the expressions of N-cadherin and vimentin, which were restored by the miR-219-1 inhibitor. In vivo, knockdown of CCAT1 suppressed the tumor growth of LAD xenografts, which were rescued by the inhibition of miR-219-1. In summary, our findings suggested that CCAT1 promotes the progression of LAD via sponging miR-219-1, providing a potential therapeutic target for LAD.
RESUMO
2-Keto-d-gluconic acid (2KGA) is an important organic acid derived from d-glucose and is used to produce the food antioxidant erythorbic acid. To improve the 2KGA production performance and cell reusability, various carriers such as calcium alginate, k-carrageenan, chitosan, and poly(vinyl alcohol)-alginate were evaluated to immobilize Pseudomonas plecoglossicida JUIM01 resting cells. Calcium alginate was shown to be a suitable carrier since the immobilized cells had the highest number of reuse times and produced the highest 2KGA concentration of 171.77 g/L, with a productivity of 3.58 g/L·h and conversion ratio of 98.38%. The cell concentration, cultivation temperature, aeration rate and initial glucose concentration were further optimized in a 5-L airlift bioreactor to obtain the best 2KGA production performance by calcium alginate-immobilized P. plecoglossicida cells. Under the optimal conditions including a cell concentration of 4.0 g/L, glucose concentration of 126.0 g/L, temperature of 34 °C and aeration rate of 2.8 L/min, 134.45 g/L 2KGA was produced by alginate-immobilized P. plecoglossicida cells within 30 h, with a total productivity of 4.48 g/L·h and yield of 1.07 g/g (conversion ratio of over 99.0%). The immobilized cells maintained a stable conversion capacity after nine reuses and 25 days of storage at 4 °C, which indicated that calcium alginate immobilization of P. plecoglossicida cells had industrial practicability for 2KGA production.
RESUMO
This study aimed to compare the short and long-term survival outcomes between video-assisted thoracoscopic surgery (VATS) and open resection of lung metastases from colorectal cancer. Between January 2006 and January 2013, 57 patients underwent VATS of lung metastases from colorectal cancer. These patients were compared with a consecutive matched group of 57 patients who underwent open resection within the same period. The two groups were similar in terms of age, gender, tumor size, number of tumors, tumor laterality and type of pulmonary resections. The operative time was longer in the VATS group, but the estimated blood loss was less in the VATS group than in the open group. Postoperative 30-day mortality, 30-day complications were similar between the groups. More complications were classified as major in patients underwent open resection, though the difference was not significant (P = 0.297). The 5-year overall survival rate was 50% for VATS and 46% for open resection (P = 0.251). The 5-year overall disease-free survival time was similar in two groups (P = 0.457). The findings suggest that VATS is associated with less blood loss than open resection for lung metastases of colorectal cancer. According to our results, VATS for lung metastases from colorectal cancer is equivalent to open resection in terms of long-term survival outcomes.
