Factors influencing risk assessments of brominated flame-retardants; evidence based on seafood from the North East Atlantic Ocean.

Brominated flame-retardants (BFRs) such as polybrominated diphenyl ethers (PBDE) and hexabromocyclododecane (HBCD) are considered hazardous to human health. Due to their persistence, they are still present in the environment and in biota and seafood is major contributor of BFRs to human exposure. Here, we used data from >9700 samples of wild and farmed fish, fish feed and fish feed ingredients collected from the North Atlantic between 2006 and 2016 aiming to investigate factors influencing the risk assessments of BFRs. Due to most representative number of analyses, PBDEs were the main focus of investigation. Mean ∑PBDE in fillet samples ranged from below quantification in Atlantic cod fillet to 2.0 µg kg-1 in Atlantic halibut. The main congener contributing to the ∑PBDE in all species was BDE 47. Factors affecting the level of BFR in seafood were multifaceted, and the levels were within species mainly determined by fish age, geographical origin and time of sampling. BDE 47, 99, 153 and HBCD were selected for margin of exposure (MOE) evaluation. When other sources of BFR than seafood were excluded, our risk assessment showed low risk at the current dietary intake of seafood. However, the dietary intake of BDE 99 may be of concern for toddlers when all sources are considered. The choice of fish species, dietary studies, choice of statistics, as well as exposure from other sources than seafood, were all factors that influenced the final MOE of BFRs. We propose the use of regression on order statistics as a tool for risk assessment, to illustrate means and spreads in large surveillance datasets to avoid the issue of measurements below the limit of quantification. A harmonized, updated evaluation of the risk associated with exposure to BFRs from diet, air and dust is warranted, where the fish species most commonly consumed also is taken into consideration.

Contaminant levels in Norwegian farmed Atlantic salmon (Salmo salar) in the 13-year period from 1999 to 2011.

BACKGROUND: Environmental pollutants such as dioxins and PCBs, heavy metals, and organochlorine pesticides are a global threat to food safety. In particular, the aquatic biota can bioaccumulate many of these contaminants potentially making seafood of concern for chronic exposure to humans. OBJECTIVES: The main objective was to evaluate trends of contaminant levels in Norwegian farmed Atlantic salmon in light of the derived tolerable intakes. METHODS: Through an EU-instigated surveillance programme, the Norwegian Food Safety Authority (NFSA) has between 1999 and 2011 collected more than 2300 samples of Norwegian farmed Atlantic salmon (Salmo salar) for contaminant analyses. The fillets of these fish were homogenised and analysed for dioxins, PCBs, heavy metals and organochlorine pesticides. RESULTS: The levels of the contaminants mercury, arsenic, dioxins, dioxin-like PCBs and DDT in Norwegian farmed salmon fillet have decreased during our period of analyses. The levels of cadmium, lead and several organochlorine pesticides were too close to the limit of quantification to calculate time trends. For PCB6 and quantifiable amounts of pesticides, except DDT, stable levels were observed. CONCLUSION: The contaminant levels in Norwegian farmed salmon have generally decreased between 1999 and 2011. Excluding other dietary sources, the levels of dioxins and dioxin-like PCBs in 2011 allowed consumption of up to 1.3kg salmon per week to reach the tolerable weekly intake. The group of contaminants which was the limiting factor for safe consumption of Norwegian farmed salmon, based on currently established TWI values, is the sum of dioxins and dioxin-like PCBs.

An automated extraction approach for isolation of 24 polyaromatic hydrocarbons (PAHs) from various marine matrixes.

An efficient and selective analytical method for determination and quantification of 24 various polyaromatic hydrocarbons (PAHs) in blue mussel (Mytilus edulis), salmon fillet (Salmo salar), fish oil and fish feed has been developed. The samples were extracted by means of accelerated solvent extraction (ASE) technique followed by a purification step with gel permeation chromatography (GPC). Identification and quantification were performed by using GC/MS. The novel combination of silica and alumina in the extraction step furnishes highly purified analytes for the most of the 24 PAHs investigated, and thus a fast and selective analytical method is developed. A small limitation with the method concerns the quantification of acenaphthene (Ace), fluorene (Fl), pyrene (Py) and benzo[a]anthracene (BaA), as the found values for these compounds do not match the certified values (SRM 2977, mussel tissue). Chrysene (Chr) and triphenylene (Tph) give unresolved peaks. The limits of detection (LOD) and limits of quantification (LOQ) found for benzo[a]pyrene (BaP) were 1.7 and 0.44 pg/g (LOD), and 5.8 and 1.5 pg/g (LOQ) for salmon fillet and blue mussel, respectively. This is in a very good accordance with respect to the European Community legislation for official control of BaP levels in foodstuff. The method may be used for qualitative identification of petroleum compounds in marine matrixes.