RESUMO
The influence of N-stearoylethanolamine (NSE) on total 11-hydroxycorticosteroids (11-HCS) and testosterone level in the blood of male rats in normal conditions and under the action of 17beta-estradiol (400 mkg/kg of body weight during 3 days) was studied. It was shown that NSE administration per os (50 mg/kg of body weight during 7 days) to intact animals did not change the level of 11-HCS and of testosterone. The administration of NSE to estrogenized male rats decreased the elevated level of 11-HCS and normalized the amount of testosterone in blood. The correction of alterated weight of adenohypophysis and testis of estrogenized male rats compared to control can be a direct evidence of NSE-mediated modelling of the effect on hypothalamic-pituitary hormone system. The effect of NSE in the testis of estrogenized male rats inhibited the process of lipid peroxidation, caused the decrease of the amount of thiobarbituric acid reactive substances and increased the activity of superoxide dismutase and catalase. The NSE showed more expressed antioxidative effect compared to vitamin E. Taking into consideration all above mentioned data we suggested that NSE administration to male rats protected Leydig cells from damage under the increase of estrogen level.
Assuntos
Antioxidantes/farmacologia , Estradiol/efeitos adversos , Etanolaminas/farmacologia , Modelos Biológicos , Ácidos Esteáricos/farmacologia , Testículo/efeitos dos fármacos , 11-Hidroxicorticosteroides/sangue , Animais , Células Intersticiais do Testículo/efeitos dos fármacos , Células Intersticiais do Testículo/metabolismo , Peroxidação de Lipídeos/efeitos dos fármacos , Masculino , Ratos , Testículo/metabolismo , Testosterona/sangueRESUMO
The effect of 17beta-estradiol on the change of proapoptotic protein Bax mRNA level and DNA laddering in human adrenal cortex tissue was studied. The adding of 17beta-estradiol to incubation medium caused a decrease of the proapoptotic protein Bax mRNA level in adrenocorticocytes by 22% in comparison with control. Hormone influence decreases the DNA fragmentation by 50%. The obtained data suggest that 17beta-estradiol caused a significant antiapoptotic effect in human adrenal cortex.
Assuntos
Córtex Suprarrenal/efeitos dos fármacos , Apoptose/efeitos dos fármacos , Fragmentação do DNA/efeitos dos fármacos , Estradiol/farmacologia , Estrogênios/farmacologia , RNA Mensageiro/biossíntese , Proteína X Associada a bcl-2/biossíntese , Córtex Suprarrenal/metabolismo , Eletroforese em Gel de Ágar , Humanos , Reação em Cadeia da Polimerase , Técnicas de Cultura de TecidosRESUMO
Estradiol treatment produced significant increase of total 11-hydroxycorticosteroids level in the blood of intact and castrated rats. Activity of protein kinase A increased in the cytosol and membrane fraction of adrenocorticocytes of intact and orchiectomized rats after estradiol influence. Activity of protein kinase C significantly raised in the cytosol and membrane fraction of adrenocortical cells in all investigated groups. Our results suggest that cAMP-dependent protein kinase A and protein kinase C mediate estradiol effects in adrenal cortex.
