RESUMO
Alveolar epithelial type II (AT II) cells are essential for lung development and remodeling, as they are precursors for type I cells and also produce other non-repair cells (fibroblasts). Progenitor cells are believed to possess capability of multi-potent transdifferentiation, which is closely related to the niche, suggesting the importance of establishment of a lung progenitor cell niche model. We hypothesized that pulmonary surfactant-associated protein A (SPA) suicide gene system would cause AT II cell to kill itself through apoptosis and leave its niche. In vitro, the recombinant adeno-associated virus vectors-SPA-thymidine kinase (rAAV-SPA-TK) system was established to get targeted apoptotic AT II cells. The apoptosis of AT II cells was detected by using MTT. The results showed that cloned SPA gene promoter had specific transcriptional activity in SPA high expression cells, and SPA high expression cells (H441) transfected with TK gene had higher sensitivity to ganciclovir (GCV) than SPA low expression cells (A549). In vivo, increased apoptosis of AT II cells induced by GCV in rAAV-SPA-TK system was observed by TUNEL. Finally, the successful packaging and application of rAAV-SPA-TK system provide experimental basis to get specific lung progenitor cell (AT II) niche in vitro and in vivo.
Assuntos
Humanos , Antivirais , Farmacologia , Apoptose , Linhagem Celular Tumoral , Sobrevivência Celular , Células Cultivadas , Dependovirus , Genética , Relação Dose-Resposta a Droga , Eletroforese em Gel de Poliacrilamida , Células Epiteliais , Biologia Celular , Metabolismo , Ganciclovir , Farmacologia , Regulação Neoplásica da Expressão Gênica , Genes Transgênicos Suicidas , Genética , Vetores Genéticos , Genética , Marcação In Situ das Extremidades Cortadas , Luciferases , Genética , Metabolismo , Regiões Promotoras Genéticas , Genética , Alvéolos Pulmonares , Biologia Celular , Metabolismo , Proteína A Associada a Surfactante Pulmonar , Genética , Metabolismo , Timidina Quinase , Genética , MetabolismoRESUMO
Alveolar epithelial type II (AT II) cells are essential for lung development and remodeling, as they are precursors for type I cells and also produce other non-repair cells (fibroblasts). Progenitor cells are believed to possess capability of multi-potent transdifferentiation, which is closely related to the niche, suggesting the importance of establishment of a lung progenitor cell niche model. We hypothesized that pulmonary surfactant-associated protein A (SPA) suicide gene system would cause AT II cell to kill itself through apoptosis and leave its niche. In vitro, the recombinant adeno-associated virus vectors-SPA-thymidine kinase (rAAV-SPA-TK) system was established to get targeted apoptotic AT II cells. The apoptosis of AT II cells was detected by using MTT. The results showed that cloned SPA gene promoter had specific transcriptional activity in SPA high expression cells, and SPA high expression cells (H441) transfected with TK gene had higher sensitivity to ganciclovir (GCV) than SPA low expression cells (A549). In vivo, increased apoptosis of AT II cells induced by GCV in rAAV-SPA-TK system was observed by TUNEL. Finally, the successful packaging and application of rAAV-SPA-TK system provide experimental basis to get specific lung progenitor cell (AT II) niche in vitro and in vivo.
RESUMO
Objective To analyze the distribution features of stroke types of patients in Changde city to provide a theoretical basis for stroke prevention.Methods A total of 3594 first-onset patients from 5338 patients with stroke met the standards of Changde city center stroke registry program,chosen from 2011 January to December 2011,were used as the research object.According to the age of the patients,they were divided into 7 groups (group of 18-29 years old,group of 30-39 years old,group of 40-49 years old,group of 50-59 years old,group of 60-69 years old,group of 70-79 years old,group of elder than 80 years old); the gender ratio,incident of disease,cerebral infarction types,responsibility vascular distributions of ischemic stroke,and hemorrhagic stroke patterns of these patients were analyzed.Results Hemorrhagic stroke was noted in 864 patients (24％) and ischemic stroke in 2730 patients (76％).No significant difference on gender was noted in hemorrhagic stroke patients and ischemic stroke patients of different age groups (P＞0.05); the incident of stroke in patients of different age groups was almost the same,with an high peak level of patients elder than 60 years old.lacunar infarction and big vasogenic cerebral infarction had higher risk of incidence than other types.Patients of 40-80 years old had the highest rate of these two stokes,while other types of stoke preferred young age person.The responsible vessels were mainly located in the arterial system.Basal ganglia hemorrhage was the main type of patients of each age group; patients of 30-60 years old mainly had subarachnoid hemorrhage and patients of 40-80 years old mainly had cerebral hemorrhage.Conclusion The ischemic stroke and hemorrhagic stroke types in Changde city are basically the same as the existing research at home and abroad; single cerebral stroke risk factors may not be the same,which needs to be further studied