Discovery of N-(1,4-Benzoxazin-3-one) urea analogs as Mode-Selective TRPV1 antagonists.

A series of 1,4-benzoxazin-3-one analogs were investigated to discover mode-selective TRPV1 antagonists, since such antagonists are predicted to minimize target-based adverse effects. Using the high-affinity antagonist 2 as the lead structure, the structure activity relationship was studied by modifying the A-region through incorporation of a polar side chain on the benzoxazine and then by changing the C-region with a variety of substituted pyridine, pyrazole and thiazole moieties. The t-butyl pyrazole and thiazole C-region analogs provided high potency as well as mode-selectivity. Among them, antagonist 36 displayed potent and capsaicin-selective antagonism with IC50 = 2.31 nM for blocking capsaicin activation and only 47.5 % inhibition at 3 µM concentration toward proton activation, indicating that more than a 1000-fold higher concentration of 36 was required to inhibit proton activation than was required to inhibit capsaicin activation. The molecular modeling study of 36 with our homology model indicated that two π-π interactions with the Tyr511 and Phe591 residues by the A- and C-region and hydrogen bonding with the Thr550 residue by the B-region were critical for maintaining balanced and stable binding. Systemic optimization of antagonist 2, which has high-affinity but full antagonism for activators of all modes, led to the mode-selective antagonist 36 which represents a promising step in the development of clinical TRPV1 antagonists minimizing side effects such as hyperthermia and impaired heat sensation.

Discovery of N-(1-(2-hydroxyethyl)quinolin-2-one)-N'-(1-phenyl-1H-pyrazol-5-yl)methyl) urea as Mode-Selective TRPV1 antagonist.

To discover mode-selective TRPV1 antagonists as thermoneutral drug candidates, the previous potent antagonist benzopyridone 2 was optimized based on the pharmacophore A- and C-regions. The structure activity relationship was investigated systematically by modifying the A-region by incorporating a polar side chain on the pyridone and then by changing the C-region with a variety of substituted pyridine and pyrazole moieties. The 3-t-butyl and 3-(1-methylcyclopropyl) pyrazole C-region analogs provided high potency as well as mode-selectivity. Among them, 51 and 54 displayed potent and capsaicin-selective antagonism with IC50 = 2.85 and 3.27 nM to capsaicin activation and 28.5 and 31.5 % inhibition at 3 µM concentration toward proton activation, respectively. The molecular modeling study of 51 with our homology model indicated that the hydroxyethyl side chain in the A-region interacted with Arg557 and Glu570, the urea B-region engaged in hydrogen bonding with Tyr511 and Thr550, respectively, and the pyrazole C-region made two hydrophobic interactions with the receptor. Optimization of antagonist 2, which has full antagonism for activators of all modes, lead to mode-selective antagonists 51 and 54. These observations will provide insight into the future development of clinical TRPV1 antagonists without target-based side effects.

Interface engineering in a nitrogen-rich COF/BiOBr S-scheme heterojunction triggering efficient photocatalytic degradation of tetracycline antibiotics.

Tetracycline (TC) antibiotics, extensively utilized in livestock farming and aquaculture, pose significant environmental challenges. Photocatalysis, leveraging renewable sunlight and reusable photocatalysts, offers a promising avenue for mitigating TC pollution. However, identifying robust photocatalysts remains a formidable challenge. This study introduces a novel hollow-flower-ball-like nanoheterojunction composed of a nitrogen-rich covalent organic framework (N-COF) coupled with BiOBr (BOB), a semiconductor with a higher Fermi level. The synthesized N-COF/BOB S-scheme nanoheterojunction features an expanded contact interface, strengthened chemical bonding, and unique band topologies. The N-COF/BOB composites showcased exceptional TC degradation performance, achieving an 81.2% removal of 60 mg/L TC within 2 h, markedly surpassing the individual efficiencies of N-COF and BOB by factors of 3.80 and 5.96, respectively. Furthermore, the total organic carbon (TOC) removal efficiency highlights a superior mineralization capacity in the N-COF/BOB composite compared to the individual components, N-COF and BOB. The toxicity assessment revealed that the degradation intermediates possess diminished environmental toxicity. This enhanced performance is ascribed to the robust S-scheme nanoheterojunction structure, which promotes efficient photoinduced electron transfer from BOB to N-COF. This process also augments the separation of photogenerated charge carriers, resulting in an increased yield of superoxide radicals (∙O2-) and hydroxyl radicals (∙OH). These reactive species significantly contribute to the degradation and mineralization of TC. Consequently, this study introduces a sustainable approach for addressing emerging antibiotic contaminants, employing COF-based photocatalysts.

Profiling of Serum miRNAs Constructs a Diagnostic 3-miRNA Panel for Clear-Cell Renal Cell Carcinoma.

BACKGROUND: Renal cell carcinoma (RCC) carries significant morbidity and mortality globally with an increasing incidence per year predominantly represented by clear-cell renal cell carcinoma (ccRCC) which accounts for 70-80% of all RCC cases. MicroRNAs(miRNAs) implicate tumor development and progression in epigenetic mechanisms and available profiling of serum miRNAs potentiate them as diagnostic markers for various cancers. MATERIALS AND METHODS: A total of 108 ccRCC patients and 112 normal controls were enrolled. A 3-stage experiment was conducted to identify differentially expressed serum miRNAs in ccRCC and establish a diagnostic miRNAs panel. Additionally, bioinformatic analysis was employed to predict selected miRNAs' target genes, preform functional annotation and explore the roles in ccRCC. RESULTS: MiR-429, miR-10a-5p, miR-154-5p were found to be up-regulated miRNAs. Inversely, miR-27a-3p and miR-221-3p were found to be down-regulated miRNAs. These 5 miRNAs were selected to construct diagnostic panel by backward stepwise logistic regression analysis and ultimately a 3-miRNA panel (miR-429, miR-10a-5p and miR-27a-3p) was established [area under the curve (AUC) = 0.897, sensitivity = 85.0%, specificity = 83.3%]. CONCLUSION: The panel of 3-miRNA holds promise as a novel, convenient, and noninvasive diagnostic method for early detection of ccRCC.

Disparities in prostate cancer screening, diagnoses, management, and outcomes between Indigenous and non-Indigenous men in a universal health care system.

BACKGROUND: Indigenous Peoples have higher morbidity rates and lower life expectancies than non-Indigenous Canadians. Identification of disparities between Indigenous and non-Indigenous men regarding prostate cancer (PCa) screening, diagnoses, management, and outcomes was sought. METHODS: An observational cohort of men diagnosed with PCa between June 2014 and October 2022 was studied. Men were prospectively enrolled in the province-wide Alberta Prostate Cancer Research Initiative. The primary outcomes were tumor characteristics (stage, grade, and prostate-specific antigen [PSA]) at diagnosis. Secondary outcomes were PSA testing rates, time from diagnosis to treatment, treatment modality, and metastasis-free, cancer-specific, and overall survivals. RESULTS: Examination of 1,444,974 men for whom aggregate PSA testing data were available was performed. Men in Indigenous communities were less likely to have PSA testing performed than men outside of Indigenous communities (32 vs. 46 PSA tests per 100 men [aged 50-70 years] within 1 year; p < .001). Among 6049 men diagnosed with PCa, Indigenous men had higher risk disease characteristics: a higher proportion of Indigenous men had PSA ≥ 10 ng/mL (48% vs. 30%; p < .01), TNM stage ≥ T2 (65% vs. 47%; p < .01), and Gleason grade group ≥ 2 (79% vs. 64%; p < .01) compared to non-Indigenous men. With a median follow-up of 40 months (interquartile range, 25-65 months), Indigenous men were at higher risk of developing PCa metastases (hazard ratio, 2.3; 95% CI, 1.2-4.2; p < .01) than non-Indigenous men. CONCLUSIONS: Despite receiving care in a universal health care system, Indigenous men were less likely to receive PSA testing and more likely to be diagnosed with aggressive tumors and develop PCa metastases than non-Indigenous men.

Syntheses and antitumor activities of neorautenol and shinpterocarpin analogs.

The natural products neorautenol and shinpterocarpin and their structural analogs were investigated as novel anticancer agents. Twenty-four analogs, including analogs containing a polar chain and simplified analogs, were synthesized efficiently by a modified method from previous reports. The antitumor screening of synthesized compounds toward six cancer cell lines indicated that compounds 37, 42 and 43 with a dialkylaminoethyl-type side chain exhibited more promising activity than neorautenol and shinpterocarpin against lung and colon cancer lines with a range of 4-9 µM. They showed selective toxicity in normal cells.

Build-in electric field in CuWO4/covalent organic frameworks S-scheme photocatalysts steer boosting charge transfer for photocatalytic CO2 reduction.

Covalent organic frameworks (COFs) are crystalline porous materials with enormous potential for realizing solar-driven CO2-to-fuel conversion, yet the sluggish transfer/separation of photoinduced electrons and holes remains a compelling challenge. Herein, a step (S)-scheme heterojunction photocatalyst (CuWO4-COF) was rationally fabricated by a thermal annealing method for boosting CO2 conversion to CO. The optimal CuWO4/COF composite sample, integrating 10 wt% CuWO4 with an olefin (C═C) linked COF (TTCOF), achieved a remarkable gas-solid phase CO yield as high as 7.17 ± 0.35 µmol g-1h-1 under visible light irradiation, which was significantly higher than the pure COF (1.6 ± 0.29 µmol g-1h-1). The enhanced CO2 conversion rate could be attributable to the interface engineering effect and the formation of internal electric field (IEF) directing from TTCOF to CuWO4 according to the theoretical calculation and experimental results, which also proves the electrons transfer from TTCOF to CuWO4 upon hybridization. In addition, driven by the IEF, the photoinduced electrons can be steered from CuWO4 to TTCOF under visible light irradiation as well-elucidated by in-situ irradiated X-ray photoelectron spectroscopy, verifying the S-scheme charge transfer pathway over CuWO4/COF composite heterojunctions, which greatly foster the photoreduction activity of CO2. The preparation technique of the S-scheme heterojunction photocatalyst in this study provides a paradigmatic protocol for photocatalytic solar fuel generation.

Interspersed Bi Promoting Hot Electron Transfer of Covalent Organic Frameworks Boosts Nitrogen Reduction to ammonia.

Seeking highly-efficient, non-pollutant, and chemically robust photocatalysts for visible-light-driven ammonia production still remained challenging, especially in pure water. The key bottle-necks closely correlate to the nitrogen activation, water oxidization, and hydrogen evolution reaction (HER) processes. In this study, a novel Bi decorated imine-linked COF-TaTp (Bi/COF-TaTp) through N-Bi-O coordination is reasonably designed to achieve a boosting solar-to-ammonia conversion of 61 µmol-1  g-1  h-1 in the sacrificial-free system. On basis of serial characterizations and DFT calculations, the incorporated Bi is conducive to the acceleration of charge carriers transfer and N2 activation through the donation and back-donation mode. The N2 adsorption energy of 5% Bi/COF-TaTp is calculated to be -0.19 eV in comparison with -0.09 eV of the pure COF-TaTp and the electron exchange between N2 and the modified catalyst is much more intensive. Moreover, the accompanied hydrogen production process is effectively inhibited by Bi modification, demonstrated by the higher energy barrier for HER over Bi/COF-TaTp (2.62 eV) than the pure COF-TaTp (2.31 eV) when using H binding free energy (ΔGH* ) as a descriptor. This work supplies novel insights for the design of photocatalysts for N2 reduction and intensifies the understanding of N2 adsorption and activation over covalent organic frameworks-based materials.

Interfacial engineering of novel inorganic-organic ß-Ga2O3/COF heterojunction for accelerated charge transfer towards artificial photosynthesis.

Semiconductor-based solar-driven CO2 to fuels has been widely reckoned as an ingenious approach to tackle energy crisis and climate change simultaneously. However, the high carrier recombination rate of the photocatalyst severely dampens their photocatalytic uses. Herein, an inorganic-organic heterojunction was constructed by in-situ growing a dioxin-linked covalent organic framework (COF) on the surface of rod-shaped ß-Ga2O3 for solar-driven CO2 to fuel. This novel heterojunction is featured with an ultra-narrow bandgap COF-318 (absorption edge = 760 nm), which is beneficial for fully utilizing the visible light spectrum, and a wide bandgap ß-Ga2O3 (absorption edge = 280 nm) to directional conduct electrons from COF to reduce CO2 without electron-hole recombination occurred. Results showed that the solar to fuels performance over ß-Ga2O3/COF was much superb than that of COF. The optimized Ga2O3/COF achieved an outstanding CO evolution rate of 85.8 µmol h-1·g-1 without the need of any sacrificial agent or cocatalyst, which was 15.6 times more efficient than COF. Moreover, the analyses of photoluminescence electrochemical characterizations and density functional theory (DFT) calculations revealed that the fascinate construction of ß-Ga2O3/COF heterojunction significantly favored charge separation and the directional transfer of photogenerated electrons from COF to ß-Ga2O3 followed by CO2. This study paves the way for developing effective COF-based semiconductor photocatalysts for solar-to-fuel conversion.

Insights into Photocatalytic Degradation Pathways and Mechanism of Tetracycline by an Efficient Z-Scheme NiFe-LDH/CTF-1 Heterojunction.

Photocatalysis offers a sustainable approach for recalcitrant organic pollutants degradation, yet it is still challenging to seek robust photocatalysts for application purposes. Herein, a novel NiFe layered double hydroxide (LDH)/covalent triazine framework (CTF-1) Z-scheme heterojunction photocatalyst was rationally designed for antibiotics degradation under visible light irradiation. The NiFe-LDH/CTF-1 nanocomposites were readily obtained via in situ loading of NiFe-LDH on CTF-1 through covalent linking. The abundant coupling interfaces between two semiconductor counterparts lay the foundation for the formation of Z-scheme heterostructure, thereby effectively promoting the transfer of photogenerated electrons, inhibiting the recombination of carriers, as well as conferring the nanocomposites with stronger redox ability. Consequently, the optimal photocatalytic activity of the LDH/CTF heterojunction was significantly boosted for the degradation of a typical antibiotic, tetracycline (TC). Additionally, the photodegradation process and the mineralization of TC were further elucidated. These results envision that the LDH/CTF-1 can be a viable photocatalyst for long-term and sustainable wastewater treatment.

Interlayer Charge Transfer Over Graphitized Carbon Nitride Enabling Highly-Efficient Photocatalytic Nitrogen Fixation.

Exploiting cost-effective, high-efficiency, and contamination-free semiconductors for photocatalytic nitrogen reduction reaction (N2 RR) is still a great challenge, especially in sacrificial-free system. On basis of the electron "acceptance-donation" concept, a boron-doped and carbon-deficient g-C3 N4 (Bx CvN) is herein developed through precise dopant and defect engineering. The optimized B15 CvN exhibisted an NH3 production rate of 135.3 µmol h-1  g-1 in pure water with nine-fold enhancement to the pristine graphitic carbon nitride (g-C3 N4 ), on account of the markedly elevated visible-light harvesting, N2 activation, and multi-directional photoinduced carriers transfer. The decorated B atoms with coexistent occupied and empty sp3 hybridized orbitals are theoretically proved to be in charge of the increase of N2 adsorption energy from -0.08 to -0.26 eV and the change in N2 adsorption model from one-way to two-way end-on pattern. Noticeably, the elaborate coordination of doped B atoms and carbon vacancies greatly facilitated the interlayer interaction and vertical charge migration of Bx CvN, which is distinctly revealed through the charge density difference calculations. The current study provides an alternative groundbreaking perspective for advancing photocatalytic N2 RR through the targeted configuration of the defect and dopant sites.

Bladder cancer diagnosis with a four-miRNA panel in serum.

Background: Bladder cancer is one of the most prevalent malignancies. Due to the disadvantage of existing bladder cancer diagnostic tools, miRNAs hold promise as new diagnostic markers. Materials & methods: A total of 224 participants were involved in this three-cohort trial. A total of 15 candidate miRNAs were selected, and miRNAs with diagnostic ability were screened out with quantitative reverse transcription PCR. Diagnostic capability was ascertained by the receiver operating characteristic curve and area under the curve. Bioinformatics analysis was constructed for target gene prediction and functional annotation. Results: Six candidate miRNAs showed significantly different expression between bladder cancer patients and normal controls, and the final diagnostic panel comprised miR-181b-5p, miR-183-5p, miR-199-5p and miR-221-3p. Conclusion: This four-miRNA panel could represent a stable biomarker for bladder cancer diagnosis.

Bladder cancer is one of the most prevalent malignancies. Due to the disadvantage of existing bladder cancer diagnostic tools, miRNAs hold promise as new diagnostic markers. After an experiment composed of 224 participants, the authors screened out six candidate miRNAs that may contribute to diagnosing bladder cancer. The authors also repeatedly verified the reliability of candidate miRNAs. Finally, a combination of multiple miRNAs, consisting of miR-181b-5p, miR-183-5p, miR-199-5p, and miR-221-3p, was better and more reliable in predicting bladder cancer occurrence.

A Four-MicroRNA Panel in Serum as a Potential Biomarker for Screening Renal Cell Carcinoma.

Background: Renal cell carcinoma (RCC) has been a major health problem and is one of the most malignant tumors around the world. Serum microRNA (miRNA) profiles previously have been reported as non-invasive biomarkers in cancer screening. The aim of this study was to explore serum miRNAs as potential biomarkers for screening RCC. Methods: A three-phase study was conducted to explore serum miRNAs as potential biomarkers for screening RCC. In the screening phase, 12 candidate miRNAs related to RCC were selected for further study by the ENCORI database with 517 RCC patients and 71 NCs. A total of 220 participants [108 RCC patients and 112 normal controls (NCs)] were enrolled for training and validation. The dysregulated candidate miRNAs were further confirmed with 30 RCC patients and 30 NCs in the training phase and with 78 RCC patients and 82 NCs in the validation phase. Receiver operating characteristic (ROC) curves and the area under the ROC curve (AUC) were used for assessing the diagnostic value of miRNAs. Bioinformatic analysis and survival analysis were also included in our study. Results: Compared to NCs, six miRNAs (miR-18a-5p, miR-138-5p, miR-141-3p, miR-181b-5p, miR-200a-3p, and miR-363-3p) in serum were significantly dysregulated in RCC patients. A four-miRNA panel was built by combining these candidate miRNAs to improve the diagnostic value with AUC = 0.908. ABCG1 and RNASET2, considered potential target genes of the four-miRNA panel, may play a significant role in the development of RCC. Conclusion: A four-miRNA panel in serum was identified for RCC screening in our study. The four--miRNA panel has a great potential to be a non-invasive biomarker for RCC screening.

A four-miRNA signature in serum as a biomarker for bladder cancer diagnosis.

BACKGROUND: Urinary bladder cancer (BCa) is globally the 10th most frequent cancer. As a novel diagnostic tool, miRNA in serum screening is non-invasive. This project aimed to determine particular serum miRNAs as novel biomarkers for diagnosing urinary BCa. METHODS: We designed a three-phase study with 122 healthy controls (HCs) and 132 BCa patients. The 30 miRNAs' expressions in serum from HCs and BCa patients were detected during the screening phase. The miRNAs with the most dysregulation were tested in the training (HCs vs. BCa, 30 each) and validation (80 HCs vs. 82 BCa) phase further. The diagnostic ability of these candidate miRNAs was estimated by the receiver operating characteristic (ROC) curves as well as the area under the ROC curve (AUC). The miRNAs' target genes and their annotations to functions were predicted utilizing bioinformatic assays. RESULTS: Six serum miRNAs (miR-124-3p, miR-182-5p, miR-1-3p, miR-196a-5p, miR-23b-3p and miR-34a-5p) had significantly different expression between BCa patients and HCs in the training and validation phase. The four-microRNA panel improved the diagnostic value, with AUC =0.985. The result of bioinformatic analysis showed that these miRNAs' target genes in the panel may be related to the MAPK signaling pathway in bladder cancer. CONCLUSIONS: Our study identified a four-miRNA panel that is a non-invasive new biomarker for diagnosing BCa.

Cobalt quantum dots as electron collectors in ultra-narrow bandgap dioxin linked covalent organic frameworks for boosting photocatalytic solar-to-fuel conversion.

Photocatalysis offers a sustainable paradigm for solar-to-fuel conversion because it conflates the merits of renewable solar energy and reusable catalysts. However, the seek for robust photocatalysts that can utilize the full visible light spectrum remains challenging. Herein, cobalt quantum dots (Co QDs) were integrated into ultra-narrow bandgap dioxin linked covalent organic frameworks (COF-318) for photocatalytic solar-to-fuel conversion under full spectrum of visible light irradiation. The optimal Co10-COF exhibited superior photocatalytic CO2 reduction performance, affording a CO yield of 4232 µmol∙g-1∙h-1 and H2 evolution of 6611 µmol∙g-1∙h-1. Specifically, Co QDs played a crucial role in boosting the photocatalytic performance, which acted as electron collectors to capture the photoinduced electrons and then conveyed them to CO2 molecules. Moreover, the Co QDs modification significantly improved the CO2 adsorption and activation capacity, as well as prolonging the lifetime of photogenerated carriers. This work reveals an operable pathway for fabricating promising photocatalyst for visible-light-driven solar-to-fuel generation and provides insight into the impact of the integration of Co QDs on COF-based photocatalysts.

Purifying water with silver nanoparticles (AgNPs)-incorporated membranes: Recent advancements and critical challenges.

In the face of the growing global water crisis, membrane technology is a promising means of purifying water and wastewater. Silver nanoparticles (AgNPs) have been widely used to improve membrane performance, for antibiofouling, and to aid in photocatalytic degradation, thermal response, and electro-conductivity. However, several critical issues such as short antimicrobial periods, trade-off effects and silver inactivation seriously restrict the engineering application of AgNPs-incorporated membranes. In addition, there is controversy around the use of AgNPs given the toxic preparation process and environmental/biological risks. Hence, it is of great significance to summarize and analyze the recent developments and critical challenges in the use of AgNPs-incorporated membranes in water and wastewater treatment, and to propose potential solutions. We reviewed the different properties and functions of AgNPs and their corresponding applications in AgNPs-incorporated membranes. Recently, multifunctional, novel AgNP-incorporated membranes combined with other functional materials have been developed with high-performance. We further clarified the synergistic mechanisms between AgNPs and these novel nanomaterials and/or polymers, and elucidated their functions and roles in membrane separation. Finally, the critical challenges of AgNPs-incorporated membranes and the proposed solutions were outlined: i) Prolonging the antimicrobial cycle through long-term and controlled AgNPs release; ii) Overcoming the trade-off effect and organic fouling of the AgNPs-incorporated membranes; iii) Preparation of sustainable AgNPs-incorporated membranes; iv) Addressing biotoxicity induced by AgNPs; and v) Deactivation of AgNPs-incorporated membrane. Overall, this review provides a comprehensive discussion of the advancements and challenges of AgNPs-incorporated membranes and guides the development of more robust, multi-functional and sustainable AgNPs-incorporated membranes.

Challenges in quantifying and characterizing dissolved organic carbon: Sampling, isolation, storage, and analysis.

Despite the advancements in analytical techniques, there are still great challenges and difficulties in accurately and effectively quantifying and characterizing dissolved organic carbon (DOC) in environmental samples. The objectives of this review paper are (a) to understand the roles and variability of DOC along the water continuum; (b) to identify the constraints, inconsistences, limitations, and artifacts in DOC characterization; and (c) to provide recommendations and remarks to improve the analytical accuracy. For the first objective, we summarize the four ecological and engineering roles of DOC along the water continuum from source water to municipal utility, including nutrients and energy sources, controlling the fates of micropollutants, buffering capacity, and treatability and precursors of disinfection byproducts. We also discuss three major challenges in DOC analysis, including spatial and temporal variations, degradability and stability, and unknown structures and formulas. For the second objective, we review the procedures and steps in DOC analysis, including sampling in diverse environmental matrices, isolation of DOC fraction, storage and preservation techniques, and analyses on bulk chemical characteristics. We list and discuss the available options and evaluate the advantages and disadvantages of each choice. Last, we provide recommendations and remarks for each stage: sampling, isolation, storage, and analysis.

An Artificial Photosystem of Metal-Insulator-CTF Nanoarchitectures for Highly Efficient and Selective CO2 Conversion to CO.

It is of pivotal significance to explore robust photocatalysts to promote the photoreduction of CO2 into solar fuels. Herein, an intelligent metal-insulator-semiconductor (MIS) nano-architectural photosystem was constructed by electrostatic self-assembly between cetyltrimethylammonium bromide (CTAB) insulator-capped metal Ni nanoparticles (NPs) and covalent triazine-based frameworks (CTF-1). The metal-insulator-CTF composites unveiled a substantially higher CO evolution rate (1254.15 µmol g-1 h-1 ) compared with primitive CTF-1 (1.08 µmol g-1 h-1 ) and reached considerable selectivity (98.9 %) under visible-light irradiation. The superior photocatalytic CO2 conversion activity over Ni-CTAB-CTF nanoarchitecture could be attributed to the larger surface area, reinforced visible-light response, and CO2 capture capacity. More importantly, the Ni-CTAB-CTF nanoarchitecture endowed the photoexcited electrons on CTF-1 with the ability to tunnel across the thin CTAB insulating layer, directionally migrating to Ni NPs and thereby leading to the efficient separation of photogenerated electrons and holes in the photosystem. In addition, isotope-labeled (13 CO2 ) tracer results verified that the reduction products come from CO2 rather than the decomposition of the photocatalysts. This study opens a new avenue for establishing a highly efficient and selective artificial photosystem for CO2 conversion.

Identification of a three-miRNA panel in serum for bladder cancer diagnosis by a diagnostic test.

Background: Bladder cancer (BC) is the tenth most common cancer in the world. Serum microRNA (miRNA) profiles previously have been reported as non-invasive biomarkers in cancer screening. The non-invasive and reliable diagnostic biomarkers are urgently needed for detecting BC, while cystoscopy is invasive. Our study aimed to identify candidate miRNAs in serum as potential diagnostic biomarkers for BC detection. Methods: This study was including the screening stage, training stage, and validation stage with 137 BC patients and 127 healthy controls (HCs). We identified the expression of 28 serum miRNAs from 5 BC pools and 3 HC pools in the initial screening stage. The other 112 BC patients and 112 HCs were randomly divided into training stage with 30 BC patients and 30 HCs and validation stages with 82 BC patients and 82 HCs. These HCs matched BC patients based on age and gender with P value >0.05. Identified dysregulated miRNAs were further confirmed in the training stage, and validation stages by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The diagnostic value of miRNAs was assessed by receiver operating characteristic (ROC) curves and the area under the ROC curve (AUC). Target genes of 3 candidate miRNAs were predicted by bioinformatic analysis. Results: Five miRNAs (miR-106a-5p, miR-145-5p, miR-132-3p, miR-7-5p and miR-148b-3p) in serum were obviously dysregulated in BC patients compared to HCs. The ability to diagnose BC of 3 candidate miRNAs was estimated by AUC, with miR-132-3p (AUC =0.781; sensitivity =68.29%, specificity =81.71%), miR-7-5p (AUC =0.778; sensitivity =59.76%, specificity =84.15%) and miR-148b-3p (AUC =0.837; sensitivity =81.71%, specificity =71.95%). Combined application of these candidate miRNAs with parallel test could improve the diagnostic value (AUC =0.922; sensitivity =90.24%, specificity =81.71%). BNC2, GAS7, and NTRK2, considered as target genes of the three-miRNA panel, may play an important role in the process of BC development. Conclusions: A three-miRNA panel in serum was identified for BC diagnosis in our study, which HCs were used for differential diagnosis. The three-miRNA panel (miR-132-3p, miR-7-5p, and miR-148b-3p) might be performed as a non-invasive and convenient diagnostic tool for BC screening and diagnosis.

Rational Design of Novel COF/MOF S-Scheme Heterojunction Photocatalyst for Boosting CO2 Reduction at Gas-Solid Interface.

Solar-driven photoreduction of CO2 into valuable fuels offers a sustainable technology to relieve the energy crisis as well as the greenhouse effect. Yet the exploration of highly efficient, selective, stable, and environmental benign photocatalysts for CO2 reduction remains a major issue and challenge. The interfacial engineering of heterojunction photocatalysts could be a valid approach to boost the efficiency of the catalytic process. Herein, we propose a novel covalent organic framework/metal organic framework (COF/MOF) heterojunction photocatalyst, using olefin (C═C) linked covalent organic framework (TTCOF) and NH2-UiO-66 (Zr) (NUZ) as representative building blocks, for enhanced CO2 reduction to CO. The optimized TTCOF/NUZ exhibited a superior CO yield (6.56 µmol g-1 h-1) in gas-solid system when irradiated by visible light and only with H2O (g) as weak reductant, and it was 4.4 and 5 times higher than pristine TTCOF and NUZ, respectively. The photogenerated electrons transfer route was proposed to follow the typical step-scheme (S-scheme), which was affirmed by XPS, in situ XPS and EPR characterizations. The boosting CO2 photoreduction activity could be credited to the special charge carrier separation in S-scheme heterojunction, which can accelerate photogenerated electrons transportation and improve the redox ability at the interface. This work paves the way for the design and preparation of novel COF/MOF S-scheme heterostructure photocatalysts for CO2 reduction.