RESUMO
The hysteresis of keratinocyte (KC) reepithelialization is an important factor resulting in chronic wounds; however, the molecular mechanisms involved in this cellular response remain yet to be completely elucidated. The present study demonstrated the function of transcription factor Forkhead box O3a (FoxO3a) in KC growth and migration functional effects, resulting in restrained KC reepithelialization during wound healing. In chronic wound tissue samples, the expression of FoxO3a was significantly increased when compared with the acute wound healing group (P<0.01). Overexpressing FoxO3a significantly inhibited, whereas silencing endogenous FoxO3a enhanced, the growth and migration of HaCaT cells in vitro. Further investigation revealed that FoxO3a negatively regulated matrix metalloproteinases 1 and 9, and increased the expression of tissue inhibitor of metalloproteinase 1. In addition, the upregulation of FoxO3a retarded, whereas the downregulation of FoxO3a accelerated, transforming growth factorß1induced epithelialmesenchymal transition in HaCaT cells. Mechanistically, the overexpression of FoxO3a inactivated ßcatenin signaling and markedly reduced the levels of nuclear ßcatenin. These results reveal a novel mechanism of FoxO3a in regulating KC reepithelialization, and provide novel targets for the prevention and treatment of chronic wounds.