Treatment of Early Undifferentiated Chronic Monoarthritis of the Wrist by Arthroscopic Wrist Synovectomy Combined with Partial Denervation.

OBJECTIVE: Undifferentiated chronic monosecarthritis (UCMA) is a group of inflammatory joint diseases that has the potential to progress to other diseases and can seriously affect patients' quality of life. There is yet no unified consensus regarding treatment of UCMA. This study aimed to investigate the efficacy of arthroscopic synovectomy combined with partial wrist denervation in treating Larsen 1-3 UCMA. METHODS: In this case series, we reviewed 14 patients with UCMA treated by arthroscopic synovectomy combined with partial denervation from February 2017 to June 2020. The mean duration of symptoms was 17.4 months (range, 4-60 months), and the mean follow-up was 13.3 months (range, 6-23 months). The anterior and posterior interosseous nerves were severed at the distal forearm, and the radiocarpal, midcarpal, and distal radial ulnar joint synovial membranes were arthroscopically resected at the wrist. The clinical evaluation indices included the visual analogue scale score (VAS) for pain, grip strength, range of (active) motion of the wrist, total active motion, and Mayo wrist score. Larsen's scoring method was used as the imaging evaluation index. RESULTS: At the last follow-up, significant clinical improvements were observed in the visual analogue scale (VAS) score for pain (6.0 (5.0-6.3) vs 1.0 (1.0-2.3), P = 0.001) and Mayo wrist score (42.1 ± 9.7 vs 61.8 ± 12.3, P < 0.0001). No significant changes were found in grip strength (15.9 ± 4.5 vs 16.6 ± 4.7, P = 0.230) or the flexion-extension arc (58.9 ± 39.0 vs 64.3 ± 36.5, P = 0.317), although the mean and median did show positive changes. Among the three patients who showed progress in imaging, there was no significant difference in their pain and functional scores compared to those who did not progress. One patient underwent total wrist fusion 17 months after the operation. CONCLUSION: Arthroscopic wrist synovectomy combined with partial wrist denervation can provide sustained pain relief and functional recovery for patients with Larsen 1-3 UCMA.

Two-Year Clinical Outcomes after Arthroscopic Re-Excision of Recurrent Dorsal Wrist Ganglion Cyst.

OBJECTIVES: Arthroscopic excision of dorsal wrist ganglion (DWG) cysts has recently become an alternative to open surgery, with the advantage of lower recurrence. However, in recurrent cases, whether re-excision using an arthroscopic approach would achieve favorable outcomes has not been determined. This study aimed to evaluate the clinical outcomes of function evaluation and recurrence rate after arthroscopic excision of recurrent DWG cysts. METHODS: A total of 11 consecutive patients with clinically diagnosed recurrent DWG cysts were retrospectively reviewed between November 2017 and March 2020. Extensive re-excision of the ganglion cyst and its surrounding pathological capsule was performed using an arthroscopic approach. Magnetic resonance imaging (MRI) was routinely performed before surgery to identify the location and limits of the cyst. All patients were followed up for a minimum of 2 years. A second recurrence was recorded if a mass reappeared at the same site with a positive transillumination test. Pain during activity was evaluated using the visual analog scale (VAS). The active range of motion (ROM) of the wrist was measured using a goniometer, and the hand grip strength was measured using a digital dynamometer. Comparative analysis between the pre- and postoperative indexes was performed using Student's t-test. RESULTS: After a mean follow-up period of 29.3 months (range, 24-34 months), no second recurrence of the cyst was recorded. The VAS score improved from 1.4 to 0.3 (t = 3.833, p = 0.003), and residual pain was reported by three patients (VAS score = 1 for each). Active wrist flexion increased from 73.6° to 78.2° (t = 2.887, p = 0.016). No significant changes were found in active wrist extension or hand grip strength (p > 0.05). No major complications occurred during the study. CONCLUSION: Arthroscopic excision of a recurrent DWG cyst yielded satisfactory results with no second recurrence, significant pain relief, and good wrist function at a minimum of 2-year follow-up. Clear identification of the location and limits of the ganglion based on preoperative MRI could be helpful to achieve complete excision and therefore prevent a second recurrence.

Miniplate and K-Wire Fixation for Type Vb Jersey Finger: A Case Series of 9 Patients.

PURPOSE: The aim of this study was to present the results of plate and K-wire fixation for type Vb jersey finger. METHODS: We used a miniplate system combined with a K-wire to treat 9 cases of acute type Vb jersey finger injury. The fracture healing time, functional outcomes, and complications were assessed. RESULTS: The follow-up time ranged from 13 to 44 months. All fractures healed within 7 weeks. The mean active range of motion was 70.6° (60° to 80°) for the distal interphalangeal joint and 105° (100° to 110°) for the proximal interphalangeal joint. All affected fingers were able to move actively with no pain, and there was no nail deformity. Two patients with the longest radiographic follow-up showed a congruent joint surface with no degenerative change at 44 months. CONCLUSIONS: Miniplate and K-wire fixation offers adequate fixation strength for the early initiation of active exercises so that a good functional result can be achieved. TYPE OF STUDY/LEVEL OF EVIDENCE: Therapeutic V.

Centralization of ulna with wrist fusion for failure of reconstructed distal radius by allograft bone or prosthesis: A case report and literature review.

RATIONALE: Centralization of the ulna is commonly used in the treatment of radius developmental deformity in children. The secondary distal radius deficiency in adults is different from the developmental deformity of the radius in children. There is no report on the ulna centralization with wrist fusion for the failure of the reconstructed distal radius by allograft bone or prosthesis for osteosarcoma in adults. PATIENT CONCERNS: 2 patients with a bone tumor on the distal radius underwent tumor resection and distal radius reconstruction by allograft bone or prosthesis and suffered distal radius collapsed fracture and radiocarpal joint dislocation accompanied with moderate pain, severe deformity, and poor grip and pinching power several months follow primary surgery. DIAGNOSES: X-ray images revealed collapsed fracture of distal radius and dislocation of the radiocarpal joint. INTERVENTIONS: The 2 patients were operated on by the same technique under brachial plexus anesthesia. The allograft bone or prosthesis and the lunate were removed. The capitate was trimmed with a groove, and the cartilage surface with the subchondral bone of the distal ulna was resected to match the groove of the capitate. A straight plate with screws was used for internal fixation. OUTCOMES: Bone healing was achieved at 3 to 4 months after the surgery. After a minimum of 1-year follow-up, pain symptom was relieved, palmar flexion deformity was corrected, and grip and pinch strength were restored. The 2 patients were both satisfied with the improvement in appearance and function. LESSONS: For adult patients who have failed resection and reconstruction of giant cell tumors, osteosarcoma, and other tumors of bone on the distal radius, ulna centralization is a simple and effective option.

Evaluation of peri-prosthetic radiolucent lines surrounding the cementless femoral stem using digital tomosynthesis with metal artifact reduction: a cadaveric study in comparison with radiography and computed tomography.

BACKGROUND: The traditional criterion for the diagnosis of implant loosening in total hip arthroplasty (THA) was once defined as a radiolucent line of >2 mm in width, based on plain radiography. Recent progress in imaging technology has allowed for the identification of complete radiolucent lines of ≤2 mm around the whole prosthesis as the basis for diagnosing component loosening in the absence of component migration. This study aimed to compare the sensitivity and specificity of digital tomosynthesis with metal artifact reduction with those of radiography and conventional computed tomography (CT) for detecting radiolucent lines of ≤2 mm surrounding cementless femoral stems of different widths. METHODS: The medullary canals of 4 cadaveric femurs were broached to 13 mm in diameter. Cylindrical cementless femoral stems with diameters of 9, 10, 11, 12, and 13 mm were sequentially inserted into each femur, creating 5 groups of radiolucent lines 2.0, 1.5-1.6, 1.1-1.2, 0.5-0.6, and 0 mm in diameter, respectively. Imaging by tomosynthesis, radiography, and CT was conducted for each radiolucent line model. The width information of the radiolucent line models was used as a reference standard for calculating sensitivity and specificity: observations in the group of 0 mm were used for calculating specificity, and those in the other four groups were used for sensitivity. The differences in sensitivity and specificity between the imaging methods were compared with chi-square test, and the 95% confidence intervals of improvements in the sensitivity and specificity of tomosynthesis compared with radiography and CT were calculated using mixed effects models. RESULTS: The overall sensitivity of tomosynthesis (63.3%) for detecting radiolucent lines ≤2 mm wide was significantly higher (P<0.017) than that of radiography (20.5%) and CT (50.2%), an improvement of 58.2%±3.1% (95% CI, P<0.001) and 21.7%±7.1% (95% CI, P<0.001) compared to radiography and CT, respectively. The sensitivity values for detecting radiolucent lines in all four groups by tomosynthesis and CT were significantly higher than those of radiography (P<0.017). Tomosynthesis also had significantly higher sensitivity than CT (P<0.017) in detecting radiolucent line ≤1.2 mm wide. The specificity of TMAR, radiography, and CT for detecting radiolucent lines was 87.5%, 92.5%, and 82.5%, respectively, with no significant difference (P>0.017). CONCLUSIONS: Digital tomosynthesis with metal artifact reduction had significantly higher sensitivity than radiography for detecting radiolucent lines ≤2 mm wide surrounding cementless femoral stems. It also displayed higher sensitivity than CT for detecting radiolucent lines ≤1.2 mm in width. With a higher rate of detection for radiolucent lines narrower than 2 mm, tomosynthesis has the potential to improve the accuracy of early diagnosis of cementless THA stem loosening in clinical practice.

High-Intensity Ultrasound Treatment on Soy Protein after Selectively Proteolyzing Glycinin Component: Physical, Structural, and Aggregation Properties.

In this study, a novel method called selective proteolysis was applied to the glycinin component of soy protein isolate (SPI), and a degraded glycinin hydrolysate (DGH) was obtained. The effects of high-intensity ultrasound (HIU) treatment (20 kHz at 400 W, 0, 5, 20, and 40 min) on the physical, structural, and aggregation properties of DGH were investigated with the aim to reveal the influence of the selectively hydrolyzing glycinin component on the HIU treatment of soy protein. The effects of HIU on DGH and a control SPI (CSPI) were both time-dependent. HIU induced the formation of soluble aggregates in both samples at an early stage, while it dissociated these newly formed aggregates after a longer duration. Selectively hydrolyzing glycinin contributed to the soluble aggregation by exposing the compact protein structure and producing small protein fractions. The larger extent of hydrophobic interactions and disulfide bonds imparted a higher stability to the soluble protein aggregates formed in DGH. As a result, DGH displayed more ordered secondary structures, a higher solubility, and better gelling properties after the HIU treatment, especially at 20 min. The results of this study will be beneficial to the scientific community as well as industrial application.

Different femoral origins of valgus deformity affect aspect ratios of resected distal femurs in total knee arthroplasty.

PURPOSE: This study aimed to evaluate the anthropometry of resected distal femurs in valgus knees at the level of standard cuts during total knee arthroplasty (TKA), and to compare these measurements to neutrally aligned knees. METHODS: Anteroposterior and mediolateral measurements of the distal femur were performed on three-dimensional computed tomography reconstructions of 57 valgus knees (34 intra-articular valgus and 23 juxta-articular valgus) and 40 neutrally aligned knees. The measured dimensions and calculated aspect ratios (ARs) were subsequently compared. RESULTS: Juxta-articular valgus knees had similar ARs when compared with neutrally aligned knees (1.14 ±â€¯0.06 vs. 1.12 ±â€¯0.05, P = 0.103). However, intra-articular valgus knees had smaller ARs (1.09 ±â€¯0.07) when compared with juxta-articular valgus (P = 0.002) or neutrally aligned knees (P = 0.023). CONCLUSION: Different origins of valgus deformity in the femur can significantly affect the AR values on the resected surface of the distal femur. Pre-operative evaluation of a valgus deformity may assist in estimating the morphology of the resected distal femur during TKA.

Amino acid composition and nutritional value evaluation of Chinese chestnut (Castanea mollissima Blume) and its protein subunit.

The amino acid composition, nutritional value and proteins subunit of chestnuts (Castanea mollissima Blume) from three regions of China (Henan, Hunan, and Guangdong) were investigated. Experimental results showed that the albumin fraction dominated the chestnut protein composition, but globulin and prolamin were nondetectable. All the Chinese chestnut proteins had a nutritionally balanced amino acid composition. Their amino acid score (AAS) could reach the FAO/WHO (2013) requirement for essential amino acids for older children, adolescents and adults. Leucine was the first limiting amino acid for all the Chinese chestnut protein isolates and digestible indispensable amino acid scores (DIAAS) were more than 90 (Leu). The sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) spectra showed that different Chinese chestnut protein isolates had almost the same band components, which were mainly comprised of seven polypeptide segments with the molecular weights of (91-93) kDa, (70-72) kDa, (53-55) kDa, 37 kDa, (27-33) kDa, 20 kDa and (5-15) kDa. The amino acid compositions and the abundance of low molecular weight proteins indicated that Chinese chestnut could be utilized as a good source of plant protein for human nutrition.

Formation of amyloid fibrils from soy protein hydrolysate: Effects of selective proteolysis on ß-conglycinin.

The soy protein hydrolysate subjected to selective proteolysis on ß-conglycinin (referred to as DßH, contrast group) and a control soy protein isolate sample without addition of protease (referred to as CSPI, blank group) were adopted as experimental samples. By employing the "subtraction" mode of logical thinking, we aimed to compare the differences between CSPI and DßH on fibrillation at pH2.0 with heating at 95°C. The results showed when heated for 60min, CSPI tended to form short worm-like fibrils while DßH long semiflexible fibrils. When heating time was prolonged to 360min, the fibrils formed from them both exhibited cluster. Whereas when heated for 720min, no fibrillar aggregates appeared from them. This study would help explore the effects of ß-conglycinin on the fibril formation of soy protein isolate by a new way.

Carotenoid profiling of red navel orange "Cara Cara" harvested from five regions in China.

Understanding the composition and content of carotenoids in red-flesh navel orange "Cara Cara" cultivated in different regions is valuable for its nutrition assessment and fruit processing control. Herein, the carotenoids of "Cara Cara" fruits, harvested from five typical citrus growing regions of China, were identified and quantified by HPLC-DAD coupled with HPLC-DAD-APCI-MS/MS. In saponified extracts, 19 free carotenoids including 8 carotenes and 11 xanthophylls were identified. In unsaponified extracts, 83.83-93.83% xanthophylls were esterified and 19 esters were inferred, with the 9-cis-violaxanthin ester confirmed as the dominant component. The carotenoid contents showed difference among the "Cara Cara" fruits from different growing regions, which might be attributed to the local environmental conditions, and the temperature was found directly correlated with total carotenoids content of "Cara Cara". The data obtained in this study will facilitate the nutritional evaluation of "Cara Cara" and provide beneficial guidance for fruit processing.

Screw-hole clusters in acetabular cups: a morphological study of optimal positioning of screw-holes.

BACKGROUND: Rigid and safe transacetabular screw fixation in total hip arthroplasty (THA) is achieved by pursuing deeper bone stock and avoiding injuries to the neurovascular structures, but these efforts can be restricted by the distribution of screw-holes on cups by the manufacturer. We therefore tried to determine: (i) optimal screw-hole positions on cups to allow anatomical placement of screws; (ii) rationality of the basic 3-screw-hole cluster on commercial cups; and (iii) the optimum method for placing commercial cups in accordance with acetabular anatomy. METHODS: Periacetabular osseous structure of 64 hips and arterial structures of 50 hips were three-dimensionally reconstructed. Simulated transacetabular screw fixation during THA was performed in these hips with 3 different screw lengths (15, 25, and 35 mm) to define deeper and safer screw trajectories. Screw-hole locations of 7 commercially available cups were measured and matched with the periacetabular anatomy. RESULTS: When the cup was placed into the acetabulum at 45° of abduction and 20° of anteversion, the optimal locations of 2 screw-holes on the cups were at 30° and 64° of latitude, with a 35° separation angle. The inversetriangle distribution pattern was safer than the triangle pattern in basic 3-screw-hole-cluster cups. When placing the commercial cups, 5°-10° of anterior rotation can be added to allow better screw trajectories. CONCLUSIONS: Our study determined optimal screw-hole positions and their distribution pattern on cups. We describe methods to place the commercial cups that are not designed according to acetabular anatomy.

Effect of ultrasonic processing on the changes in activity, aggregation and the secondary and tertiary structure of polyphenol oxidase in oriental sweet melon (Cucumis melo var. makuwa Makino).

BACKGROUND: Polyphenol oxidase (PPO) mainly contributes to the browning reaction of fruits and vegetables and causes serious damage to the quality of sweet melon products. However, traditional methods to inactivate browning may induce more unexpected risks than ultrasonic processing. Meanwhile, there are no reports on the effect of ultrasound on PPO directly purified from sweet melon. RESULTS: The PPO in the original juice was less inactivated than the purified form when treated with ultrasound. As for purified PPO, superior to thermal treatment, less heat was needed to inactivate the PPO with ultrasonic treatment. At intensity lower than 200 W, ultrasound did not significantly affect the structure and activity of PPO (P > 0.05), and latent PPO was activated. At intensity higher than 200 W, ultrasound inactivated PPO, induced aggregation and dissociation of PPO particles and significantly decreased the α-helix structure content. CONCLUSION: Low-frequency high-intensity ultrasound caused an inactivation effect and conformational changes of purified PPO from oriental sweet melons. Changes in the PPO structure induced by ultrasound eventually inactivated the enzyme. Ultrasound may be a potential method to inactivate PPO in oriental sweet melons. © 2016 Society of Chemical Industry.

Yield improvement of exopolysaccharides by screening of the Lactobacillus acidophilus ATCC and optimization of the fermentation and extraction conditions.

Exopolysacharides (EPS) produced by Lactobacillus acidophilus play an important role in food processing with its well-recognized antioxidant activity. In this study, a L. acidophilus mutant strain with high-yielding EPS (2.92±0.05 g/L) was screened by chemical mutation (0.2 % diethyl sulfate). Plackett-Burman (PB) design and response surface methodology (RSM) were applied to optimize the EPS fermentation parameters and central composite design (CCD) was used to optimize the EPS extraction parameters. A strain with high-yielding EPS was screened. It was revealed that three parameters (Tween 80, dipotassium hydrogen phosphate and trisodium citrate) had significant influence (P < 0.05) on the EPS yield. The optimal culture conditions for EPS production were: Tween 80 0.6 mL, dipotassium hydrogen phosphate 3.6 g and trisodium citrate 4.1 g (with culture volume of 1 L). In these conditions, the maximum EPS yield was 3.96±0.08 g/L. The optimal extraction conditions analyzed by CCD were: alcohol concentration 70 %, the ratio of material to liquid (M/L ratio) 1:3.6 and the extraction time 31 h. In these conditions, the maximum EPS extraction yield was 1.48±0.23 g/L. It was confirmed by the verification experiments that the EPS yield from L. acidophilus mutant strains reached 5.12±0.73 g/L under the optimized fermentation and extraction conditions, which was 3.8 times higher than that of the control (1.05±0.06 g/L). The results indicated that the strain screening with high-yielding EPS was successful and the optimized fermentation and extraction conditions significantly enhanced EPS yield. It was efficient and industrially promising.

Effect of high intensity ultrasound on transglutaminase-catalyzed soy protein isolate cold set gel.

The effects of high intensity ultrasound (HIU, 105-110 W/cm(2) for 5 or 40 min) pre-treatment of soy protein isolate (SPI) on the physicochemical properties of ensuing transglutaminase-catalyzed soy protein isolate cold set gel (TSCG) were investigated in this study. The gel strength of TSCG increased remarkably from 34.5 to 207.1 g for TSCG produced from SPI with 40 min HIU pre-treatment. Moreover, gel yield and water holding capacity also increased after HIU pre-treatments. Scanning electron microscopy showed that HIU of SPI resulted in a more uniform and denser microstructure of TSCG. The content of free sulfhydryl (SH) groups was higher in HIU TSCG than non-HIU TSG, even though greater decrease of the SH groups present in HIU treated SPI was observed when the TSCG was formed, suggesting the involvement of disulfide bonds in gel formation. Protein solubility of TSCG in both denaturing and non-denaturing solvents was higher after HIU pretreatment, and changes in hydrophobic amino acid residues as well as in polypeptide backbone conformation and secondary structure of TSCG were demonstrated by Raman spectroscopy. These results suggest that increased inter-molecular ε-(γ-glutamyl) lysine isopeptide bonds, disulfide bonds and hydrophobic interactions might have contributed to the HIU TSCG gel network. In conclusion, HIU changed physicochemical and structural properties of SPI, producing better substrates for TGase. The resulting TSCG network structure was formed with greater involvement of covalent and non-covalent interactions between SPI molecules and aggregates than in the TSCG from non-HIU SPI.

Detection of bacterial concentration variations based on dielectric magnetic flux.

This study is based on the development of bacterial count quantification generated by medium dielectric variations and consequent polarization material release. The proposed approach is an action method for the fast detection of bacterial concentration in orange juice. The sensing method relies on bacterial attachment up to biofilm formation. Furthermore, different media provide more oxygen group content to enhance capacitance and self-discharge. The test took only 30 min and it provided the means for rapid bacterial detection in the juice industry.

Antimicrobial application of nanofibrous mats self-assembled with quaternized chitosan and soy protein isolate.

Positively charged N-[(2-hydroxy-3-trimethylammonium) propyl] chitosan chloride (HTCC) and negatively charged soy protein isolate (SPI) were alternately assembled on cellulose acetate (CA) electrospun nanofibrous mats via electrostatic layer-by-layer self-assembly technique. CA nanofibrous mats coated with bilayers of HTCC and SPI possessed more orderly-arranged structure than uncoated CA mats according to the observation from scanning electron microscopy images. The average diameter of the nanofibers was enlarged by the increase of the bilayer number. X-ray photoelectron spectroscopy indicated that HTCC and SPI were coated on the surface of the CA mats successfully. The average diameters of inhibition zones of (HTCC/SPI)10.5-films-coated nanofibrous mats against Escherichia coli and Staphylococcus aureus were 9.6mm and 11.53mm, respectively, which demonstrated the highest antimicrobial activity among samples in presented study.

Characterization of germin-like protein with polyphenol oxidase activity from Satsuma mandarine.

Polyphenol oxidases (PPOs) catalyzing the oxygen dependent oxidation of phenols to quinones are ubiquitously distributed in plants and are assumed to be involved in plant defense against pests and pathogens. A protein with high PPO activity was identified in Satsuma mandarine, extracted with Tris-HCl buffer, purified by salt precipitation and column chromatography, and characterized by mass spectrometry as germin-like protein (GLP), which belongs to pathogenesis related protein (PR) family. In the present study, the structure and enzymatic properties of GLP were characterized using spectroscopy methods. Based on native PAGE analysis, the molecular weight of GLP was estimated to be 108 kDa and GLP was identified as a pentamer containing five subunits of 22 kDa. The optimum pH and temperature for PPO catalyzing activity of GLP was 6.5 and 65°C, respectively. Kinetic constants were 0.0365 M and 0.0196 M with the substrates catechol and pyrogallol, respectively. The structural characterization of GLP provided better insights into the regions responsible for its PPO activity.

Physicochemical properties of peanut protein isolate-glucomannan conjugates prepared by ultrasonic treatment.

Peanut protein isolate (PPI) was glycated with glucomannan through classical heating or ultrasound treatment in this work. The physicochemical properties of PPI-glucomannan conjugates prepared by ultrasound treatment were compared to those prepared by classical heating. Compared with classical heating, ultrasound treatment could accelerate the graft reaction between PPI and glucomannan and improve the concentration of available free amino groups of PPI. Solubility and emulsifying properties of the conjugates obtained by ultrasound treatment were both improved as compared to those obtained by classical heating and native PPI. Decreases of lysine and arginine contents during the graft reaction indicated that these two amino acid residues attended the covalent linkage between PPI and glucomannan. Structural feature analyses suggested that conjugates obtained by ultrasound treatment had less α-helix, more ß-structures and random coil, higher surface hydrophobicity and less compact tertiary structure as compared to those obtained by classical heating and native PPI.

Physicochemical and functional properties of micronized jincheng orange by-products (Citrus sinensis Osbeck) dietary fiber and its application as a fat replacer in yogurt.

Orange by-products from juice extraction are generally discarded or used in animal feed due to their low market value. However, orange by-products show potential as dietary fiber (DF) and fat replacers in products such as yogurt. This study assessed the benefits of using orange by-products in DF-enriched materials such as DF powders (OP) and micronized DF with ball-milling (MDF). The study also investigated the effects of adding different levels of OP and MDF on the quality of low-fat yogurt. Results show that MDF showed better physicochemical and functional properties than OP, and that 2% MDF as a fat replacer in yogurt retained most of the textural and sensory properties of full-fat yogurt. Therefore, this study showed that MDF is a promising alternative as a fat replacer in low-fat yogurt, without sacrificing good taste and other qualities of full-fat yogurt.

Acid-induced gelation behavior of soybean protein isolate with high intensity ultrasonic pre-treatments.

High intensity ultrasonic (HUS, 20 kHz, 400 W) pre-treatments of soybean protein isolate (SPI) improved the water holding capacity (WHC), gel strength and gel firmness (final elastic moduli) of glucono-δ-lactone induced SPI gels (GISG). Sonication time (0, 5, 20, and 40 min) had a significant effect on the above three properties. 20 min HUS-GISG had the highest WHC (95.53 ± 0.25%), gel strength (60.90 ± 2.87 g) and gel firmness (96340Pa), compared with other samples. Moreover, SH groups and non-covalent interactions of GISG also changed after HUS pre-treatments. The HUS GISG had denser and more uniform microstructures than the untreated GISG. Rheological investments showed that the cooling step (reduce the temperature from 95 to 25 °C at a speed of 2 °C/min) was more important for the HUS GISG network formation while the heat preservation step (keep temperature at 95 for 20 min) was more important for the untreated GISG. HUS reduced the particle size of SPI and Pearson correlation test showed that the particle size of SPI dispersions was negatively correlated with WHC, gel strength and gel firmness.