Solid substrate-room temperature phosphorimetry for the determination of trace terbutaline sulfate based on its inhibition oxidation of rhodamine 6G by sodium periodate.

When 1.00 mol l(-1) I(-) is used as ion perturber, rhodamine 6G (Rh 6G) can emit strong and stable room temperature phosphorescence (RTP) on filter paper substrate in KHC(8)H(4)O(4)-HCl buffer solution (pH = 3.50), heated at 70 degrees C for 10 min. NaIO(4) can oxidize Rh 6G, which makes the RTP signal quench. Terbutaline sulfate (TBS) can inhibit NaIO(4) from oxidizing Rh 6G, which makes the RTP signal of Rh 6G enhance sharply. The content of TBS is linear correlation to DeltaIp of the system. Based on the facts above, a new inhibition solid substrate-room temperature phosphorimetry (SS-RTP) for the determination of trace TBS has been established. The linear range of this method is 0.0104-2.08 pg spot(-1) (corresponding concentration: 0.026-5.2 ng ml(-1), with a sample volume of 0.4 microl) with a detection limit (L.D.) of 2.6 fg spot(-1) (corresponding concentration: 6.5 x 10(-12) g ml(-1)), and the regression equation of working curve is DeltaIp = 2.040 + 54.54 m(TBS) (pg spot(-1)), n = 6, correlation coefficient is 0.9994. For the samples containing 0.0104 pg spot(-1) and 2.08 pg spot(-1) TBS, the relative standard deviation (RSD) are 3.8% and 2.3% (n = 8), respectively, indicating good precision. This method has been applied to determination of trace TBS in the practical samples with satisfactory results. The reaction mechanism of NaIO(4) oxidizing Rh 6G to inhibit SS-RTP for the determination of trace TBS is also discussed.

Solid substrate-room temperature phosphorescence method for the determination of trace Mn(II) based on oxidizing reaction of hydrogen peroxide using alpha,alpha'-bipyridine as sensitizer.

A new solid substrate-room temperature phosphorescence (SS-RTP) method for the determination of trace manganese (II) has been established. It bases on the fact that fullerol (R) emits strong and stable room temperature phosphorescence (RTP) on filter paper substrate. H2O2 can oxidize R to cause the SS-RTP quenching. But manganese (II) can obstruct H2O2 to oxidize R, and enhance the RTP of R. alpha,alpha'-Bipyridine (Bipy) can sensitize the RTP. After adding Bipy, the DeltaI(p) enhances 7 times than that without Bipy. Under the optimum conditions, the linear dynamic range of this method is 0.016-1.12 pg spot(-1) with a detection limit (L.D.) of 4.6 fg spot(-1) (m(Mn(2+) is the absolute mass of Mn(2+)), and the regression equation of working curve is DeltaI(p)=25.20 + 63.55 m(Mn(2+) (pg spot(-1)), n=6, r=0.9983. For 0.016 and 1.12 pg spot(-1) Mn(2+), RSDS are 4.3 and 4.8%, respectively (n=7). This method has been applied to the determination of trace manganese (II) in actual sample with high sensitivity and good selection. And the reaction mechanism of SS-RTP is discussed.