RESUMO
:To investigate the effect of transient receptor potential melastatin 2 ï¼TRPM2ï¼ inhibitor A10 on oxygen glucose deprivation/reperfusion ï¼OGD/Rï¼ injury in SH-SY5Y cellsï¼:Human neuroblastoma SH-SY5Y cells were subject to OGD/R injuryï¼and then were divided into blank control groupï¼model control group and A10 group randomly. The cell survival rate was detected by cell counting kit 8 ï¼CCK-8ï¼; the level of cellular reactive oxygen species ï¼ROSï¼ was detected by reactive oxygen detection kit; the mitochondrial membrane potential was detected by tetramethylrhodamine ï¼TMRMï¼ method; the number of apoptotic cells was detected by TUNEL apoptosis assay kit; the protein expression level of cleaved caspase 3 was detected by Western blotï¼:Compared with 3ï¼20ï¼30ï¼50ï¼ has lower cytotoxicity and better inhibition effect on channel activity. Compared with the model control groupï¼ROS level was reducedï¼the mitochondrial membrane potential was improvedï¼the number of apoptosis cells was reduced ï¼and the expression of cleaved caspase 3 was significantly reduced in the A10 groupï¼all <0.05ï¼. : A10 can alleviate cell damage after OGD/R by inhibiting TRPM2 channel functionï¼reducing extracellular calcium influxï¼reducing cell ROS levelsï¼stabilizing mitochondrial membrane potential levelsï¼and reducing apoptosis.
Assuntos
Glucose , Canais de Cátion TRPM , Apoptose , Benzenoacetamidas , Sobrevivência Celular , Humanos , Oxigênio/metabolismo , Piperidonas , Espécies Reativas de Oxigênio/metabolismo , ReperfusãoAssuntos
Doença de Graves , Metimazol , Antitireóideos , Humanos , Tomografia , Tomografia Computadorizada de EmissãoAssuntos
Neoplasias da Mama , Metástase Linfática , Humanos , Linfonodos , Biópsia de Linfonodo SentinelaRESUMO
OBJECTIVE: To observe the effect of Puerarin Injection on the hemorheology in acute blood-stasis model rats. METHOD: The acute blood-stasis model rats were made by being soaked in ice water afer being injected adrenaline hydrochloride injection in a major dose. The changes of viscosity of whole blood and plasma, blood yield stress, erythrocyte aggregation and the maximum rate of platelet aggregation in the acute blood-stasis model rats were measured with Auto-Viscometer, and then the influence of Puerarin Injection on the hemorheology in the model rats was investigated. RESULT: The viscosity of whole blood and plasma, and blood yield stress in the acute blood-stasis model rats were significantly higher than those in the normal control group (P < 0.01, P < 0.05). Both the high dose and the low dose of Puerarin Injection could reduce the viscosity of whole blood and plasma, blood yield stress and the maximum rate of platelet aggregation in the acute blood-stasis model rats (P < 0.01, P < 0.05). The high dose could also reduce the erythrocyte aggregation and the deformed Index of red blood cell (P < 0.05). CONCLUSION: Puerarin Injection can ameliorate the hemorheology in acute blood-stasis model rats, and it has a dose-response relationship.
