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ObjectiveTo discuss the impact of Buzhong Yiqitang on lipid metabolism in skeletal muscle of exercise-induced fatigue （EIF） mice through adiponectin receptor 1 （Adipor1）/adenosine 5'-monophosphate（AMP）-activated protein kinase （AMPK）/peroxisome proliferator-activated receptor gamma coactivator-1α （PGC-1α）. MethodC57BL6J mice were randomly divided into the control group， model group， low， middle， and high dose groups of Buzhong Yiqitang， and vitamin C group. No intervention was given to the control group， while the other groups were subjected to exhaustive swimming training to establish the EIF model. One hour before exhaustion， 0.2 mL distilled water was given to the control group and the model group， while the mice in the low， middle， and high dose groups of Buzhong Yiqitang were given intragastrically Buzhong Yiqitang of 4.1， 8.2， and 16.4 g·kg-1， respectively， and the vitamin C group was given vitamin C of 0.04 g·kg-1 via gavage for a duration of six weeks. After six weeks of the experiment， the growth rate of body weight， organ index， and exhaustive swimming time were calculated. Enzyme colorimetry was utilized to detect the levels of blood urea nitrogen （BUN）， creatine kinase acid （CK）， lactate dehydrogenase acid （LDH）， and lactic acid （LD）. The pathological changes of skeletal muscle were observed using hematoxylin -eosin （HE） staining， while the ultrastructure of skeletal muscle was observed with transmission electron microscope （TEM）. The contents of free fatty acids （NEFA） and triglyceride acid （TG） in serum were also examined by microplate method. The protein expressions of Adipor1， p-AMPK/AMPK， PGC-1α， and HK2 in the skeletal muscle were measured by Western blot. ResultCompared with those of the control group， the growth rate of body weight and thymus index of the model group were decreased， and the serum levels of BUN， CK， LD， and LDH were increased （P<0.01）. The contents of NEFA and TG were decreased （P<0.01）， and the protein expression of Adipor1， p-AMPK/AMPK， PGC-1 α， and HK2 in the skeletal muscle decreased （P<0.05， P<0.01）. Compared with those in the model group， the growth rate of body weight， thymus index， and exhaustive swimming time were significantly increased （P<0.01）， and the levels of BUN， CK， LD， and LDH dropped in the high dose group of Buzhong Yiqitang （P<0.01）. The levels of NEFA and TG were greatly improved （P<0.01）. The protein expressions of Adipor1， p-AMPK/AMPK， PGC-1α， and HK2 in the skeletal muscle were significantly increased （P<0.05， P<0.01）. Compared with those in the model group， the thymus index and exhaustive swimming time were significantly increased in the vitamin C group， and the levels of BUN， CK， and LD dropped （P<0.05， P<0.01）. The levels of NEFA and TG were improved significantly （P<0.01）， and the protein expression of Adipor1 in skeletal muscle was increased greatly （P<0.01）. ConclusionBuzhong Yiqitang can delay the development of EIF， which may be connected with the regulation of the Adipor1/AMPK/PGC-1α signaling pathway and the improvement of the utilization rate of skeletal muscle to fat.
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At present, four operating modes have been explored for physical-medical integration in China: hospital health guidance center, sports club health guidance, community health monitoring center and industry-university-research cooperation on the integration of physical and medical services. However, physical-medical integration is still in its infancy, the cultivation of physical-medical integration talents has been a key to the deep development of "physical-medical integration". The researches show that the current training mode of integrative talents mainly focuses on on-the-job training, which is faced with many problems such as serious shortage of specialized talents, imperfect certification system and single talent training mode. It is suggested that the training system, the curriculum system, the public opinion system and the professional standards should be improved so as to realize the scientific and sustainable development of physical-medical talents.
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Objective To study the influence of swimming training for different periods on rat liver function, and to evaluate the quality of exercise-induced fatigue rat model established by swimming training for different periods. Methods Forty SD rats were randomly divided into 5 groups, namely blank group, and 7-, 14-, 21-and 28-day exercise groups, 8 rats in each group. Rats in the blank group were fed with normal diet, but were not given exercise training. The other groups were given exercise training for 7, 14, 21 and 28 days respectively, two times a day, 15 minutes every time and with 10-minute resting interval between the two times. At the last day of the experiment, all rats were executed by neck dislocation for the sampling of blood and liver tissues, and then the serum contents of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined. And the contents of total anti-oxidization capacity (T-AOC), malondialdehyde (MDA), lactic acid (LA), lactate dehydrogenase (LDH) and glucose (Glu) in liver tissues were also examined. Results Compared with the blank group, the contents of ALT and AST were increased in 7-, 14-, 21-, and 28-day exercise groups, especially in 21-and 28-day exercise groups (P<0.01) . The contents of T-AOC and MDA were increased in 7-, 14-, 21-, and 28-day exercise groups (P<0.01), the effects on T-AOC in the 21-and 28-day exercise groups and on MDA in the 28-day exercise group being more obvious (P<0.05 or P<0.01) . The LA content and LDH activity were increased and the glycogen was decreased in the liver tissues of 7-, 14-, 21-, and 28-day exercise groups, and the increase of LA content in 21- and 28-day exercise groups and that of LDH activity in 28-day exercise groups were more significant ( P<0.05 or P<0.01). The hepatic glycogen content in 14-, 21-, and 28-day exercise groups were lower than that in the other groups (P<0.01). Conclusion With hepatic function injury in exercise-induced fatigue as the criteria, the exercise-induced fatigue rat model has been successfully established in this study, and the model established by swimming training for 21 and 28 days is more steady.
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Objective To investigate into social cognition of new subject physical education in Chinese Medicine colleges and to find out existing issues and institutes strategy to solve them. Methods Questionnaire and analysis of documents and telephonic interview were adopted. Results Society needs a large number of graduates who have solid knowledge of health protection and a good command of methods of preseving health and keeping fit and at the same time requires them to go in for teaching athletics, training and organizing games. New subject Physical Education has some advantages,and there are also lots of difficulties. Conclusions Course setting should be sptimized, practical teaching should be strengthened and publicized to ensure that the cultivation of talents meets the need of society.
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Objective To observe the effects of ginsenoside Rg1 group on the structure and function of rat skeletal muscle with exercise-induced fatigue.Methods SD rats were randomized into blank control group,model group and ginsenoside Rg1 group.The rats in the model group and ginsenoside Rg1 group received medium-intensity treadmill running to induce exercise-induced fatigue.Ginsenoside Rg1 group was given ginsenoside Rg1 50 mg?kg-1?d-1 for 2 continuous weeks during the modeling.After treatment,malondialdehyde(MDA)content,superoxide dismustase(SOD) activity,mitochondrial membrane potential and free calcium content in the rat skeletal muscle were measured.Meanwhile,ultrastructure of the skeletal muscle was observed under the electron microscope.Results In the model group,SOD activity,mitochondrial membrane potential,and free calcium content were decreased,MDA content was increased,and the difference was significant as compared with the blank control group(P
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Objective To observe the effect of Baduanjin,a health Qigong,on the rehabilitation of type 2 diabetes mellitus(T2DB)patients.Methods Forty-eight T2DB patients were equally randomized into two groups:group A received routine medicine and Baduanjin exercises,and group B received medicine only,the treatment lasting 24 weeks.The vital capacity(VC),body mass index(BMI),waist-hip ratio,blood glucose and blood lipid levels were detected before and after treatment.Results Compared with those before treatment, the number of patients with increased VC under conditions of standing still or being loaded was increased obviously, BMI and waist-hip ratio were decreased,total cholesterol(TC),fasting blood glucose(FBG)and glycosylated hemoglobin(GHB)levels were decreased,and high-density lipoprotein cholesterol(HDL-C)level was increased in group A after doing Baduanjin exercises(P
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Objective This report describes the mechanism of Ginsenoside Re to resist Exercise-induced fatigue based on the observation of effects on MDA content and SOD activity.Methods Thirty male SD rats were divided into 3 groups randomly:Ginsenoside Re group,model group and control group,10 rats in each group.Rats in Ginsenoside Re group were given gastric gavage of Ginsenoside Re once a day,while rats in model and control groups were given the same volume of normal saline.One hour after administration,rats of Ginsenoside Re groups and Model groups received medium-intensity treadmill exercise for 20 minutes at the speed of 15m/min,with a slope of 0 degree,and after 40-mimute break repeated it for another 20minutes.Fourteen days later,MDA content and SOD activity have been tested.Results MDA content in the serum,liver tissue and muscle tissue of Ginsenoside Re group was obviously lower than that in the model group (P