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Toxicon ; 133: 82-90, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-28478060

RESUMO

The cytokine storm induced by staphylococcal enterotoxin B (SEB) describes the rapid and dramatic induction of mediators which are likely responsible for the toxin's deleterious effects. However despite the use of numerous animal models for investigating SEB related illness in humans, mechanisms of toxicity and correlates of protection remain unclear. In the present study, we used an LPS-potentiated model of SEB lethality to investigate the toxin-induced cytokine and chemokine responses in untreated and immunized mice. Of 30 separate mediators analyzed, serum levels for 28 or 27 of these cytokines and chemokines were elevated following administration of dosages of 3 or 30 LD50 of native SEB, respectively. Mice immunized with a non-toxic SEB vaccine candidate expressed in either E. coli or transgenic soy expression systems were protected from lethality when challenged with potentiated SEB. The majority of SEB-induced cytokines and chemokines (21 of 28 or 23 of 27 following challenge with dosages of 3 or 30 LD50 of native SEB, respectively) were significantly decreased in mice immunized with an SEB vaccine candidate when compared to control animals. Together, these studies provide the most comprehensive evaluation of the cytokine storm induced in this LPS-potentiated model of SEB lethality to date. As with other animal models, the identification of those mediators which are necessary and sufficient for SEB-induced toxicity remains unclear.


Assuntos
Quimiocinas/sangue , Citocinas/sangue , Enterotoxinas/imunologia , Enterotoxinas/toxicidade , Imunização , Vacinas Antiestafilocócicas/imunologia , Animais , Quimiocinas/imunologia , Quimiocinas/metabolismo , Citocinas/imunologia , Citocinas/metabolismo , Modelos Animais de Doenças , Escherichia coli/genética , Lipopolissacarídeos/administração & dosagem , Camundongos Endogâmicos BALB C , Plantas Geneticamente Modificadas/genética , Glycine max/genética , Vacinas Antiestafilocócicas/administração & dosagem , Vacinas Antiestafilocócicas/isolamento & purificação , Vacinas Sintéticas/administração & dosagem , Vacinas Sintéticas/imunologia , Vacinas Sintéticas/isolamento & purificação
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