RESUMO
BACKGROUND: Before the publication of the M40-A standard by the United States National Committee for Clinical Laboratory Standards (NCCLS), no quality control data for swab transport systems (STSs) had been available. AIMS: To compare three commercially available STSs against the published standard to ascertain bacterial survival after a set holding time. METHOD: Charcoal and non-charcoal containing swabs were inoculated with standard volumes and numbers of a range of bacteria commonly isolated from clinical material. Bacterial counts were taken at time zero, six, 24, and 48 hours, with the results being compared against the NCCLS standard. RESULTS: The standard clearly differentiated between the three STSs tested, with one product being superior to the other two products in providing better survival. CONCLUSION: The standard now provides manufacturers and users of STSs with meaningful data to provide, purchase, and compare an STS for routine clinical use.
Assuntos
Técnicas Bacteriológicas/normas , Manejo de Espécimes/normas , Meios de Transporte/normas , Bactérias/crescimento & desenvolvimento , Carvão Vegetal , Contagem de Colônia Microbiana , Meios de Cultura , Humanos , Controle de Qualidade , Manejo de Espécimes/métodos , Meios de Transporte/métodosRESUMO
Four tasks relying on short-term retention, i.e. digit span, missing scan, randomization span, and pointing span were presented to patients with frontal-lobe lesions and normal controls. Digit span was considered a measure of passive short-term memory, the other three tasks additionally reflect comparing processes, and two of these, the randomization and pointing spans, also measure active ordering by the subject. The results confirmed Petrides and Milner's (Neuropsychologia 20, 249-262, 1982) hypothesis that frontal-lobe patients are typically deficient in subject-ordered tasks. It is also suggested that they are deficient in a spontaneous generation, rather than comparison component of subjective ordering.
Assuntos
Neoplasias Encefálicas/cirurgia , Dominância Cerebral/fisiologia , Lobo Frontal/cirurgia , Memória de Curto Prazo/fisiologia , Complicações Pós-Operatórias/fisiopatologia , Desempenho Psicomotor/fisiologia , Psicocirurgia , Aprendizagem Seriada/fisiologia , Neoplasias Encefálicas/fisiopatologia , Feminino , Lobo Frontal/fisiopatologia , Humanos , Masculino , Rememoração Mental/fisiologiaRESUMO
Experience with 147 operations for extracranial cerebral arterial reconstruction in 122 patients over a 36-month period is presented. The commonest condition in white, black and indian patients was atherosclerosis, although 8 of the 23 black patients had nonspecific aorto-arteritis (Takayasu's disease). Operations within the mediastinum included bypass from the ascending aorta to major arterial branches (14), brachiocephalic endarterectomy (1), and replacement grafts of carotid (1) and subclavian (1) arteries. Cervical procedures included carotid endarterectomy or reconstruction (115), carotid-subclavian artery bypass (13) and axillary-to-axillary artery crossover (2). Both early and longer-term results are comparable with those reported in the surgical literature.
Assuntos
Isquemia Encefálica/cirurgia , Revascularização Cerebral , Adulto , Idoso , Prótese Vascular , Artérias Carótidas/cirurgia , Endarterectomia , Feminino , Humanos , Masculino , Mediastino/cirurgia , Pessoa de Meia-Idade , Pescoço/cirurgia , Artéria Subclávia/cirurgiaRESUMO
Operative treatment of nonspecific aortoarteritis remains controversial and little information is available on the results of reconstruction of extracranial cerebral vasculature in this disease. Our experience with 25 patients with histologically proven symptomatic disease treated during a 4-year period is presented. The aortic arch and its branches were involved in 12 patients and 13 had disease affecting the descending aorta and its tributaries. Patients with cerebrovascular disease had aneurysms, minor stroke, or intermittent neurologic dysfunction. Descending aortic involvement resulted either in symptomatic or ruptured aneurysm and renovascular hypertension. Operative treatment of cerebrovascular disease comprised aortic arch (three patients), carotid (three patients), or subclavian artery reconstruction (six patients). Descending aortic reconstruction comprised thoracoabdominal (four patients) or infrarenal (five patients) aneurysmorrhaphy, abdominal aortic replacement with bilateral renal artery reconstruction (two patients), and nephrectomy (two patients). One early postoperative death occurred because of stroke. Twenty-four survivors have been observed between 3 and 42 months. No deaths or further neurologic episodes have occurred during this period and three of five hypertensive patients were cured. We conclude that symptomatic aortoarteritis, including cerebrovascular disease, may be treated by standard operative techniques with rewarding results.
Assuntos
Síndromes do Arco Aórtico/cirurgia , Arterite de Takayasu/cirurgia , Adolescente , Adulto , Criança , Pré-Escolar , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Arterite de Takayasu/classificação , Arterite de Takayasu/patologiaRESUMO
A total of 137 strains of Gardnerella vaginalis were examined by the API 20 Strep system. The system was shown to be reliable when the tests were compared with standard identification methods, and very little confusion occurred with streptococcal profiles; consequently, G. vaginalis has been included in the API 20 Strep data base.
Assuntos
Gardnerella vaginalis/classificação , Haemophilus/classificação , Vaginite/microbiologia , Feminino , Gardnerella vaginalis/isolamento & purificação , Humanos , Kit de Reagentes para DiagnósticoRESUMO
Over a 36-month period 201 patients came to the Vascular Service, King Edward VIII Hospital, with atherosclerotic occlusive disease involving the femoropopliteal or femoro-infrapopliteal segments. On the basis of angiographic and clinical assessment 113 patients underwent bypass procedures and in 88 amputation was performed. The two groups were well matched as regards age and clinical condition. In-hospital mortality was 3,5% and 2,3% in the bypass and amputation groups respectively and the time spent in hospital was 5-6 weeks. In the longer term the desired achievement was limb salvage or ambulation on a prosthesis. Cumulative life-table analysis showed that more patients in the limb-salvage group than amputees were ambulant over any given time interval, although at 36 months after operation there was only an 8-10% difference. The results suggest that it is worth while attempting limb salvage in these cases, certainly as far as the first 3 years after operation are concerned.
Assuntos
Amputação Cirúrgica , Arteriosclerose/cirurgia , Artéria Femoral , Artéria Poplítea , Amputação Cirúrgica/reabilitação , Arteriosclerose/reabilitação , Prótese Vascular , Artéria Femoral/cirurgia , Humanos , Pessoa de Meia-Idade , Polietilenotereftalatos , Politetrafluoretileno , Artéria Poplítea/cirurgia , Complicações Pós-Operatórias , RiscoRESUMO
The study was undertaken to define and compare the incidence of bacterial contamination on opening three different types of hypodermic needle package. Four user groups were studied (anaesthetists, intensive care nurses, operating department assistants and medical laboratory scientific officers). Bacterial contamination was shown to occur and to be related to the type of needle packaging, the ratio of contamination incidence being similar in all four user groups. The results emphasize the importance of a careful opening technique when using sterile packaged hypodermic needles.
Assuntos
Anestesiologia/instrumentação , Agulhas , Bactérias/isolamento & purificação , Equipamentos Descartáveis , Humanos , EsterilizaçãoRESUMO
The management of 85 patients with penetrating injuries involving the external (n = 19), internal (n = 6) and common carotid (n = 46), vertebral (n = 10) and brachiocephalic (n = 4) arteries over a 5-year period in one hospital is reviewed. Sixty-three patients had no peroperative neurological deficit; 22 presented with localizing neurological signs. Where there was no deficit, the external carotid (n = 19) and vertebral (n = 10) arteries were ligated without adverse sequelae. Arterial reconstruction was performed of the internal carotid (n = 2) which resulted in a temporary neurological deficit in 7 patients and death in 3. Among the 22 patients with a preoperative neurological deficit, arterial reconstruction was performed in 18, which involved the common carotid in 17 patients and the internal, carotid in 1 patient. There was complete neurological recovery in 11 patients and 2 patients died. Four comatose patients had cerebral revascularization performed without fatality and with complete recovery in 3 of them. CT head scanning was not routinely employed for logistic reasons but has proved of limited value. No intraluminal arterial shunts were used in this series. It would seem that arterial reconstruction is not harmful and may well be beneficial to the young patient with extracranial cerebral arterial injury associated with a neurological deficit. Shunts are probably unnecessary for routine use.
Assuntos
Tronco Braquiocefálico/lesões , Lesões das Artérias Carótidas , Doenças do Sistema Nervoso/etiologia , Artéria Vertebral/lesões , Ferimentos Penetrantes/complicações , Adolescente , Adulto , Tronco Braquiocefálico/cirurgia , Artérias Carótidas/cirurgia , Coma/etiologia , Feminino , Humanos , Ligadura , Masculino , Métodos , Pessoa de Meia-Idade , Artéria Vertebral/cirurgia , Ferimentos Penetrantes/cirurgiaRESUMO
The surgical management of 2 patients presenting with symptomatic thoraco-abdominal aneurysms is reported. In the first patient a technique which utilized multiple side-arm grafts to the visceral arteries is described. In the second patient we used the simpler and more expeditious technique of anastomosing the visceral artery orifices to "windows" in the aortic replacement graft from within the opened aneurysm. The merits and demerits of the different techniques and their hazards are discussed. It would seem that few aneurysms can be regarded as inoperable.
Assuntos
Aneurisma Aórtico/cirurgia , Adulto , Aorta Abdominal/cirurgia , Aorta Torácica/cirurgia , Humanos , Masculino , Métodos , Pessoa de Meia-IdadeRESUMO
The initial clinical observations and methods and results of treatment in 104 patients with subclavian (48), vertebral (four), and carotid (52) artery injuries are reported. Delayed hemorrhage ten days after misdiagnosed subclavian artery injuries resulted in false aneurysms causing compressive brachial plexus palsies. A conservative approach to penetrating cervicomediastinal wounds was adopted with selective use of arch aortography when arterial injury was suspected by defined criteria. This proved safe, accurate, and invaluable for planning operative approach. Partial median sternotomy without entering the pleura proved optimal for superior mediastinal access; simple clavicle transection provided adequate distal subclavian exposure. External carotid and vertebral arteries were ligated. No shunts were employed for common and internal carotid repair. None of the 14 patients revascularized in the presence of a neurologic deficit died and none was made worse by carotid reconstruction.