Biofilm accumulation on additive manufactured Ti-6Al-4V alloy surfaces.

PURPOSE: This study investigated whether additive manufactured (AM) surfaces inhibit accumulation of bacterial biofilm on the surfaces of Ti-6Al-4V alloy dental implants. Bacterial biofilms are thought to cause peri-implant disease, which develops in mucosa surrounding titanium (Ti) and Ti alloy dental implants and can lead to bone loss and implant failure. METHODS: Accumulation of a Streptococcus mutans (ATCC 25175) biofilm on Ti-6Al-4V alloy was compared in relation to fabrication method, ie, AM using electron beam melting (EBM) or laser beam melting (LBM). Conventional lost-wax casting was used as positive control, and Teflon was used as negative control. Biofilm accumulation on the alloys and negative control (each n = 10) was conducted at 37°C under anaerobic conditions. After 4 h, the number of metabolically active S. mutans bacteria adhering to the alloy was determined with a bioluminescence assay. RESULTS: The quantitative roughness values of the specimens, before exposure to bacteria, ranked EBM > LBM > cast > Teflon. CONCLUSION: The amount of biofilm accumulation on the investigated AM metals and cast metal controls did not significantly differ.

Fabrication of titanium removable dental prosthesis frameworks with a 2-step investment coating method.

STATEMENT OF PROBLEM: Although pure titanium is known to have good biocompatibility, a titanium alloy with better strength is needed for fabricating clinically acceptable, partial removable dental prosthesis (RDP) frameworks. PURPOSE: The mechanical properties of an experimental Ti-5Al-5Cu alloy cast with a 2-step investment technique were examined for RDP framework applications. MATERIAL AND METHODS: Patterns for tests for various properties and denture frameworks for a preliminary trial casting were invested with a 2-step coating method using 2 types of mold materials: a less reactive spinel compound (Al(2)O(3)·MgO) and a less expensive SiO(2)-based material. The yield and tensile strength (n=5), modulus of elasticity (n=5), elongation (n=5), and hardness (n=8) of the cast Ti-5Al-5Cu alloy were determined. The external appearance and internal porosities of the preliminary trial castings of denture frameworks (n=2) were examined with a conventional dental radiographic unit. Cast Ti-6Al-4V alloy and commercially pure titanium (CP Ti) were used as controls. The data for the mechanical properties were statistically analyzed with 1-way ANOVA (α=.05). RESULTS: The yield strength of the cast Ti-5Al-5Cu alloy was 851 MPa and the hardness was 356 HV. These properties were comparable to those of the cast Ti-6Al-4V and were higher than those of CP Ti (P<.05). One of the acrylic resin-retention areas of the Ti-5Al-5Cu frameworks was found to have been incompletely cast. CONCLUSIONS: The cast biocompatible experimental Ti-5Al-5Cu alloy exhibited high strength when cast with a 2-step coating method. With a dedicated study to determine the effect of sprue design on the quality of castings, biocompatible Ti-5Al-5Cu RDP frameworks for a clinical trial can be produced.

Comparison of the flexural strength of five adhesive resin cements.

STATEMENT OF PROBLEM: The increased use of adhesive resin cements in bonded prosthetic restorations has led to restorations debonding under function. PURPOSE: This investigation evaluated the differences in the flexural strength of new adhesive resin cements as a function of specimen age and storage condition. MATERIALS AND METHODS: Four new dual-cure cements were compared to C/B Metabond. Twenty specimens of each of the five cements were prepared in a rectangular glass mold (25 x 2 x 2 mm). The new cements were light-activated with a 550 mW/cm(2) lamp for 80 seconds on both the top and bottom surfaces. The auto-cured cement was allowed to set according to manufacturer's directions. Half the specimens were tested immediately after curing while the other half were stored in distilled water at 37 degrees C for 30 days. A three-point bending test was performed using an Instron at a crosshead speed of 1 mm/min. The results were analyzed by analysis of variance and Scheffé tests ( p < 0.05) to examine the effect of specimen age and storage condition. RESULTS: RelyX ARC exhibited a significantly higher flexural strength compared with Calibra and Panavia F when tested immediately. The standard cement, C/B Metabond, deformed and did not fracture at the immediate test time. After storage, the flexural strength had significantly improved from the immediate test time for Calibra, Cement-It, Panavia F, and C/B Metabond. However, there were no significant differences in the flexural strength among the cements when tested after 30 days in water at 37 degrees C. CONCLUSION: Immediately after curing, these new adhesive resin cements are not equivalent, as evidenced by the significant variability in the measured flexural strength. The distinctions among the cements diminish after aging in water, which may be due to residual polymerization or a plasticizing effect from water absorption. CLINICAL SIGNIFICANCE: When light-cured, all the new adhesive resin cements have greater early strengths than the auto-cured cement; however, the wide variation in immediate bending strength suggests that some cements may be more appropriate for use in high-stress clinical situations such as resin-bonded fixed partial dentures.

Quality of removable partial dentures worn by the adult U.S. population.

STATEMENT OF PROBLEMS: Research-based assessments of the quality of removable partial dentures (RPDs), especially within a large patient population, are lacking. PURPOSE: This study evaluated the prevalence and quality of RPDs by use of the National Health and Nutrition Survey (NHANES III) data set. MATERIAL AND METHODS: Estimates of the health and nutritional status of the American population were obtained from the NHANES III data set (1988-1994). Calibrated dentists performed dental examinations on 17,884 adults. If the subject wore an RPD, its quality was assessed with 5 criteria: integrity, tooth wear, the presence of temporary reline material or adhesive, stability, and retention. In this study, the data on RPD defects were used to assess the prevalence of problems. The original population was divided into 4 subgroups: paired RPD data = subjects wearing both maxillary and mandibular RPDs (n = 600 prostheses, 300 maxillary and 300 mandibular); single RPD data = subjects with only one RPD (n = 1003 prostheses, 511 maxillary and 492 mandibular); maxillary versus mandibular RPD data = all subjects with all RPDs (n = 1603 prostheses, 811 maxillary and 792 mandibular); and pooled RPD data = all subjects with a single RPD plus subjects with 2 RPDs, counting only the RPD with the most defects (n = 1303 prostheses, 674 maxillary and 629 mandibular). The subjects were analyzed with descriptive statistics. The distribution of defects in the RPDs was examined for possible associations with chi(2) tests. The paired data for patients with both maxillary and mandibular RPDs were analyzed with Wilcoxon signed rank tests. The prevalence of RPDs as a function of patient age and the association between RPD defects and patient age were analyzed with chi(2) tests. The results were considered highly significant at P<.0001 and significant at P<.05. RESULTS: Of the 17,884 adults who underwent a dental examination, 1306 had RPDs. Three patients were excluded because their records were incomplete, leaving 1303 patients available for analysis. Most of the prostheses examined (65%) had at least 1 defect. Lack of stability was the most prevalent single defect. Distinctions in the type and prevalence of defects were observed between mandibular and maxillary RPDs. Mandibular RPDs had significantly more problems related to retention, whereas maxillary RPDs had significantly more problems related to the presence of reline material and to integrity defects. Tooth wear defects were significantly associated with patient age (P<.0001). Only one third of the RPDs were considered satisfactory according to NHANES III criteria. CONCLUSION: A review of the database used in this study indicated that, in spite of a decline in tooth loss, RPDs are still used in all age cohorts, including young adults. A large number of RPDs were found to have defects.