Elevated Tenascin-C Serum Levels in Patients With Axial Spondyloarthritis.

This study aimed to examine serum tenascin C (TNC) in different subsets of axial spondyloarthritis (axSpA) patients. Sixty-one patients fulfilling the Assessment of SpondyloArthritis international Society classification criteria for axSpA and 20 healthy subjects (HS) were included in study. Based on imaging, patients were classified as non-radiographic (n=16) and radiographic (n=45) axSpA. TNC serum levels were determined by ELISA. Disease-related factors including the Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) and C-reactive protein (CRP) levels, were determined. TNC levels were elevated in axSpA patients [535.3 (457.7-677.2) ng/ml] compared to HS [432.1 (329.1-565.9) ng/ml, p=0.007]. Dividing axSpA into radiographic and non-radiographic subsets, the difference in TNC was observed between the radiographic subset and HS [535.3 (434.5-677.2) vs. 432.1 (329.1-565.9) ng/ml, p=0.022]. TNC levels did not correlate with disease activity measures (serum CRP or BASDAI). Nevertheless, the weak correlation of TNC levels with different disease stages (r=0.25, p=0.025) was found, with the highest levels in patients with syndesmophytes. TNC levels are elevated across various subsets of axSpA, and although not related to systemic disease activity, TNC levels might reflect chronic structural spinal changes in axSpA patients. However, its specific role in bone metabolism should be elucidated in further studies.

[Distribution and binding of positional isomers of 125I-hippuran, 125I-benzoate and 125I-phenylacetate in the blood components of mice]. / Distribuce a vazba polohových izomeru 125I-hippuranu, 125I-benzoanu a 125I-fenyloctanu ve slozkách mysí krve.

On examining the dependencies between the parameters characterizing the structure and distribution of substances of acid character in the blood of experimental mice in the in vitro conditions it was demonstrated that both plasma and erythrocyte binding are structurally dependent. With increasing lipophilicity, the values of the drug fraction bound to proteins in the separated plasma, the values of the fraction bound to plasma proteins in the samples of whole blood, and the distribution into the erythrocytes in a suspension of erythrocytes are increased. With increasing lipophilicity, the distribution into the erythrocytes in the samples of the whole blood and the fraction in the plasma blood fluid are decreased.