Complete genome sequence of mcr-9 containing Leclercia adecarboxylata.

Here, we provide the genome sequence of a Leclercia adecarboxylata isolated from a screen of an environmental bacterial isolate library for resistance to the plant flavonoid berberine. We detected the colistin resistance gene mcr-9, located on an IncFII(pECLA) plasmid.

Functional and Sequence-Specific Screening Protocols for the Detection of Novel Antimicrobial Resistance Genes in Metagenomic DNA.

Antimicrobial resistance (AMR) is an increasingly important global challenge for healthcare systems as well as agricultural food production systems. Our ability to prepare for, and respond to, emerging AMR threats is dependent on our knowledge of genes able to confer AMR that are circulating within various environmental, animal, and human microbiomes. Targeted, sequence-specific, detection of AMR genes and functional resistance assays, described here, carried out on metagenomic DNA gives us unique insights into the presence of AMR genes and how these are associated with mobile genetic elements that may be responsible for their dissemination and can also provide important information about the mechanisms of resistance underpinning the phenotype.

Isolation of an antimicrobial-resistant, biofilm-forming, Klebsiella grimontii isolate from a reusable water bottle.

A reusable water bottle was swabbed as part of the citizen science project "Swab and Send," and a Klebsiella grimontii isolate was recovered on chromogenic agar and designated SS141. Whole-genome sequencing of SS141 showed it has the potential to be a human pathogen as it contains the biosynthetic gene cluster for the potent cytotoxin, kleboxymycin, and genes for other virulence factors. The genome also contains the antibiotic-resistant genes, blaOXY-6-4 , and a variant of fosA, which is likely to explain the observed resistance to ampicillin, amoxicillin, and fosfomycin. We have also shown that SS141 forms biofilms on both polystyrene and polypropylene surfaces, providing a reasonable explanation for its ability to colonize a reusable water bottle. With the increasing use of reusable water bottles as an alternative to disposables and a strong forecast for growth in this industry over the next decade, this study highlights the need for cleanliness comparable to other reusable culinary items.

Biomechanical Comparison of Fixed- versus Variable-Angle Locking Screws for Distal Humerus Comminuted Fractures.

BACKGROUND: To compare the stability of fixed- versus variable-angle locking constructs for the comminuted distal humerus fracture (AO/OTA 13-A3). METHODS: Eight pairs of complete humeri harvested from eight fresh frozen cadavers were used for the study. We fixed the intact humeri using 2.7-mm/3.5-mm locking VA-LCP stainless steel distal humerus posterolateral (nine-hole) and medial (seven-hole) plates. An oscillating saw was used to cut a 1-cm gap above the olecranon fossa. The specimens were loaded in axial mode with the rate of 1 mm per 10 seconds to failure, and stress-strain curves were compared in each pair. The mode of failure was recorded as well as the load needed for 2- and 4-mm displacement at the lateral end of the gap. RESULTS: The stiffness of the constructs, based on the slope of the stress-strain curve, did not show any difference between the fixed- versus variable-angle constructs. Likewise, there was no difference between the force needed for 2- or 4-mm displacement at the lateral gap between the fixed- and variable-angle constructs. The mode of failure was bending of both plates in all specimens and screw pull-out in four specimen pairs in addition to the plate bending. CONCLUSIONS: Our results did not show any difference in the biomechanical stability of the fixed- versus variable-angle constructs. There was not any screw breakage or failure of the plate-screw interface.

Biomechanical Evaluation of Standard Versus Extended Proximal Fixation Olecranon Plates for Fixation of Olecranon Fractures.

Background: Small olecranon fractures present a significant challenge for fixation, which has resulted in development of plates with proximal extension. Olecranon-specific plates with proximal extensions are widely thought to offer superior fixation of small proximal fragments but have distinct disadvantages: larger dissection, increased hardware prominence, and the increased possibility of impingement. Previous biomechanical studies of olecranon fracture fixation have compared methods of fracture fixation, but to date there have been no studies defining olecranon plate fixation strength for standard versus extended olecranon plates. The purpose of this study is to evaluate the biomechanical utility of the extended plate for treatment of olecranon fractures. Methods: Sixteen matched pairs of fresh-frozen human cadaveric elbows were used. Of the 16, 8 matched pairs received a transverse osteotomy including 25% and 8 including 50% of the articular surface on the proximal fragment. One elbow from each pair was randomly assigned to a standard-length plate, and the other elbow in the pair received the extended-length plate, for fixation of the fracture. The ulnae were cyclically loaded and subsequently loaded to failure, with ultimate load, number of cycles, and gap formation recorded. Results: There was no statistically significant difference between the standard and extended fixation plates in simple transverse fractures at either 25% or 50% from the proximal most portion of the articular surface of the olecranon. Conclusion: Standard fixation plates are sufficient for the fixation of small transverse fractures, but caution should be utilized particularly with comminution and nontransverse fracture patterns.

Biomechanical Strength of Retrograde Fixation in Proximal Third Scaphoid Fractures.

Background: Current techniques for fixation of proximal pole scaphoid fractures utilize antegrade fixation via a dorsal approach endangering the delicate vascular supply of the dorsal scaphoid. Volar and dorsal approaches demonstrate equivalent clinical outcomes in scaphoid wrist fractures, but no study has evaluated the biomechanical strength for fractures of the proximal pole. This study compares biomechanical strength of antegrade and retrograde fixation for fractures of the proximal pole of the scaphoid. Methods: A simulated proximal pole scaphoid fracture was produced in 22 matched cadaveric scaphoids, which were then assigned randomly to either antegrade or retrograde fixation with a cannulated headless compression screw. Cyclic loading and load to failure testing were performed and screw length, number of cycles, and maximum load sustained were recorded. Results: There were no significant differences in average screw length (25.5 mm vs 25.6 mm, P = .934), average number of cyclic loading cycles (3738 vs 3847, P = .552), average load to failure (348 N vs 371 N, P = .357), and number of catastrophic failures observed between the antegrade and retrograde fixation groups (3 in each). Practical equivalence between the 2 groups was calculated and the 2 groups were demonstrated to be practically equivalent (upper threshold P = .010). Conclusions: For this model of proximal pole scaphoid wrist fractures, antegrade and retrograde screw configuration have been proven to be equivalent in terms of biomechanical strength. With further clinical study, we hope surgeons will be able to make their decision for fixation technique based on approaches to bone grafting, concern for tenuous blood supply, and surgeon preference without fear of poor biomechanical properties.

Total Disk Replacement Adjacent to a Multilevel Fusion in the Cervical Spine: A Biomechanical Motion Analysis.

OBJECTIVE: Cervical total disk replacement (TDR) has emerged as a motion-preserving alternative to anterior cervical diskectomy fusion (ACDF). Biomechanical studies have demonstrated that the TDR preserves motion at the diseased segment and minimizes motion and stress at adjacent segments compared with fusion. There has been growing interest in performing a TDR adjacent to a cervical fusion. The purpose of this study was to investigate the kinematics of a TDR after sequentially fusing adjacent segments. METHODS: Seven fresh-frozen human cadaveric cervical spine specimens from C1-T1 were used (average age, 56.2 ± 7.3 years). The effect on cervical flexion-extension motion, by instrumenting a TDR above or below a 1-, 2-, or 3-level fusion, was measured. The protocol consisted of taking fluoroscopic images of each cervical specimen obtained at maximal angular displacement in flexion and extension during force application. Cobb angles were measured on digital radiographs to determine flexion-extension range of motion (ROM). RESULTS: Segmental ROM of the C6-7 TDR in the unfused spine was 11.3° ± 1.9°. After performing a 3-level fusion at C3-6, the motion of the C6-7 TDR increased to 12.9° ± 1.3° (P = 0.33). ROM of the C2-3 TDR in the unfused spine was 5.0° ± 1.1°. After performing a 3-level fusion of C3-6, the C2-3 TDR segmental motion was 6.1° ± 1.3° (P = 0.09). CONCLUSIONS: Biomechanically performing a cervical TDR adjacent to a long-segment fusion did not subject the implant to significantly greater motion than when the TDR was instrumented alone.

The combination of tibial anterior translation and axial rotation into a single biomechanical factor improves the prediction of patient satisfaction over each factor alone in patients with ACL reconstructed knees.

PURPOSE: The purpose of this study was to identify biomechanical factors, in both reconstructed and healthy knees, that correlate with patient satisfaction after ACL reconstruction. METHODS: Seventeen patients who had undergone unilateral ACL reconstruction were reviewed 9 years post-op. Patients completed subjective questionnaires and underwent manual knee laxity testing (Lachman-Trillat, KT-1000, and pivot shift) and automated laxity testing. During automated testing, both legs were rotated into external rotation and then internal rotation until peak rotational torque reached 5.65 Nm. Load-deformation curves were generated from torque and rotation data. Features of the curves were extracted for analysis. Total leg rotation and anterior laxity during KT-1000 testing were combined into a single factor (Joint Play Envelope or JPE). Patients were divided into groups based on patient satisfaction scores (Group 1: Higher Satisfaction, Group 2: Lower Satisfaction, Group 3: Unsatisfied). Load-deformation curve features and manual laxity testing results were compared between groups 1 and 2 to determine which biomechanical factors could distinguish between the groups. Diagnostic screening values were calculated for KT-1000 testing, the pivot shift test, total leg rotation and JPE. RESULTS: During manual testing, no significant differences in biomechanical factors were found when comparing reconstructed knees in group 1 and group 2. When comparing the reconstructed and healthy knees within group 2, the reconstructed knees had a significantly higher displacement during the KT-1000 manual maximum test (p < 0.002). When considering the reconstructed knees alone, neither the result of the pivot shift test nor KT-1000 testing could distinguish between group 1 and group 2. During automated testing, there were no significant differences between the groups when comparing the reconstructed lower limbs. The healthy lower limbs in group 2 had more maximum external rotation (p < 0.02) and decreased stiffness at maximum external rotation (p < 0.02) when compared to the healthy lower limbs in group 1. Total leg rotation was unable to distinguish between group 1 and group 2. JPE could distinguish between group 1 and group 2 when considering the reconstructed limb alone (p < 0.02). All four diagnostic screening values for JPE were equal or higher than in the other criteria. JPE also showed the most significant correlation with patient satisfaction. CONCLUSIONS: Joint Play Envelope is an objective measure that demonstrated improved predictive value as compared to other tests when used as a measure of satisfaction in patients with ACL reconstructed knees.

The effect of capacitively coupled (CC) electrical stimulation on human disc nucleus pulposus cells and the relationship between CC and BMP-7.

PURPOSE: An in vitro study using human intervertebral disc nucleus pulposus cells to evaluate the effects of CC stimulation on disc-matrix macromolecule production. METHODS: Nucleus pulposus cells were cultured in alginate beads and treated with CC stimulation. The effect of BMP on CC stimulation of the cells was evaluated by applying a BMP blocker (noggin) or by applying additional BMP-7 to the culture. The mRNA levels of the disc extracellular matrix genes (collagen I, II, aggrecan) and BMPs were measured by real-time PCR. The protein levels of aggrecan, collagen II, and BMPs were determined by ELISAs and Western blots. Sulfated glycosaminoglycan (sGAG) content was assayed using the DMMB method. RESULTS: (1) CC stimulation upregulates the production of the disc-matrix macromolecular components: sGAG, aggrecan and collagen II; (2) CC stimulation increases the ratio of mRNA expression levels of collagen II to collagen I; (3) CC stimulation induces the expression of endogenous BMP-4 and BMP-7; (4) inhibition of BMP activity (using noggin) reduces CC-mediated upregulation of aggrecan and collagen II; (5) CC and BMP-7 act in synergy to increase the upregulation of disc-matrix macromolecules. CONCLUSION: CC stimulation upregulates the production of the intervertebral disc-matrix macromolecules aggrecan, collagen II, and sGAG by a mechanism involving BMPs. CC stimulation acts in synergy with BMP-7 to increase the upregulation of these disc-matrix macromolecules.

Is It "In" or "Out"? The Optimal Fluoroscopic Views for Intraoperative Determination of Proper Lateral Mass Screw Placement.

STUDY DESIGN: Cadaveric. OBJECTIVE: Determine optimal fluoroscopic views for detecting cervical lateral mass screw (LMS) violations. SUMMARY OF BACKGROUND DATA: Single plane intraoperative x-rays are commonly used but frequently inadequate due to its complex trajectory. Fluoroscopy can be taken in multiple planes, but the ideal fluoroscopic view to assess malposition is not known: depending on the view, any given screw may look "in" or "out." METHODS: C3-6 LMS were inserted in three cadavers. To evaluate neuroforaminal violation, LMS were inserted into the foramen with the tip penetrating the anterior cortex by 0, 2, and 4 mm. To assess facet joint violation, LMS were inserted toward the subjacent facet joint with the tip penetrating the anterior cortex by 0 and 2 mm. Fluoroscopic views were taken 0°, 10°, 20°, 30°, and 40° to the lateral plane. Views were independently evaluated by three blinded spine surgeons. RESULTS: Twenty-degree oblique view correctly identified a 2 mm penetration into the neuroforamen in 79%, and a 4 mm penetration in 86%, for a sensitivity of 83% and specificity of 90%. Thirty-degree view had lower sensitivity (76%) but slightly higher specificity (93%). Twenty-degree and 30° views were significantly more sensitive than the other views. Zero-degree view correctly identified a 2 mm penetration into the facet joint in 93%, for a sensitivity of 93% and specificity of 92%. Ten-degree view had lower sensitivity (72%) but higher specificity (100%). The 0° view was significantly more sensitive than the other views. CONCLUSION: Twenty-degree and 30° oblique views significantly provided the most sensitive assessment of LMS potentially violating the neuroforamen, whereas the 0° neutral lateral view significantly provided the most sensitive assessment of facet violations. The specificities were also high (in the 90% range) for these views. We recommend the use of these views intraoperatively when assessing proper placement of LMS fluoroscopically. LEVEL OF EVIDENCE: N/A.

High-intensity stretch treatment for severe postoperative adhesive capsulitis of the shoulder.

BACKGROUND: Some patients with postoperative adhesive capsulitis reach a plateau in their recovery with a standard protocol of physical therapy (PT), which puts them at risk for further surgical intervention. OBJECTIVES: We aimed to evaluate therapy for postoperative adhesive capsulitis of the shoulder in 2 groups of patients: (1) those who used a high-intensity stretch (HIS) device after reaching a plateau in their recovery with a standard protocol of traditional PT (PT+HIS) and (2) those who showed no plateau in their recovery with a standard protocol of traditional PT alone (PT only). METHODS: We retrospectively reviewed the records for 60 patients (51 males; mean age 46.7±12.6years) with postoperative adhesive capsulitis who received treatment between March 2007 and May 2010. Forward elevation and combined internal/external rotation at the initial postoperative visit and final visit were measured. The measurements from group 2 patients were used as an observational benchmark. RESULTS: The PT+HIS (n=42) and PT-only (n=18) patients did not differ in total follow-up time. Initial elevation was worse for PT+HIS than PT-only patients (22.1° lower, P=0.02), but the final elevation was equivalent. Initial rotation was worse for PT+HIS than PT-only patients (16.6° lower, P=0.04), but the final rotation was higher for PT+HIS patients (10.6° higher, P=0.04). Gains in elevation and rotation were greater for the PT+HIS than PT-only patients (P=0.04 and P=0.01). CONCLUSIONS: Patients with postoperative adhesive capsulitis of the shoulder who are unable to reach their PT treatment goals with a standard protocol of PT may benefit from the addition of HIS to their treatment regimen. HIS could be a valuable adjunct to PT for treating postoperative adhesive capsulitis in appropriate patients.

Sagittal Plane Correction Using the Lateral Transpsoas Approach: A Biomechanical Study on the Effect of Cage Angle and Surgical Technique on Segmental Lordosis.

STUDY DESIGN: Cadaveric biomechanical study. OBJECTIVE: To determine the degree of segmental correction that can be achieved through lateral transpsoas approach by varying cage angle and adding anterior longitudinal ligament (ALL) release and posterior element resection. SUMMARY OF BACKGROUND DATA: Lordotic cage insertion through the lateral transpsoas approach is being used increasingly for restoration of sagittal alignment. However, the degree of correction achieved by varying cage angle and ALL release and posterior element resection is not well defined. METHODS: Thirteen lumbar motion segments between L1 and L5 were dissected into single motion segments. Segmental angles and disk heights were measured under both 50 N and 500 N compressive loads under the following conditions: intact specimen, discectomy (collapsed disk simulation), insertion of parallel cage, 10° cage, 30° cage with ALL release, 30° cage with ALL release and spinous process (SP) resection, 30° cage with ALL release, SP resection, facetectomy, and compression with pedicle screws. RESULTS: Segmental lordosis was not increased by either parallel or 10° cages as compared with intact disks, and contributed small amounts of lordosis when compared with the collapsed disk condition. Placement of 30° cages with ALL release increased segmental lordosis by 10.5°. Adding SP resection increased lordosis to 12.4°. Facetectomy and compression with pedicle screws further increased lordosis to approximately 26°. No interventions resulted in a decrease in either anterior or posterior disk height. CONCLUSION: Insertion of a parallel or 10° cage has little effect on lordosis. A 30° cage insertion with ALL release resulted in a modest increase in lordosis (10.5°). The addition of SP resection and facetectomy was needed to obtain a larger amount of correction (26°). None of the cages, including the 30° lordotic cage, caused a decrease in posterior disk height suggesting hyperlordotic cages do not cause foraminal stenosis. LEVEL OF EVIDENCE: N/A.

Biomechanical effects of rotator interval closure in shoulder arthroplasty.

BACKGROUND: Subscapularis dysfunction remains a significant problem after shoulder arthroplasty. Published techniques have variable recommendations for placing a rotator interval closing suture in attempts to off-load the subscapularis repair site, the implications of which have yet to be examined in the literature. The goals of this study were to investigate the biomechanical benefit of the rotator interval closing suture on the subscapularis repair strength and to analyze the effect on shoulder range of motion. METHODS: Sixteen matched cadaveric shoulders underwent a subscapularis tenotomy and shoulder arthroplasty. The subscapularis tenotomy was repaired, and motion at physiologic torsional force was recorded. One of each matched pair was randomly assigned to receive an additional rotator interval closure suture. Each specimen then underwent a standardized cyclic loading with measurement of gap formation and load to failure. RESULTS: The rotator interval closing suture significantly increased the ultimate load to failure of the subscapularis repair (452 N vs. 219 N; P = .002) and decreased gap formation at the subscapularis repair site. Measurement of the shoulder motion showed no significant difference between shoulders with and without the rotator interval closing suture. DISCUSSION: We report the additional biomechanical benefit that the rotator interval closing suture provides to the subscapularis repair site after shoulder arthroplasty. This suture acts to improve the load to failure of the subscapularis repair and to decrease gap formation under cyclic load. Furthermore, it does not detrimentally affect shoulder external rotation or overall arc of rotation. Our findings support the application of this off-loading technique after subscapularis repair during shoulder arthroplasty.

Improving Spanning-Knee External Fixator Stiffness: A Biomechanical Study.

The purpose of this study was to test and compare external fixator construct stiffness using pin-to-bar clamps or multipin clamps across 2 external fixation systems. Constructs were tested with 8-mm and 11-mm-diameter bar systems and pin-to-bar or multipin clamps. Three construct designs were tested: construct 1 with a single crossbar and pin-to-bar clamps, construct 2 with 2 crossbars and pin-to-bar clamps, and construct 3 with 2 crossbars and multipin clamps. The stiffness of each construct (N = 24) was tested using anterior-posterior bending. Two crossbars and pin-to-bar clamps resulted in the highest mean stiffness. Constructs with a single crossbar and pin-to-bar clamps had a similar average stiffness compared with constructs with 2 crossbars and multipin clamps. Pin-to-bar clamps with 2 crossbars result in stronger spanning-knee external fixators than constructs using multipin clamps.

Robotic axial lower leg testing: repeatability and reproducibility.

PURPOSE: The purpose of this study was to determine the test-retest reliability and the repeatability over multiple days of a robotic testing device when used to measure laxity of the lower leg during a simulated dial test. METHODS: Ten healthy subjects were evaluated using an instrumented robotic lower leg testing system over 4 days. Three testing cycles were performed each day. Each leg was rotated into external and then internal rotation by servomotors until a torque threshold of 5.65 N m was reached. Load-deformation curves were generated from torque and rotation data. Both average-measure and single-measure intraclass correlation coefficients (ICC) were compared across the curves. ICC scores were also compared for features of the curves including: maximum external rotation at -5.65 N m of torque, maximum internal rotation at 5.65 N m of torque, rotation at torque 0, compliance (slope of load-deformation curve) at torque 0, endpoint compliance in external rotation, endpoint compliance in internal rotation, and play at torque 0. Play at torque 0 was defined as the width of the hysteresis curve at torque 0. RESULTS: Average-measure ICC scores and test-retest scores were >0.95 along the entire load-deformation curve except around zero torque. ICC scores at maximum internal and external rotation ranged from 0.87 to 0.99 across the left and right knees. ICC scores for the other features of the curves ranged from 0.61 to 0.98. The standard error of the mean ranged from 0.0497 to 1.1712. CONCLUSIONS: The robotic testing device in this study proved to be reliable for testing a subject multiple times both within the same day and over multiple days. These findings suggest that the device can provide a level of reliability in rotational testing that allows for clinical use of test results. Objective laxity data can improve consistency and accuracy in diagnosing knee injuries and may enable more effective treatment.

A biomechanical comparison of 360° stabilizations for corpectomy and total spondylectomy: a cadaveric study in the thoracolumbar spine.

BACKGROUND: To date, there has been no adequate biomechanical model that would allow a quantitative comparison in terms of stability/stiffness between a corpectomy with the posterior column preserved and a total spondylectomy with the posterior column sacrificed. The objective of this study was to perform a biomechanical comparison of 360° stabilizations for corpectomy and total spondylectomy, using the human thoracolumbar spine. METHODS: Five human cadaveric thoracolumbar spines (T8-L2) were tested according to the following loading protocol: axial compression, flexion, extension, lateral bending to the right and left, and axial rotation to the right and left. This loading protocol was applied three times. Each specimen was tested intact, after corpectomy, and after total spondylectomy. The relative stiffness of each motion segment was determined for each test. RESULTS: There was no significant difference in stiffness after reconstruction of total spondylectomy versus corpectomy in our thoracolumbar model. Our construct consisted of an anterior cage and four-level pedicle screw instrumentation (two above and two below) and provided similar stiffness in both models. Despite the additional bone resection in a total spondylectomy versus corpectomy, the constructs did not differ biomechanically. Additionally, there was no significant difference in stiffness between the intact specimen and either reconstruction model. CONCLUSIONS: A classic corpectomy, which leaves the posterior column intact, is no better in terms of stability/stiffness than a total spondylectomy carried out using a shorter cage, followed by compression using posterior instrumentation.

The effect of hubbing on the pull-out strength of lateral mass screws in the cervical spine: a biomechanical experiment.

STUDY DESIGN: This was a cadaveric biomechanical experiment. OBJECTIVE: To compare the pull-out strength of polyaxial screws that are either hubbed or not hubbed when inserted into the lateral mass. SUMMARY OF BACKGROUND DATA: It has been shown in a study on pedicle screws in the thoracic spine that "hubbing" the head of the screw against the dorsal laminar cortex results in significantly lower pull-out strength of the screws. MATERIALS AND METHODS: Fifteen segments of the human cervical spine (from C3 to C7) were prepared. Polyaxial screws 3.5 mm in diameter were used. On one side screws 12 mm in length were inserted until the screw head touched the lateral mass; they were then turned 2.5 more times until they were fully hubbed (hubbed screws). On the other side screws 14 mm in length were inserted until the screw head just touched the lateral mass (nonhubbed screws). The 2 mm difference in length was to ensure that the screws were buried to the same length. All screws inserted into the lateral masses underwent tensile pull-out by applying a tensile force down the long axis of the screw. The difference in pull-out strength between the 2 groups was evaluated using a nonparametric paired test (the Wilcoxon signed rank test), which compared side to side on each vertebra. RESULTS: One specimen was excluded because of cement breakage during the biomechanical test. A total of 14 vertebrae were tested. Four vertebrae in the hubbed group showed small fractures or cracks around the screw hole after screw insertion. In a side to side comparison, the hubbed screws had significantly lower pull-out strengths as compared with the nonhubbed screws (P=0.033). CONCLUSIONS: Hubbing of lateral mass screws lowers the potential pull-out strength of the screws as compared with the pull-out strength of nonhubbed screws. Thus, hubbing of lateral mass screws, on the basis of the parameters applied in this study, is not recommended.

Two fixation methods for acromioclavicular joint reduction during coracoclavicular ligament reconstruction: a biomechanical analysis.

One specimen from each of six pairs of cadaveric shoulders underwent a semitendinosus coracoclavicular ligament reconstruction with a hook plate used for acromioclavicular joint reduction, while on the other specimen a polydioxanone (PDS) suture braid was utilized. Cyclical loading followed by maximal load-to-failure testing was performed. Displacement during cyclical loading, loads to 50% and 100% displacement, stiffness, and maximal load to failure were determined for all specimens. Results showed that the locking hook plate allowed significantly less displacement of the coracoclavicular interval during cyclical loading (3.41 vs. 9.67 mm, p = .0081) and withstood significantly higher loads before both 50% (225.5 vs. 107.7 N, p = .0197) and 100% displacement (410.6 vs. 240.1 N, p = .0077). The locking hook plate was found to be significantly stiffer than the PDS suture braid (28.2 vs. 18.4 N/mm, p = .0029), but there was no difference in maximal load to failure between the two fixation methods (hook plate, 434.4 N; PDS, 476.7 N; p = .76).

Comparison of screw trajectory on stability of oblique scaphoid fractures: a mechanical study.

PURPOSE: To determine whether a screw placed perpendicular to the fracture line in an oblique scaphoid fracture will provide fixation strength that is comparable with that of a centrally placed screw. METHODS: Oblique osteotomies were made along the dorsal sulcus of 8 matched pairs of cadaveric scaphoids. One scaphoid from each pair was randomized to receive a screw placed centrally down the long axis. In the other scaphoid, a screw was placed perpendicular to the osteotomy. Each scaphoid underwent cyclic loading from 80 N to 120 N at 1 Hz. Cyclic loading was carried out until 2 mm of fracture displacement occurred or 4,000 cycles was reached. The specimens that reached the 4,000-cycle limit were then loaded to failure. Screw length, number of cycles, and load to failure were compared between the groups. RESULTS: We found no difference in number of cycles or load to failure between the 2 groups. Screws placed perpendicular to the fracture line were significantly shorter than screws placed down the central axis. CONCLUSIONS: A perpendicularly placed screw provides equivalent strength to one placed along the central axis. CLINICAL RELEVANCE: Compared with a screw placed centrally in an oblique scaphoid fracture, a screw placed perpendicular to the fracture line allows the use of a shorter screw without sacrificing strength of fixation.

The effect of pedicle screw redirection after lateral wall breach--a biomechanical study using human lumbar vertebrae.

BACKGROUND CONTEXT: Currently, pedicle screw segmental fixation of the spine is considered a standard of care for a number of conditions. Most surgeons employ a free-hand technique using various intraoperative modalities to improve pedicle screw accuracy. Despite continued improvements in technique, pedicle breach remains a frequent occurrence. Once a breach is detected intraoperatively, the most common corrective maneuver is to medially redirect the pedicle screw into the pedicle. To our knowledge, the biomechanical impact of medially redirecting a pedicle screw after a lateral pedicle breach has not been examined. PURPOSE: To compare the fixation strength of perfectly placed pedicle screws to the fixation strength of pedicle screws that were correctly placed after having been redirected (RD) following a lateral pedicle breach. STUDY DESIGN/SETTING: A biomechanical study using human lumbar vertebrae. METHODS: Ten fresh human lumbar vertebrae were isolated from five donors. Each vertebra was instrumented with a monoaxial pedicle screw into each pedicle using two different techniques. On one side, a perfect center-center (CC) screw path was created using direct visualization and fluoroscopy. A 6.0-mm-diameter cannulated tap and a pedicle probe were used to develop the pedicle for the 7.0-mm-diameter by 45-mm-long cannulated pedicle screw, which was placed using a digital torque driver. On the contralateral side, an intentional lateral pedicle wall breach was created at the pedicle-vertebral body junction using a guide wire, a 6.0-mm-diameter cannulated tap, and a pedicle probe. This path was then redirected into a CC position, developed, and instrumented with a 7.0-mm-diameter by 45-mm-long cannulated pedicle screw: the RD screw. For each pedicle screw, we assessed four outcome measures: maximal torque, seating torque, screw loosening, and post-loosening axial pullout. Screw loosening and axial pullout were assessed using an MTS machine. RESULTS: The biomechanical cost of a lateral pedicle breach and the requirement to redirect the pedicle screw are as follows: an overall drop of 28% (p<.002) in maximal insertion torque and 25% (p<.049) in seating torque, a drop of 25% (p<.040) in resistance to screw loosening, and a drop in axial pullout force of 11% (p<.047). CONCLUSIONS: Compared with a CC lumbar pedicle screw, an RD lumbar pedicle screw placed after a lateral wall breach is significantly weaker in terms of maximal insertional torque, seating torque, screw loosening force, and axial pullout strength. These significant decreases in biomechanical properties are clearly important when RD pedicle screws are placed at the cephalad or caudal end of a long construct. In this situation, augmentation of the RD screw is an option.