RESUMO
Single-use filament columns for affinity chromatography are described. Antigenic filaments for the isolation of pure antibodies have proved to be much more effective than antibody filaments for the isolation of antigens. Nitrocellulose filter has been employed for the preparation of paper columns that may be used for the isolation of pure antibodies 8-10 times.
Assuntos
Cromatografia de Afinidade/instrumentação , Anticorpos/isolamento & purificação , Antígenos/isolamento & purificaçãoRESUMO
Standard pertussis agglutinative serum as well as antibodies to total and purified protective pertussis antigens, isolated from the serum by immunoadsorption technique, were studied. The treatment of donors with pertussis antibodies affected the T-suppressor formation, induced by high doses of sheep red blood cells, as was shown on the model of syngeneic transfer. Immunomodulating effect of antibodies, complementary to immunogen fraction of pertussis cells, was decreased. It was suggested that the observed antilymphocyte activity of antipertussis antibodies could play a certain role in postvaccination complications after corpuscular pertussis vaccine administration.
Assuntos
Anticorpos Antibacterianos/imunologia , Formação de Anticorpos , Bordetella pertussis/imunologia , Linfócitos T Reguladores/imunologia , Animais , Masculino , CamundongosRESUMO
The study of the physicochemical and immunobiological properties of B. pertussis dialysate antigen indicates that the antigen has a complex composition and possesses hemagglutinating and lymphocytosis-promoting activity, which permits further studies with a view to developing diagnostic and prophylactic preparations on the basis of this antigen.