Empirical approach to the gender gap in students' reading consumption in international contexts.

Reading is a very important aspect today, which is why it is a recurring topic in research. This study aims to analyze the gender gap in the reading consumption of adolescents and compare the situation in Spain with that of other countries. It addresses, for the first time, the influence of the gender factor on the reading habits of adolescents who have just begun secondary education. This is an empirical study carried out based on the research projects "Determining factors in the reading habits of secondary education students. A study from the variables of the educational context" and "Reading habits in international contexts of Secondary Education students. A study of educational practices to promote reading." It is based on the hypothesis that reading consumption is conditioned by gender and country-specific educational policies and, therefore, by the place where the study is carried out. To do this, an international sample of students was taken as a reference, made up of a matrix group of Spanish students contrasted with small samples from three different countries that we are interested in comparing with the Spanish context: Portugal, Poland, and Chile. The results have shown that the gender gap in reading is perceived in all the contexts studied and that it increases in contexts with less developed co-education programs; therefore, it is necessary to implement effective policies in the educational field to eliminate the existence of gender stereotypes.

A mutant pyruvate dehydrogenase E1 subunit allows survival of Escherichia coli strains defective in 1-deoxy-D-xylulose 5-phosphate synthase.

The 2-C-methyl-D-erythritol 4-phosphate pathway has been proposed as a promising target to develop new antimicrobial agents. However, spontaneous mutations in Escherichia coli were observed to rescue the otherwise lethal loss of the first two enzymes of the pathway, 1-deoxy-D-xylulose 5-phosphate (DXP) synthase (DXS) and DXP reductoisomerase (DXR), with a relatively high frequency. A mutation in the gene encoding the E1 subunit of the pyruvate dehydrogenase complex was shown to be sufficient to rescue the lack of DXS but not DXR in vivo, suggesting that the mutant enzyme likely allows the synthesis of DXP or an alternative substrate for DXR.

Identification of lethal mutations in Escherichia coli genes encoding enzymes of the methylerythritol phosphate pathway.

The recently elucidated methylerythritol phosphate (MEP) pathway for isoprenoid biosynthesis is essential in eubacteria (including Escherichia coli), the malaria parasite, and plants, but is absent in animals. Therefore, the pathway enzymes are promising targets for the development of novel herbicides and antimicrobials that are potentially innocuous for humans. For an effective drug design, it is important to identify the residues required to preserve the structure and activity of the MEP pathway enzymes. Here, we report a genetic approach to identify such residues in E. coli. A strain harboring a synthetic operon that allows the production of isoprenoids through a MEP-independent pathway was used to screen for the otherwise lethal loss-of-function point mutations in the MEP pathway genes generated by ethylmethane sulfonate (EMS) mutagenesis. Besides confirming the role of residues involved in catalysis, our results define regions within several of the proteins with a potential key role for enzyme function.