RESUMO
The main acidic glycosphingolipids (GSLs) of cock testis were identified as GalCer I3-sulfate and gangliosides GM4, GM3, GD3 and GT3. They contained N-acetylneuraminic acid as the major sialic acid, and ceramides composed mainly of sphingosine (dl8:1) and C18-24 non-hydroxy fatty acids. Appreciable amounts of hydroxy fatty acids were detected only in the GM4 preparation.
Assuntos
Glicoesfingolipídeos/análise , Testículo/química , Animais , Galinhas , Cromatografia em Camada Fina , Concentração de Íons de Hidrogênio , Espectroscopia de Ressonância Magnética , Masculino , Espectrometria de Massas de Bombardeamento Rápido de ÁtomosRESUMO
Ten days after inoculation of sarcomatous homogenate in the leg muscle of chickens, the appearance of sarcomatous tumours was observed by electron microscopy. Viral replication and cell transformation strongly altered phospholipids and glycolipids content of the tissue: 6.12 +/- 0.23 micromoles phospholipid phosphorus/g tissue in normal tissue and 3.5 +/- 0.19 micromoles phosphorus/g tissue in sarcomatous tissue. The concentration of lipid-bound sialic acids in normal tissues was 10.5 +/- 0 nmoles/g tissue and 24.27 +/- 0.8 nmoles/g tissue in sarcomatous tissue.