RESUMO
A 1-year-old male red kangaroo (Macropus rufus) with an acute clinical history of lethargy, depression and increased respiratory rate was presented for necropsy examination. Gross lesions in the digestive tract were a distended stomach with watery content and multifocal raised ulcers covered by fibrinous pseudomembranes in the forestomach and caecum. On histopathology, there was necrotizing and ulcerative gastritis and typhlitis with intralesional amoebic trophozoites and Toxoplasma gondii zoites. Lesions due to T. gondii infection were observed in multiple organs and diagnosis was confirmed by fluorescent antibody test and immunohistochemistry. Both toxoplasmosis and gastric amoebiasis are diseases described in macropods. In this case report, we describe concurrent disease caused by both protozoa in a red kangaroo. Lesions of amoebiasis were also observed in the caecum. Both toxoplasmosis and amoebiasis should be considered as differential diagnoses of ulcerative lesions in the gastrointestinal tract in macropods during necropsy examination. Amoebiasis should be suspected especially when ulcerative lesions are observed in the forestomach.
Assuntos
Coinfecção/veterinária , Entamebíase/veterinária , Trato Gastrointestinal/patologia , Macropodidae/parasitologia , Toxoplasmose Animal , Úlcera/veterinária , Animais , Animais de Zoológico , Coinfecção/parasitologia , Entamoeba histolytica/isolamento & purificação , Entamoeba histolytica/parasitologia , Entamebíase/diagnóstico , Entamebíase/patologia , Trato Gastrointestinal/parasitologia , Toxoplasma/isolamento & purificação , Toxoplasma/parasitologia , Toxoplasmose Animal/diagnóstico , Toxoplasmose Animal/patologiaRESUMO
A 2-tiered histologic grading scheme for canine cutaneous mast cell tumors (MCTs) is based on morphologic characteristics of neoplastic cells, including karyomegaly, multinucleation, nuclear pleomorphism, and mitotic figures. Aspirates from MCTs may provide the same information more quickly, inexpensively, and less invasively. This study used these criteria to develop a cytologic grading scheme for canine MCTs to predict outcome. Three anatomic pathologists graded histologic samples from 152 canine MCTs. Three clinical pathologists evaluated aspirates from these masses using similar criteria. A cytologic grading scheme was created based on correlation with histologic grade and evaluated with a kappa statistic. Survival was evaluated with Kaplan-Meier survival curves. Cox proportional hazards regression was used to estimate hazard ratios for tumor grades and individual grading components. Simple logistic regression tested for relationships between risk factors and mortality. The cytologic grading scheme that best correlated with histology (kappa = 0.725 ± 0.085) classified a tumor as high grade if it was poorly granulated or had at least 2 of 4 findings: mitotic figures, binucleated or multinucleated cells, nuclear pleomorphism, or >50% anisokaryosis. The cytologic grading scheme had 88% sensitivity and 94% specificity relative to histologic grading. Dogs with histologic and cytologic high grade MCTs were 39 times and 25 times more likely to die within the 2-year follow-up period, respectively, than dogs with low grade MCTs. High tumor grade was associated with increased probability of additional tumors or tumor regrowth. This study concluded that cytologic grade is a useful predictor for treatment planning and prognostication.
Assuntos
Doenças do Cão/patologia , Sarcoma de Mastócitos/veterinária , Neoplasias Cutâneas/veterinária , Animais , Doenças do Cão/diagnóstico , Doenças do Cão/mortalidade , Cães , Feminino , Masculino , Sarcoma de Mastócitos/diagnóstico , Sarcoma de Mastócitos/mortalidade , Sarcoma de Mastócitos/patologia , Gradação de Tumores/veterinária , Prognóstico , Pele/citologia , Pele/patologia , Neoplasias Cutâneas/diagnóstico , Neoplasias Cutâneas/mortalidade , Neoplasias Cutâneas/patologiaRESUMO
Gliomas are among the most common primary neural tumours of dogs. Cyclooxygenase-2 (COX-2) and c-kit overexpression are associated with increased aggressiveness of gliomas and decreased survival in human beings. COX-2 is the inducible form of cyclooxygenase, which catalyzes prostaglandin formation and may increase tumour proliferation and angiogenesis. C-kit is a tyrosine kinase receptor involved in normal cell physiology; c-kit is upregulated in some canine tumours. In this retrospective study, 20 canine gliomas were identified: 11 (55%) oligodendrogliomas, including 1 anaplastic variant; 1 (5%) oligoastrocytoma; and 8 (40%) astrocytomas, of which 2 were glioblastoma multiforme. None of the gliomas expressed COX-2. None of the gliomas were immunoreactive for c-kit, although all three high-grade tumours had intramural vascular expression. Consequently, COX-2 inhibitors would likely be ineffective against canine gliomas. C-kit inhibitors may have an anti-angiogenic effect in high-grade gliomas, but would likely be ineffective in low- and medium-grade tumours.
Assuntos
Neoplasias Encefálicas/veterinária , Ciclo-Oxigenase 2/metabolismo , Doenças do Cão/metabolismo , Regulação Neoplásica da Expressão Gênica/fisiologia , Glioma/veterinária , Proteínas Proto-Oncogênicas c-kit/metabolismo , Animais , Neoplasias Encefálicas/metabolismo , Neoplasias Encefálicas/patologia , Ciclo-Oxigenase 2/genética , Cães , Feminino , Glioma/metabolismo , Glioma/patologia , Masculino , Proteínas Proto-Oncogênicas c-kit/genética , Estudos RetrospectivosRESUMO
Thirty-two 4-month-old to 19-year-old female miniature pet pigs were spayed. Uterine lesions were present in all except 8 pigs. The 24 remaining pigs had diffuse cystic endometrial hyperplasia, of which 14 had smooth muscle tumors, including leiomyomas and leiomyosarcomas, in the uterus or broad ligament. Nodular endometrial lesions-including adenocarcinomas, adenomas, and/or adenomyosis-were present in 10 pigs, 3 of which had concurrent smooth muscle tumors. Pyometra was present in 3 pigs. In uterine sections with cystic endometrial hyperplasia, adenomyosis, or adenomas, approximately 70% of epithelial nuclei expressed estrogen receptor and progesterone receptor immunohistochemically; in adenocarcinomas, expression was 20%. Regardless of malignancy, more than 50% of nuclei in smooth muscle tumors expressed estrogen receptor and progesterone receptor. Aging was associated with the development of uterine lesions in miniature pet pigs.
Assuntos
Doenças dos Suínos/patologia , Porco Miniatura/anatomia & histologia , Doenças Uterinas/veterinária , Adenocarcinoma/patologia , Adenocarcinoma/veterinária , Adenoma/patologia , Adenoma/veterinária , Animais , Ligamento Largo/patologia , Hiperplasia Endometrial/patologia , Hiperplasia Endometrial/veterinária , Endométrio/patologia , Estrogênios/sangue , Feminino , Leiomioma/patologia , Leiomioma/veterinária , Leiomiossarcoma/patologia , Leiomiossarcoma/veterinária , Progesterona/sangue , Piometra/patologia , Piometra/veterinária , Suínos , Doenças dos Suínos/sangue , Doenças Uterinas/sangue , Doenças Uterinas/patologia , Neoplasias Uterinas/patologia , Neoplasias Uterinas/veterinária , Útero/patologiaRESUMO
A 39-yr-old wild-caught, female western lowland gorilla (Gorilla gorilla gorilla) died during an immobilization to assess swelling and apparent pain of the cervical region. Necropsy revealed a fistulous tract containing plant material in the oropharynx, above the soft palate, communicating with a left-sided cervical necrotizing fasciitis and myositis. Alpha-hemolytic Streptococcus and Prevotella sp. were isolated from the cervical lesion. This is a report of cervical necrotizing fasciitis in a western lowland gorilla.
Assuntos
Doenças dos Símios Antropoides/patologia , Infecções por Bacteroidaceae/veterinária , Fasciite Necrosante/veterinária , Gorilla gorilla , Miosite/veterinária , Pescoço/patologia , Infecções Estreptocócicas/veterinária , Animais , Infecções por Bacteroidaceae/patologia , Fasciite Necrosante/patologia , Evolução Fatal , Feminino , Miosite/patologia , Prevotella , Infecções Estreptocócicas/patologia , StreptococcusRESUMO
A sporadic wasting syndrome affecting beef cattle herds parasitized by Eurytrema coelomaticum is described in the State of Paraná, southern Brazil. The disease was characterized by progressive weight loss, poor body condition despite plenty of good quality forage available, and death. Annual losses ranged from 1 to 3%. The clinical course of the disease varied from 2 to 10 months after the onset of the first clinical signs. At necropsy, one of the three distinct following patterns of lesions were observed: the pancreas was of normal size and color; small, shrunken, white and markedly and diffusely firm (fibrosis); or slightly enlarged and dark with a shriveled capsular surface. Myriads of leaf-shaped trematodes of the genus Eurytrema were packed inside multiple dilated ducts with thickened, whitish fibrous walls, or the flukes were embedded in the remaining pancreatic parenchyma. Microscopic findings included extensive loss of the pancreatic parenchyma with replacement fibrosis, intralesional flukes and eggs, and ductal hyperplasia. Inflammatory reaction varied from absent to severe with the presence of a granulomatous reaction around the trematode eggs. One affected animal had high plasma amylase concentration (1580U/L) suggesting exocrine pancreatic insufficiency. Glucose blood levels were not significantly increased. We suggest that diffuse and marked fibrosis of the pancreas induced by the presence of large amounts of pancreatic flukes is the cause of the sporadic cases of chronic wasting and death of cattle in this geographic area.
Assuntos
Doenças dos Bovinos/parasitologia , Pancreatopatias/veterinária , Trematódeos/crescimento & desenvolvimento , Infecções por Trematódeos/veterinária , Síndrome de Emaciação/veterinária , Animais , Brasil/epidemiologia , Bovinos , Doenças dos Bovinos/epidemiologia , Doenças dos Bovinos/patologia , Histocitoquímica/veterinária , Masculino , Pancreatopatias/epidemiologia , Pancreatopatias/parasitologia , Pancreatopatias/patologia , Estudos Prospectivos , Estudos Retrospectivos , Infecções por Trematódeos/epidemiologia , Infecções por Trematódeos/parasitologia , Infecções por Trematódeos/patologia , Síndrome de Emaciação/epidemiologia , Síndrome de Emaciação/parasitologia , Síndrome de Emaciação/patologiaRESUMO
A 15-year-old female Holstein cow was presented with a history of anorexia and weight loss. Abnormal physical examination findings included poor body condition, dehydration, icterus, and photodermatitis on nonpigmented areas of the skin of the dorsum. At necropsy, the common bile duct was greatly enlarged, with thickened walls, and tightly adhered to the hepatic capsular surface and serosa of adjacent loops of the small intestine. Two sessile, yellowish, friable, well-circumscribed, cauliflower-like, bulging masses (14 and 8 cm in diameter) were attached to the inner surface of the common bile duct blocking its lumen. Microscopically, the tumor was composed of well-differentiated, columnar epithelial cells with oval or round, vesicular, basal nuclei arranged in papillary projections. Periodic acid-Schiff-positive material accumulated in the apical cytoplasm of the neoplastic cells. Immunostaining of the tumor cells was positive for cytokeratin (CK) of wide-spectrum screening and for CK 7. The diagnosis of papillary adenocarcinoma of the extrahepatic bile duct was based on the morphologic features of the neoplasm and evidence of local invasion. To the best of our knowledge, extrahepatic bile duct carcinomas have not been described in cattle.
Assuntos
Adenocarcinoma Papilar/veterinária , Neoplasias dos Ductos Biliares/veterinária , Ductos Biliares Extra-Hepáticos/patologia , Doenças dos Bovinos/patologia , Adenocarcinoma Papilar/metabolismo , Adenocarcinoma Papilar/patologia , Animais , Neoplasias dos Ductos Biliares/metabolismo , Neoplasias dos Ductos Biliares/patologia , Bovinos , Doenças dos Bovinos/metabolismo , Evolução Fatal , Feminino , Histocitoquímica/veterinária , Queratina-7 , Queratinas/metabolismoRESUMO
Descrevem-se seis casos de alteração fibroadenomatosa mamária felina (AFAMF) associada à administração de uma única injeção de acetato de medroxiprogesterona (AMP) na dose recomendada pelos fabricantes. A doença foi observada em gatas jovens não-castradas atendidas em dois hospitais veterinários de 1999 a 2001. O diagnóstico de AFAMF foi feito com base no histórico, sinais clínicos e achados macroscópicos e confirmado histologicamente em quatro dos seis casos. Sugere-se que a ocorrência da AFAMF esteja associada ao efeito prolongado de uma única injeção de AMP de depósito administrada em situações nas quais o emprego dessa terapia não é recomendado.