Anatomical and Physiological Changes Following Primary Palatoplasty Using "The Buccal Flap Approach".

OBJECTIVE: The purpose of this study was to determine the anatomical differences among selected individuals with a cleft palate repaired using "The Buccal Flap Approach" during primary palatoplasty compared to aged-matched participants without cleft palate. DESIGN: Observational, prospective. SETTING: Two regional hospitals. PARTICIPANTS: A total of 30 adult males consisting of 15 adults born with cleft palate who received the Double Opposing Z-Plasty plus Buccal Flaps (DOZP + BF) repair at the time of primary palatoplasty and no history of secondary speech surgery or orthognathic surgery and 15 adults without a history of cleft palate. INTERVENTIONS: All participants underwent MRI to visualize anatomy. MAIN OUTCOME MEASURES: Ten velopharyngeal and craniofacial anatomical measures. RESULTS: No statistically significant differences between groups were observed for velar thickness, velar length, pharyngeal depth, NSBa angle, SNB angle, or levator veli palatini length. Individuals with the DOZP + BF presented with a greater effective velar length (p < .001), greater effective VP ratio (p < .001), smaller SNA angle (p < .001), and smaller maximal velar stretch (p < .001) compared to the control participants. CONCLUSIONS: This study suggests that adult males who received the DOZP + BF repair at the time of primary palatoplasty and no history of secondary speech surgery or orthognathic surgery present with a longer effective velar length and larger effective VP ratio in comparison to the non-cleft group. Future research is needed to compare patients with and without favorable outcomes from multiple surgical types to fully understand how surgical techniques alter the anatomy.

Sex Differences in Velopharyngeal Anatomy of 9- and 10-Year-Old Children.

OBJECTIVE: Understanding the normal anatomy of velopharyngeal (VP) mechanism and the emergence of sexual dimorphism provides valuable insights into differences of VP anatomy among males and females. The purpose of this study is to examine sex differences in VP anatomy in a large data set of 3,248 9- and 10-year-old children. METHOD: Static three-dimensional magnetic resonance imaging was used to compare five VP characteristics including velar length, velar thickness, effective velar length, levator veli palatini muscle length, and pharyngeal depth between age-matched males (n = 1,670) and females (n = 1,578). Additionally, these dimensions were used to determine the VP ratio and effective VP ratio. RESULTS: Males showed significantly larger dimensions for all VP distances and significantly lower ratios of velar length and effective velar length to pharyngeal depth (p < .05). The magnitude of these effect sizes was small to medium, with Cohen's d values ranging from 0.12 to 0.63. Additionally, the VP ratio and effective VP ratio are lower among males compared to females (p < .05). CONCLUSIONS: Results suggest the presence of sexual dimorphism in the VP mechanism among 9- and 10-year-old children. These findings emphasize the necessity of using different normative data for males and females when making comparisons to patients with cleft palate.

Preliminary Development of an MRI Atlas for Application to Cleft Care: Findings and Future Recommendations.

OBJECTIVE: To introduce a highly innovative imaging method to study the complex velopharyngeal (VP) system and introduce the potential future clinical applications of a VP atlas in cleft care. DESIGN: Four healthy adults participated in a 20-min dynamic magnetic resonance imaging scan that included a high-resolution T2-weighted turbo-spin-echo 3D structural scan and five custom dynamic speech imaging scans. Subjects repeated a variety of phrases when in the scanner as real-time audio was captured. SETTING: Multisite institution and clinical setting. PARTICIPANTS: Four adult subjects with normal anatomy were recruited for this study. MAIN OUTCOME: Establishment of 4-D atlas constructed from dynamic VP MRI data. RESULTS: Three-dimensional dynamic magnetic resonance imaging was successfully used to obtain high quality dynamic speech scans in an adult population. Scans were able to be re-sliced in various imaging planes. Subject-specific MR data were then reconstructed and time-aligned to create a velopharyngeal atlas representing the averaged physiological movements across the four subjects. CONCLUSIONS: The current preliminary study examined the feasibility of developing a VP atlas for potential clinical applications in cleft care. Our results indicate excellent potential for the development and use of a VP atlas for assessing VP physiology during speech.

Enhancing linguistic research through 2-mm isotropic 3D dynamic speech MRI optimized by sparse temporal sampling and low-rank reconstruction.

PURPOSE: To enable a more comprehensive view of articulations during speech through near-isotropic 3D dynamic MRI with high spatiotemporal resolution and large vocal-tract coverage. METHODS: Using partial separability model-based low-rank reconstruction coupled with a sparse acquisition of both spatial and temporal models, we are able to achieve near-isotropic resolution 3D imaging with a high frame rate. The total acquisition time of the speech acquisition is shortened by introducing a sparse temporal sampling that interleaves one temporal navigator with four randomized phase and slice-encoded imaging samples. Memory and computation time are improved through compressing coils based on the region of interest for low-rank constrained reconstruction with an edge-preserving spatial penalty. RESULTS: The proposed method has been evaluated through experiments on several speech samples, including a standard reading passage. A near-isotropic 1.875 × 1.875 × 2 mm3 spatial resolution, 64-mm through-plane coverage, and a 35.6-fps temporal resolution are achieved. Investigations and analysis on specific speech samples support novel insights into nonsymmetric tongue movement, velum raising, and coarticulation events with adequate visualization of rapid articulatory movements. CONCLUSION: Three-dimensional dynamic images of the vocal tract structures during speech with high spatiotemporal resolution and axial coverage is capable of enhancing linguistic research, enabling visualization of soft tissue motions that are not possible with other modalities.

Establishing a Clinical Protocol for Velopharyngeal MRI and Interpreting Imaging Findings.

Traditional imaging modalities used to assess velopharyngeal insufficiency (VPI) do not allow for direct visualization of underlying velopharyngeal (VP) structures and musculature which could impact surgical planning. This limitation can be overcome via structural magnetic resonance imaging (MRI), the only current imaging tool that provides direct visualization of salient VP structures. MRI has been used extensively in research; however, it has had limited clinical use. Factors that restrict clinical use of VP MRI include limited access to optimized VP MRI protocols and uncertainty regarding how to interpret VP MRI findings. The purpose of this paper is to outline a framework for establishing a novel VP MRI scan protocol and to detail the process of interpreting scans of the velopharynx at rest and during speech tasks. Additionally, this paper includes common scan parameters needed to allow for visualization of velopharynx and techniques for the elicitation of speech during scans.

4D magnetic resonance imaging atlas construction using temporally aligned audio waveforms in speech.

Magnetic resonance (MR) imaging is becoming an established tool in capturing articulatory and physiological motion of the structures and muscles throughout the vocal tract and enabling visual and quantitative assessment of real-time speech activities. Although motion capture speed has been regularly improved by the continual developments in high-speed MR technology, quantitative analysis of multi-subject group data remains challenging due to variations in speaking rate and imaging time among different subjects. In this paper, a workflow of post-processing methods that matches different MR image datasets within a study group is proposed. Each subject's recorded audio waveform during speech is used to extract temporal domain information and generate temporal alignment mappings from their matching pattern. The corresponding image data are resampled by deformable registration and interpolation of the deformation fields, achieving inter-subject temporal alignment between image sequences. A four-dimensional dynamic MR speech atlas is constructed using aligned volumes from four human subjects. Similarity tests between subject and target domains using the squared error, cross correlation, and mutual information measures all show an overall score increase after spatiotemporal alignment. The amount of image variability in atlas construction is reduced, indicating a quality increase in the multi-subject data for groupwise quantitative analysis.

5,7,3',4'-Hydroxy substituted flavonoids reduce the heme of cytochrome c with a range of rate constants.

Flavonoids are antioxidants known to be abundant in edible plants. Seven 5,7,3',4'-tetrahydroxy substituted flavonoids representing each major flavonoid class were used as cytochrome c reductants to systematically investigate the redox role of their C-rings. Additional examples of flavonoids and benzenediols were investigated to better understand the role of the B-ring. Pseudo-first order rate constants (kobs) and apparent bimolecular rate constants (kapp) values were calculated from spectroscopic measurements. Of the seven flavonoids tested, five yielded measurable observed reduction rate constants. Butein (a chalcone) had the highest apparent bimolecular rate constant (kapp), followed by taxifolin (a flavanonol), catechin (a flavanol), eriodictyol (a flavanone), and luteolin (a flavone). Quercetin (a flavonol) and cyanidin (an anthocyanidin), however, reduced cytochrome c but kapp rate constants were unable to be calculated. Neither this trend nor trends in observed rate constants correlated with flavonoid pKa, solvent accessible surface area, polar surface area, reduction potential, antioxidant ability, resonance, or radical scavenging efficiency. Weak correlation, however, was found with degrees of freedom and the number of redox involved electrons. While some cytochrome c reduction rates have been reported, this study is the first to systematically investigate the role of the structure of the flavonoid C-ring across a full set of flavonoids with identical B-rings.

Polyurethane foam/nano hydroxyapatite composite as a suitable scaffold for bone tissue regeneration.

In bone tissue regeneration, the use of biomineralized scaffolds to create the 3D porous structure needed for well-fitting with defect size and appropriate cell interactions, is a promising alternative to autologous and heterologous bone grafts. Biomineralized polyurethane (PU) foams are here investigated as scaffold for bone tissue regeneration. Biomineralization of the foams was carried out by activation of PU surface by a two steps procedure performed for different times (1 to 4 weeks). Scaffolds were investigated for morphological, chemico-physical and mechanical properties, as well as for in vitro interaction with rat Bone Marrow Mesenchymal Stem Cells (BMSCs). Untreated and biomineralized PU samples showed a homogenous morphology and regular pore size (average Ø=407µm). Phase and structure of formed calcium phosphates (CaPs) layer onto the PU foam were analyzed by Fourier Transform Infrared spectroscopy and X-ray diffraction, proving the formation of bone-like nano hydroxyapatite. Biomineralization caused a significant increase of mechanical properties of treated foams compared to untreated ones. Biomineralization also affected the PU scaffold cytocompatibility providing a more appropriate surface for cell attachment and proliferation. Considering the obtained results, the proposed scaffold can be considered suitable for bone tissue regeneration.

Seroprevalence of HBV, HCV and HIV infection among intravenous drug users in Shahr-e-Kord, Islamic Republic of Iran.

This study was conducted in 2004 to evaluate the prevalence of hepatitis B (HBV), hepatitis C (HBC) and human immunodeficiency (HIV) virus infection in 133 injecting drug users (IDUs) attending a voluntary rehabilitation centre in Shahr-e-Kord, and to identify risk factors for these infections in this group. Serum samples were screened for HBV, HCV and HIV infection. Only 1 participant (0.8%) was HIV positive, 15 (11.2%) were HCV positive and 8 (6.0%) were positive for HBV surface antigen. There was significant correlation between using shared syringes and infection with HIV, HCV and HBV.

Seroprevalence of HBV, HCV and HIV infection among intravenous drug users in Shahr-e-Kord, Islamic Republic of Iran

This study was conducted in 2004 to evaluate the prevalence of hepatitis B [HBV], hepatitis C [HBC] and human immunodeficiency [HIV] virus infection in 133 injecting drug users [IDUs] attending a voluntary rehabilitation centre in Shahr-e-Kord, and to identify risk factors for these infections in this group. Serum samples were screened for HBV, HCV and HIV infection. Only 1 participant [0.8%] was HIV positive, 15 [11.2%] were HCV positive and 8 [6.0%] were positive for HBV surface antigen. There was significant correlation between using shared syringes and infection with HIV, HCV and HBV