1.
Appl Environ Microbiol
; 75(20): 6613-5, 2009 Oct.
Artigo
em Inglês
| MEDLINE
| ID: mdl-19684165
RESUMO
We have developed a stable isopropyl-beta-d-thiogalactopyranoside (IPTG)-inducible-expression plasmid, pIND4, which allows graduated levels of protein expression in the alphaproteobacteria Rhodobacter sphaeroides and Paracoccus denitrificans. pIND4 confers kanamycin resistance and combines the stable replicon of pMG160 with the lacI(q) gene from pYanni3 and the lac promoter, P(A1/04/03), from pJBA24.
