RESUMO
Herbicide use, mainly glyphosate, has been intense in worldwide agriculture, including in the Brazilian Amazon region. This study aimed to validate a method for determining glyphosate and its degradation product, AMPA, and glufosinate by HPLC-FL in 58 water samples collected at the Santarém plateau region (Planalto Santareno), in the western of Pará state, Brazil. The method involves filtration and direct injection in the HPLC-FL for AMPA analysis, or previous concentration (10×) by lyophilization for glufosinate and glyphosate analysis. Analytes were oxidized and complexed with o-phthalaldehyde and 2-mercaptoethanol in a post-column reaction before fluorescence detection. LOQs for AMPA, glyphosate and glufosinate were established at 0.5, 0.2 and 0.3 µg L-1, respectively. A total of 58 samples were collected. Glyphosate and glufosinate were not detected in any of the 30 surface water samples collected in 2015 (Assuntos
Aminobutiratos/análise
, Cromatografia Líquida de Alta Pressão/métodos
, Glicina/análogos & derivados
, Poluentes Químicos da Água/análise
, Água/química
, Ácido alfa-Amino-3-hidroxi-5-metil-4-isoxazol Propiônico/análise
, Brasil
, Monitoramento Ambiental
, Fluorescência
, Liofilização
, Glicina/análise
, Herbicidas/análise
, Limite de Detecção
, Reprodutibilidade dos Testes
, Glifosato