Distinct cell proliferation patterns underlying the development of defensive crests in Daphnia longicephala.

The freshwater crustacean Daphnia is well known for its expression of morphological defenses in the presence of predators. Research into this phenomenon has mostly centered on the ecology and evolution of Daphnia defenses; information is limited on the cellular mechanisms that underlie site-specific tissue growth. We aimed to determine these cellular mechanisms, specifically those associated with the development of defensive crests in D. longicephala. With the help of a cell-proliferation assay we monitored changes in the epidermal tissue of naïve and predator-exposed D. longicephala. Based on our results, we propose that cell division is delayed in favor of cell growth, which results in crest formation. Further, we identify specific regions of proliferative activity in a time-dependent manner. Defense development starts in the ventral region, before extending in the cranial and then dorsal directions. We demonstrate that these cellular changes begin as early as 2 h after predator exposure. Our results provide new insights into the cellular processes underlying morphological defense expression in Daphnia.

Daphnia longicephala neuropeptides: morphological description of crustacean cardioactive peptide (CCAP) and periviscerokinins in the Ctenodaphnia central nervous system.

The publication of the Daphnia genome has driven research in this ecologically relevant model organism in many directions. However, information on this organism's physiology and the relevant controlling factors is limited. In this regard, especially neuropeptides are important biochemical regulators that control a variety of cellular processes, which in combination influence physiological conditions and allow the adaptation of the internal physiological state to external conditions. Thus, neuropeptides are prime in understanding an organism's physiology. We here aimed to detect and describe the distribution of evolutionary conserved neuropeptides including the crustacean cardioactive peptide (CCAP) and peptides of the family periviscerokinins (PVKs) in the central nervous system and the periphery of the Daphnia longicephala head region. We were able to identify a large pair of CCAP immunoreactive cells within central nervous system. In addition, in the periphery we found CCAP immunoreactive cells in the epidermis of the head with processes indicating cuticular secretion. Furthermore, we were able to identify and describe a complex neuronal circuit of PVK neuropeptides in the central nervous system. The data obtained in this study will provide important background information for future investigations aiming to unravel the cellular, neuronal and physiological pathways in a highly adaptive organism such as Daphnia.