1.
Genetika
; 24(4): 602-12, 1988 Apr.
Artigo
em Russo
| MEDLINE
| ID: mdl-2840339
RESUMO
43 cDNA clones specific for murine cellular tumor antigen p53 were isolated from a library constructed using 17S fraction of mRNA from the SV40 transformed murine fibroblasts (SVT2). These clones contain the whole coding region of p53 mRNA and the most of non-translated sequences. A plasmid containing 1.8 kb insert of p53 cDNA was constructed. The p53 specific insert in this plasmid was colinear with p53 mRNA, as revealed by S1 nuclease analysis. 5'-region of the p53 gene comprising non-translated and promoter areas was cloned from the mouse genomic library. A combined clone containing promoter and the whole region, corresponding to p53 mRNA has been constructed.
Assuntos
DNA/genética , Proteínas de Neoplasias/genética , Fosfoproteínas/genética , RNA Mensageiro/isolamento & purificação , Animais , Linhagem Celular Transformada , Mapeamento Cromossômico , Clonagem Molecular , Enzimas de Restrição do DNA , Fibroblastos , Camundongos , Hibridização de Ácido Nucleico , RNA Mensageiro/genética , Vírus 40 dos Símios , Proteína Supressora de Tumor p53
2.
Dokl Akad Nauk SSSR
; 267(5): 1272-5, 1982.
Artigo
em Russo
| MEDLINE
| ID: mdl-6295732