Pseudo-fermion functional renormalization group for spin models.

For decades, frustrated quantum magnets have been a seed for scientific progress and innovation in condensed matter. As much as the numerical tools for low-dimensional quantum magnetism have thrived and improved in recent years due to breakthroughs inspired by quantum information and quantum computation, higher-dimensional quantum magnetism can be considered as the final frontier, where strong quantum entanglement, multiple ordering channels, and manifold ways of paramagnetism culminate. At the same time, efforts in crystal synthesis have induced a significant increase in the number of tangible frustrated magnets which are generically three-dimensional in nature, creating an urgent need for quantitative theoretical modeling. We review the pseudo-fermion (PF) and pseudo-Majorana (PM) functional renormalization group (FRG) and their specific ability to address higher-dimensional frustrated quantum magnetism. First developed more than a decade ago, the PFFRG interprets a Heisenberg model Hamiltonian in terms of Abrikosov pseudofermions, which is then treated in a diagrammatic resummation scheme formulated as a renormalization group flow ofm-particle pseudofermion vertices. The article reviews the state of the art of PFFRG and PMFRG and discusses their application to exemplary domains of frustrated magnetism, but most importantly, it makes the algorithmic and implementation details of these methods accessible to everyone. By thus lowering the entry barrier to their application, we hope that this review will contribute towards establishing PFFRG and PMFRG as the numerical methods for addressing frustrated quantum magnetism in higher spatial dimensions.

Recent advances in chitosan-based materials; The synthesis, modifications and biomedical applications.

The attention to polymer-based biomaterials, for instance, chitosan and its derivatives, as well as the techniques for using them in numerous scientific domains, is continuously rising. Chitosan is a decomposable naturally occurring polymeric material that is mostly obtained from seafood waste. Because of its special ecofriendly, biocompatible, non- toxic nature as well as antimicrobial properties, chitosan-based materials have received a lot of interest in the field of biomedical applications. The reactivity of chitosan is mainly because of the amino and hydroxyl groups in its composition, which makes it further fascinating for various uses, including biosensing, textile finishing, antimicrobial wound dressing, tissue engineering, bioimaging, gene, DNA and drug delivery and as a coating material for medical implants. This study is an overview of the different types of chitosan-based materials which now a days have been fabricated by applying different techniques and modifications that include etherification, esterification, crosslinking, graft copolymerization and o-acetylation etc. for hydroxyl groups' processes and acetylation, quaternization, Schiff's base reaction, and grafting for amino groups' reactions. Furthermore, this overview summarizes the literature from recent years related to the important applications of chitosan-based materials (i.e., thin films, nanocomposites or nanoparticles, sponges and hydrogels) in different biomedical applications.

Environmental MCS package and green intellectual capital influence environmental performance: a mediated-moderated perspective.

Nowadays, business is not only a financial entity. The environmental impact of rapid business growth necessitates solutions. This study considers natural resource orchestration theory (ROT) to study the impact of the environment management control system (MCS) package and green intellectual capital (IC) on environmental performance through the mediating role of top management support (TMS) and the interaction role of green business strategies. This study employed partial least squares structural equation modeling to analyze 358 questionnaires. Simple sampling was used to collect data from Pakistan manufacturing firms. The results revealed that the environmental MCS package significantly influences environmental performance directly and through TMS mediation. Still, the green IC does not influence environmental performance directly or through mediation in selected industries of Pakistan. Furthermore, the interaction of TMS and green business strategies significantly determines environmental performance. This study suggests managers use the environmental MCS package, green IC, top management support, and green business strategies in decision-making to determine environmental performance of manufacturing firms in Pakistan even though green IC has no influence on environmental performance. This study used natural ROT to impart the managerial role along with bundling firms' resources and strategies (i.e., environmental MCS package, green IC, top management support, and green business strategies) which is ignored in the resource-based view.

Quantum Effects on Unconventional Pinch Point Singularities.

Fracton phases are a particularly exotic type of quantum spin liquids where the elementary quasiparticles are intrinsically immobile. These phases may be described by unconventional gauge theories known as tensor or multipolar gauge theories, characteristic for so-called type-I or type-II fracton phases, respectively. Both variants have been associated with distinctive singular patterns in the spin structure factor, such as multifold pinch points for type-I and quadratic pinch points for type-II fracton phases. Here, we assess the impact of quantum fluctuations on these patterns by numerically investigating the spin S=1/2 quantum version of a classical spin model on the octahedral lattice featuring exact realizations of multifold and quadratic pinch points, as well as an unusual pinch line singularity. Based on large scale pseudofermion and pseudo-Majorana functional renormalization group calculations, we take the intactness of these spectroscopic signatures as a measure for the stability of the corresponding fracton phases. We find that in all three cases, quantum fluctuations significantly modify the shape of pinch points or lines by smearing them out and shifting signal away from the singularities in contrast to effects of pure thermal fluctuations. This indicates possible fragility of these phases and allows us to identify characteristic fingerprints of their remnants.

4-Adamantyl-(2-(arylidene)hydrazinyl)thiazoles as potential antidiabetic agents: experimental and docking studies.

Aim: To develop an efficient and cost-effective antidiabetic agent. Methods: A simple and convenient Hantzsch synthetic strategy was used to prepare 4-adamantyl-(2-(arylidene)hydrazinyl)thiazoles. Results: Fifteen newly established structures of 4-adamantyl-(2-(arylidene)hydrazinyl)thiazoles were tested for their α-amylase, antiglycation and antioxidant activities. Almost all tested compounds showed excellent α-amylase inhibition. Compounds 3a and 3j exhibited the highest potency, with IC50 values of 16.34 ± 2.67 and 16.64 ± 1.12 µM, respectively. Compounds 3c and 3i exhibited comparable antiglycation potential with the standard, aminoguanidine. The antioxidant potential of compound 3g was found to be excellent, with an IC50 value of 28.19 ± 0.2563 µM. The binding interactions of compound 3a (binding energy = -8.833 kcal/mol) with human pancreatic α-amylase identified 3a as a potent α-amylase inhibitor. Conclusion: Enrichment of established structures with more electron-donating functionalities may assist/lead to the development of more potent antidiabetic drugs.

Diabetes is one of the major causes of death in the present era. To combat damaging processes associated with diabetes, called glycation and oxidation, we prepared a series of compounds called 4-adamantyl-(2-(arylidene)hydrazinyl)thiazoles. The established structures were tested for their antidiabetic potential. The compounds 4-adamantyl-(2-(4-chlorobenzylidene)hydrazinyl)thiazole and 4-adamantyl-(2-(2-chlorobenzylidene)hydrazinyl)thiazole showed the highest potency. The compounds 4-adamantyl-(2-(4-bromobenzylidene)hydrazinyl)thiazole and 4-adamantyl-(2-(2-hydroxybenzylidene)hydrazinyl)thiazole exhibited comparable antiglycation potential. The antioxidant potential of compound 4-adamantyl-(2-(3-nitrobenzylidene)hydrazinyl)thiazole was found to be excellent. A further test was used to check toxicity and all compounds were found to be biocompatible. We also investigated, through docking studies, the way in which these compounds interact with the human proteins albumin and pancreatic α-amylase.

Coupled Effect of Polypropylene Fibers and Slag on the Impact Resistance and Mechanical Properties of Concrete.

The disposal of steel slag leads to the occupation of large land areas, along with many environmental consequences, due to the release of poisonous substances into the water and soil. The use of steel slag in concrete as a sand-replacement material can assist in reducing its impacts on the environment and can be an alternative source of fine aggregates. This is the very first paper that seeks to experimentally investigate the cumulative effect of steel slag and polypropylene fibers, particularly on the impact resistance of concrete. Various concrete mixes were devised by substituting natural sand with steel slag at volumetric replacement ratios of 0%, 10%, 20%, 30%, and 40%, with and without fibers. Polypropylene fibers of 12 mm length were introduced into the steel slag concrete at 0%, 0.5%, and 1.0% by weight of cement as a reinforcing material. Performance evaluation of each mix through extensive experimental testing indicated that the use of steel slag as partial substitution of natural sand, up to a certain optimum replacement level of 30%, considerably improved the compressive strength, flexural strength, and tensile strength of the concrete by 20.4%, 23.8%, and 17.0%, respectively. Furthermore, the addition of polypropylene fibers to the steel slag concrete played a beneficial role in the improvement of strength characteristics, particularly the flexural strength and final drop weight impact energy, which had a maximum rise of 48.1% and 164%, correspondingly. Moreover, integral structure and analytical analyses have also been performed in this study to validate the experimental findings. The results obtained encourage the use of fiber-reinforced steel slag concrete (FRSLC) as a potential impact-resistant material considering the environmental advantages, with the suggested substitution, of an addition ratio of 30% and 1.0% for steel slag and polypropylene fibers, respectively.

A Novel Homomorphic Approach for Preserving Privacy of Patient Data in Telemedicine.

Globally, the surge in disease and urgency in maintaining social distancing has reawakened the use of telemedicine/telehealth. Amid the global health crisis, the world adopted the culture of online consultancy. Thus, there is a need to revamp the conventional model of the telemedicine system as per the current challenges and requirements. Security and privacy of data are main aspects to be considered in this era. Data-driven organizations also require compliance with regulatory bodies, such as HIPAA, PHI, and GDPR. These regulatory compliance bodies must ensure user data privacy by implementing necessary security measures. Patients and doctors are now connected to the cloud to access medical records, e.g., voice recordings of clinical sessions. Voice data reside in the cloud and can be compromised. While searching voice data, a patient's critical data can be leaked, exposed to cloud service providers, and spoofed by hackers. Secure, searchable encryption is a requirement for telemedicine systems for secure voice and phoneme searching. This research proposes the secure searching of phonemes from audio recordings using fully homomorphic encryption over the cloud. It utilizes IBM's homomorphic encryption library (HElib) and achieves indistinguishability. Testing and implementation were done on audio datasets of different sizes while varying the security parameters. The analysis includes a thorough security analysis along with leakage profiling. The proposed scheme achieved higher levels of security and privacy, especially when the security parameters increased. However, in use cases where higher levels of security were not desirous, one may rely on a reduction in the security parameters.

Extraction and characterization of polyphenols from non-conventional edible plants and their antioxidant activities.

Narrow leaf plantain, white clover, perennial ryegrass, and tall fescue are non-conventional edible plants having widespread existence in many temperate and Mediterranean regions worldwide. These edible plants represent valuable food resources, and genetic and genomic approaches to improving and utilising these plants for human consumption are ongoing. These plants have characteristic high antioxidant capacities attributed to their polyphenol composition. This study performed the screening of polyphenols by applying a high-throughput LC-ESI-QTOF/MS technique and determined their antioxidant potential. Seventy different polyphenols were detected with 25 compounds in narrow leaf plantain, 27 in white clover, 15 in perennial ryegrass and 14 in tall fescue. Total phenolic content (TPC) was highest in white clover (1.81 ± 0.16 mg GAE/g fresh weight (FW)), while total tannin content (TTC) was highest in perennial ryegrass (0.89 ± 0.04 mg GAE/g FW) compared with their three counterparts, respectively. Narrow leaf plantain and white clover had greater total flavonoid content (TFC) than perennial ryegrass and tall fescue. The results of this investigation provided valuable information about the unique phenolic composition and antioxidant potential of the studied non-conventional edible plants that could be used to promote their utilization in human foods, nutraceutical preparations and functional foods besides being used as a valuable source of polyphenols for different industrial sectors. Besides, the results of the study can also be used as a baseline information for the planned and targeted delivery of bioactive compounds like polyphenols to the animals by devising an appropriate feeding strategy based on the phenolic composition of these plants.

Investigation of genetic relationships within three Miscanthus species using SNP markers identified with SLAF-seq.

BACKGROUND: Miscanthus, which is a leading dedicated-energy grass in Europe and in parts of Asia, is expected to play a key role in the development of the future bioeconomy. However, due to its complex genetic background, it is difficult to investigate phylogenetic relationships in this genus. Here, we investigated 50 Miscanthus germplasms: 1 female parent (M. lutarioriparius), 30 candidate male parents (M. lutarioriparius, M. sinensis, and M. sacchariflorus), and 19 offspring. We used high-throughput Specific-Locus Amplified Fragment sequencing (SLAF-seq) to identify informative single nucleotide polymorphisms (SNPs) in all germplasms. RESULTS: We identified 257,889 SLAF tags, of which 87,162 were polymorphic. Each tag was 264-364 bp long. The obtained 724,773 population SNPs were used to investigate genetic relationships within three species of Miscanthus. We constructed a phylogenetic tree of the 50 germplasms using the obtained SNPs and grouped them into two clades: one clade comprised of M. sinensis alone and the other one included the offspring, M. lutarioriparius, and M. sacchariflorus. Genetic cluster analysis had revealed that M. lutarioriparius germplasm C3 was the most likely male parent of the offspring. CONCLUSIONS: As a high-throughput sequencing method, SLAF-seq can be used to identify informative SNPs in Miscanthus germplasms and to rapidly characterize genetic relationships within this genus. Our results will support the development of breeding programs with the focus on utilizing Miscanthus cultivars with elite biomass- or fiber-production potential for the developing bioeconomy.

Nature of Unconventional Pairing in the Kagome Superconductors AV_{3}Sb_{5} (A=K,Rb,Cs).

The recent discovery of AV_{3}Sb_{5} (A=K,Rb,Cs) has uncovered an intriguing arena for exotic Fermi surface instabilities in a kagome metal. Among them, superconductivity is found in the vicinity of multiple van Hove singularities, exhibiting indications of unconventional pairing. We show that the sublattice interference mechanism is central to understanding the formation of superconductivity in a kagome metal. Starting from an appropriately chosen minimal tight-binding model with multiple van Hove singularities close to the Fermi level for AV_{3}Sb_{5}, we provide a random phase approximation analysis of superconducting instabilities. Nonlocal Coulomb repulsion, the sublattice profile of the van Hove bands, and the interaction strength turn out to be the crucial parameters to determine the preferred pairing symmetry. Implications for potentially topological surface states are discussed, along with a proposal for additional measurements to pin down the nature of superconductivity in AV_{3}Sb_{5}.

Magnetic Field Induced Quantum Spin Liquid in the Two Coupled Trillium Lattices of K_{2}Ni_{2}(SO_{4})_{3}.

Quantum spin liquids are exotic states of matter that form when strongly frustrated magnetic interactions induce a highly entangled quantum paramagnet far below the energy scale of the magnetic interactions. Three-dimensional cases are especially challenging due to the significant reduction of the influence of quantum fluctuations. Here, we report the magnetic characterization of K_{2}Ni_{2}(SO_{4})_{3} forming a three-dimensional network of Ni^{2+} spins. Using density functional theory calculations, we show that this network consists of two interconnected spin-1 trillium lattices. In the absence of a magnetic field, magnetization, specific heat, neutron scattering, and muon spin relaxation experiments demonstrate a highly correlated and dynamic state, coexisting with a peculiar, very small static component exhibiting a strongly renormalized moment. A magnetic field B≳4 T diminishes the ordered component and drives the system into a pure quantum spin liquid state. This shows that a system of interconnected S=1 trillium lattices exhibits a significantly elevated level of geometrical frustration.

Nanoparticles encapsulation of Phoenix dactylifera (date palm) mucilage for colonic drug delivery.

Compressed tablet formation from granular drug require binder with Cohesiveness property. Plants mucilage as pharmaceutical excipients are available. In this study, biocompatible date palm mucilage was encapsulated with silver nano particles for sustained drug release to provoke an immune response. Nano formulated mucilage was characterized by UV/VIS, FTIR, XRD, SEM/EDX spectrophotometry. UV/VIS spectra revealed an intense surface plasmon resonance peak at 406 nm for spherical mono dispersed silver nano formulated mucilage resulted from efficient reduction of silver ions to AgNPs. Zeta sizer disclosed the emergence of single peak at 139.7 nm with 100% intensity. Crude mucilage exhibited number of peaks in the region of 4000-500 cm-1 by FT-IR spectroscopy whereas purified as well as nano formulated samples showed somewhat different pattern of peaks in addition to peaks of crude sample. XRD spectra of crude mucilage revealed somewhat regular pattern while purified and modified mucilage displayed irregular structure. In SEM analysis, crude mucilage was appeared as granular that turned into porous network with entangled tiny silver nano spheres. A controlled release of drug levofloxacin hemihydrate was evaluated using crude/ nano formulated mucilage as excipient. Nano formulated mucilage delayed the onset exposure of drug in gastric medium giving recommendations as value added bio binder for drug to the target organ.

Green Synthesis of CeO2 Nanoparticles from the Abelmoschus esculentus Extract: Evaluation of Antioxidant, Anticancer, Antibacterial, and Wound-Healing Activities.

Metal oxide nanoparticles synthesized by the biological method represent the most recent research in nanotechnology. This study reports the rapid and ecofriendly approach for the synthesis of CeO2 nanoparticles mediated using the Abelmoschus esculentus extract. The medicinal plant extract acts as both a reducing and stabilizing agent. The characterization of CeO2 NPs was performed by scanning electron microscopy (SEM), X-ray diffraction (XRD), ultraviolet-visible spectroscopy (UV-Vis), and Fourier transform infrared spectroscopy (FTIR). The in vitro cytotoxicity of green synthesized CeO2 was assessed against cervical cancerous cells (HeLa). The exposure of CeO2 to HeLa cells at 10-125 µg/mL caused a loss in cellular viability against cervical cancerous cells in a dose-dependent manner. The antibacterial activity of the CeO2 was assessed against S. aureus and K. pneumonia. A significant improvement in wound-healing progression was observed when cerium oxide nanoparticles were incorporated into the chitosan hydrogel membrane as a wound dressing.

LC-ESI/QTOF-MS Profiling of Chicory and Lucerne Polyphenols and Their Antioxidant Activities.

Chicory and lucerne are used as specialised forages in sheep or dairy production systems in some parts of the world. Recently, these plants are gaining attention as raw materials in the search for natural antioxidants for use in animal feeds, human foods and nutraceutical formulations. The antioxidant potential of these plants is credited to polyphenols, a subgroup of phytochemicals. Therefore, phenolic characterisation is an essential step before their use as ingredients in animal feeds, human food or nutraceutical preparations. In this study, we performed qualitative and quantitative analysis of polyphenols in chicory and lucerne. Profiling of polyphenols from chicory and lucerne was performed by LC-ESI/QTOF-MS with a total of 80 phenolic compounds identified in chicory and lucerne. The quantification of polyphenols was achieved by high performance liquid chromatography, coupled with a photo diode array (HPLC-PDA). Chicoric acid was the major phenolic acid found in chicory, with the highest concentration (1692.33 ± 0.04 µg/g DW) among all the polyphenols quantified in this study. 2-hydroxybenzoic acid was the major phenolic acid found in lucerne, with the highest concentration of 1440.64 ± 0.04 µg/g DW. Total phenolic, flavonoids and total tannin contents were measured, and the antioxidant potential was determined by 2,2-Diphenyl-1-picrylhydrazyl, Ferric Reducing Antioxidant Power, 2,2-Azino-bis-3-ethylbenzothiazoline-6-sulfonic Acid, Hydroxyl (OH-) Radical Scavenging Activity, Chelating Ability of Ferrous Ion (Fe2+) and Reducing Power (RPA) assays. Both chicory (8.04 ± 0.33 mg AAE/g DW) and lucerne (11.29 ± 0.25 mg AAE/g DW) showed high values for Hydroxyl (OH-) Radical Scavenging Activity. The current study allowed us to draw a profile of polyphenols from chicory and lucerne. They provided a molecular fingerprint useful for the application of these plant materials in human foods, animal feeds and pharmaceutical formulations.

Seasonal Nutrient Cycling and Enrichment of Nutrient-Related Soil Microbes Aid in the Adaptation of Ramie (Boehmeria nivea L.) to Nutrient-Deficient Conditions.

The breeding for varieties tolerant of adverse growing conditions is critical for sustainable agriculture, especially for ramie (Boehmeria nivea L.). However, a lack of information on the tolerance of ramie to nutrient-deficient conditions has hindered efforts to breed ramie varieties tolerant of such conditions. The main objective of this study was to explore the tolerance strategies of ramie plants under poor soil conditions using long-term (8-9 years) field trials. Genotypes of Duobeiti 1 and Xiangzhu XB were highly tolerant of poor soil conditions. The contributions of seasonal nutrient cycling and rhizobacteria to the ability of ramie to tolerate poor soil were tested. Nitrogen and phosphorus retranslocation to the root at the end of the growing season helped ramie adapt to poor soil conditions. The contribution of the microbial community was analyzed using high-throughput Illumina MiSeq sequencing technology. The enrichment of beneficial bacteria (mainly Bradyrhizobium, Gaiella, and norank_o_Gaiellales) and the reduction of harmful fungi (mainly Cladosporium and Aspergillus) also contributed to the ability of ramie to tolerate poor soils. The results of this study provide new insight into the ability of ramie to tolerate adverse conditions and aid future efforts to breed and cultivate ramie tolerant of adverse conditions.

Gut Microbiota-Polyphenol Interactions in Chicken: A Review.

The gastrointestinal tract of the chicken harbors very complex and diverse microbial communities including both beneficial and harmful bacteria. However, a dynamic balance is generally maintained in such a way that beneficial bacteria predominate over harmful ones. Environmental factors can negatively affect this balance, resulting in harmful effects on the gut, declining health, and productivity. This means modulating changes in the chicken gut microbiota is an effective strategy to improve gut health and productivity. One strategy is using modified diets to favor the growth of beneficial bacteria and a key candidate are polyphenols, which have strong antioxidant potential and established health benefits. The gut microbiota-polyphenol interactions are of vital importance in their effects on the gut microbiota modulation because it affects not only the composition of gut bacteria but also improves bioavailability of polyphenols through generation of more bioactive metabolites enhancing their health effects on morphology and composition of the gut microbiota. The object of this review is to improve the understanding of polyphenol interactions with the gut microbiota and highlights their potential role in modulation of the gut microbiota of chicken.

Evidence for a three-dimensional quantum spin liquid in PbCuTe2O6.

The quantum spin liquid is a highly entangled magnetic state characterized by the absence of static magnetism in its ground state. Instead, the spins fluctuate in a highly correlated way down to the lowest temperatures. Quantum spin liquids are very rare and are confined to a few specific cases where the interactions between the magnetic ions cannot be simultaneously satisfied (known as frustration). Lattices with magnetic ions in triangular or tetrahedral arrangements, which interact via isotropic antiferromagnetic interactions, can generate such a frustration. Three-dimensional isotropic spin liquids have mostly been sought in materials where the magnetic ions form pyrochlore or hyperkagome lattices. Here we present a three-dimensional lattice called the hyper-hyperkagome that enables spin liquid behaviour and manifests in the compound PbCuTe2O6. Using a combination of experiment and theory, we show that this system exhibits signs of being a quantum spin liquid with no detectable static magnetism together with the presence of diffuse continua in the magnetic spectrum suggestive of fractional spinon excitations.

Recurrence Rate of Giant Cell Tumor With the Treatment of Scooping Curettage, Burr Down Technique, Phenolization, and Bone Cement.

OBJECTIVE: To find the recurrence and outcomes of giant cell tumors treated with scooping curettage, burr down technique, phenolization, and bone cement. METHOD: We conducted a descriptive case series using a non-probability consecutive sampling technique at the Department of Orthopedics, Lahore General Hospital, Lahore, Pakistan, from May 2014 to June 2018. A total of 40 patients aged between 20 to 40 years with Compannacci grade I, II & III giant cell tumors (GCT) were included and patients unfit for the surgery, those with multiple, recurrent, malignant giant cell tumors, tumors involving the axial skeleton, and previously treated cases were excluded. We recorded the side, site of the tumor, post-operative distal neurovascular status, and recurrence of giant cell tumors. The patients were follow-up in the out-patient department (OPD) at the second week, fourth week, 12th week, 24th week, 48th week, 96th week, and 144th week after the surgery. Side, site of the tumor, and post-operative distal neurovascular status were assessed clinically, and recurrence of the tumors was observed clinically and radiologically. RESULTS: The mean age of all patients was 25.75±5.74 years. Males were 45% (18) and females were 55% (22). Most (12, 30%) tumors were present in the upper limb, and 22 (70%) were present in the lower limb. The majority (24, 60%) tumors were present around the knee joint. Companacci grade I was five (12.5%), grade II was 14 (35%), and grade III was 21 (52%). There were six (15%) pathological fractures. There was no case of distal neurovascular (DNV) injury, and three patients had a recurrence in two years of follow-up. CONCLUSION: Giant cell tumor treated with scooping curettage, burr down technique, phenolization and poly-methyl methacrylate showed 7.5% recurrence. The combined use of local adjuvants in the treatment of giant cell tumors is a safe and effective way to reduce the rate of local recurrence.

Molecular cytogenetic characterization and phylogenetic analysis of four Miscanthus species (Poaceae).

Chromosomes of four Miscanthus (Andersson, 1855) species including M. sinensis (Andersson, 1855), M. floridulus (Schumann & Lauterb, 1901), M. sacchariflorus (Hackel, 1882) and M. lutarioriparius (Chen & Renvoize, 2005) were analyzed using sequentially combined PI and DAPI (CPD) staining and fluorescence in situ hybridization (FISH) with 45S rDNA probe. To elucidate the phylogenetic relationship among the four Miscanthus species, the homology of repetitive sequences among the four species was analyzed by comparative genomic in situ hybridization (cGISH). Subsequently four Miscanthus species were clustered based on the internal transcribed spacer (ITS) of 45S rDNA. Molecular cytogenetic karyotypes of the four Miscanthus species were established for the first time using chromosome measurements, fluorochrome bands and 45S rDNA FISH signals, which will provide a cytogenetic tool for the identification of these four species. All the four have the karyotype formula of Miscanthus species, which is 2n = 2x = 38 = 34m(2SAT) + 4sm, and one pair of 45S rDNA sites. The latter were shown as strong red bands by CPD staining. A non-rDNA CPD band emerged in M. floridulus and some blue DAPI bands appeared in M. sinensis and M. floridulus. The hybridization signals of M. floridulus genomic DNA to the chromosomes of M. sinensis and M. lutarioriparius genomic DNA to the chromosomes of M. sacchariflorus were stronger and more evenly distributed than other combinations. Molecular phylogenetic trees showed that M. sinensis and M. floridulus were closest relatives, and M. sacchariflorus and M. lutarioriparius were also closely related. These findings were consistent with the phylogenetic relationships inferred from the cGISH patterns.

Environmentally responsive and anti-bugs textile finishes - Recent trends, challenges, and future perspectives.

Bugs, such as microorganisms and insects, are present in the environment and sometimes can be health-hazardous if the living environment is not maintained following proper hygienic regulations. In the present scenario of increasing public awareness, environmental consciousness, and growing demand for easy-care, and disinfected textiles, the manufacturing of protective and easy-to-care textiles has become a key necessity of the modern world. Comfortable, clean, hygienic, antimicrobial, and insect repelling properties of textile goods are gaining the accelerating research momentum as a basic requirement to produce multifunctional textiles. These functional finishes have numerous applications such as in-home textiles, bed nets, and tenting, camping gear as well as in military uniforms. Synthetic antimicrobial and insect repellents are quite effective against insects and microscopic organisms but are slightly toxic to the human being and the environment. To overcome these problems, researchers are considering natural agents for functional finishes, but their effectiveness is less durable to textile material. Besides needful advantages, the excessive use of dyes in finishing processes heavily required washing cycles and ultimately release various types of hazardous dyes or wasteful effluents in the environment. This review reports the chemical composition and recent developments in textile finishes, particularly antimicrobial and insect repellent textile finishes. A large number of commonly used antimicrobial agents (i.e. chitosan, zwitterionic compounds, silver and silver-based compounds, titanium dioxide nanoparticles, imidazolium salts, triclosan and quaternary ammonium salts) and insect repellent textile finishes (i.e. N­N­diethyl­m­toluamide, permethrin, cypermethrin, pyrethrum, picaridin, bioallethrin, citriodiol and essential oils) have been presented. Finally, the review is wrapped up with major research gaps/challenges, concluding remarks, and future opportunities in this area of research.