The differences in the number of fibroblasts and blood vessels after the topical and systemic administration of Lactobacillus casei Shirota probiotics for the treatment of traumatic ulcers in Wistar rats (Rattus norvegicus).

BACKGROUND AND AIM: The use of drugs as a therapy for traumatic ulcers may lead to drug resistance and other side effects. Lactobacillus casei Shirota can affect the number of fibroblasts and blood vessels in wound healing. The aim of this study was to investigate the difference in the number of fibroblast cells and blood vessels after the topical and systemic administration of L. casei Shirota probiotics in Wistar rats with traumatic ulcer. MATERIALS AND METHODS: Overall, 36 healthy male Wistar rats aged 2-3 months old and weighing 175-250 g in body weight were used as a sample. Traumatic ulcer was made on the labial fornix incisive inferior. The subject rats were divided into groups: (1) A control group over 3 days, (2) a group that used distilled water over 7 days, (3) a group that underwent topical treatment over 3 days, (4) a group that used probiotics administered topically over 7 days, (5) a group that underwent systemic treatment over 3 days, and (6) a group that took oral probiotics for the traumatic ulcers over 7 days. The number of fibroblasts and blood vessels was observed through a hematoxylin-eosin examination. RESULTS: Based on the results of the study, a significant difference was observed in the number of fibroblasts (p=0.00) and blood vessels (p=0.018) in the 3-day topical group that underwent a 3-day systemic administration of probiotics compared with the number of fibroblast cells in the 7-day topical group and 7-day systemic group (p=0.00). CONCLUSION: Overall, significant differences were observed in the number of fibroblasts and blood vessels in Wistar rats with traumatic ulcer after undergoing the topical and systemic administration of L. casei Shirota probiotics.

The Risk of Night Shift Workers to the Glucose Blood Levels, Saliva, and Dental Caries.

OBJECTIVE: This study aimed to provide the correlation of circadian rhythms of night shift workers with blood glucose levels, saliva, and dental caries. MATERIALS AND METHODS: This study was conducted on night shift and nonshift workers to measure fasting blood glucose (FBG), 2-hour postprandial blood glucose (2-PP BG), saliva flow rate, pH saliva, and caries with the decay-missing-filled total (DMF-T) index. Data analysis was done using independent t-test and correlation test with Pearson correlation. RESULTS: There were significant differences in night shift and nonshift workers in FBG (p = 0.000), 2-PP BG (p = 0.000), flow rate saliva (p = 0.000), and DMF-T index (p = 0.001). Correlation test showed positive correlation between FBG and pH saliva (r = 0.42, p = 0.029) and DMF-T index (r = 0.521, p = 0.005) of night shift workers. The 2-PP BG also showed positive correlation with pH saliva (r = 0.493, p = 0.009) and DMF-T index (r = 0.743, p = 0.000). The DMF-T index showed negative correlation with flow rate saliva (r = -0.398, p = 0.04). In the nonshift workers, correlation test showed a correlation between FBG and DMF-T index (r = 0.384, p = 0.048). The DMF-T index showed correlation with flow rate saliva (r = 0.6, p = 0.001). CONCLUSION: There is a circadian rhythm correlation between night shift workers to blood glucose levels, flow rate saliva, pH saliva, and dental caries.

The effect of moderate exercise on the elevation of Bax/Bcl-2 ratio in oral squamous epithelial cells induced by benzopyrene.

AIM: The aim of this study was to analyze the effect of moderate exercise on the elevation of Bax/Bcl-2 ratio. MATERIALS AND METHODS: Eighteen Mus musculus strain Swiss Webster (Balb/c) were divided into three groups (n=6). K1 and K2 had contact with water 3 times/week for 12 weeks, while the members of the K3 group swam 3 times/week for 12 weeks while carrying load weighed 3% of their body weight. After 5 weeks, they were induced with 0.04 ml oleum olivarum (K1), 0.08 mg benzopyrene/0.04 ml oleum olivarum (K2, K3) 3 times/week for 4 weeks. Immunohistochemistry assays were used to determine the ratio of Bax/Bcl-2 expression. The results were analyzed using an independent t-test. RESULT: The Bax/Bcl-2 ratio increased significantly in K3 compared to K2 (p=0.00). CONCLUSION: Moderate exercise could increase the Bax/Bcl-2 ratio in oral squamous epithelial cells induced by benzopyrene.