Laboratory studies of the diffusive transport of 137Cs and 60Co through potential waste repository soils.

Tests using reconstituted samples have been performed to assess the diffusive transport of (137)Cs and (60)Co through natural regolith materials from a region in South Australia being considered for a radioactive waste repository. A double diffusion cell apparatus made of polycarbonate resin was developed to estimate the effective diffusion (D(e)) and sorption coefficients (K(d)) that allowed large withdrawals from the source and collector cells and has enabled tests with low concentrations of radioactivity. An alternative to porous stainless steel filter plates has also been used to reduce uncertainty in test interpretation. Analysis of the transient data used a staged method of the Laplace transform to take into consideration the volume of the samples withdrawn from the apparatus during testing. At test completion samples were cut into slices and analysed for radionuclide concentration. Data obtained from the sliced samples confirmed that both numerical and experimental data produced acceptable mass balance. The D(e) values obtained in this study were of the order of 10(-6) cm(2) s(-1) for both species, higher than previously published data. The K(d) values from the diffusion and batch sorption tests were in reasonable agreement for (137)Cs, but an order of magnitude different for (60)Co. The sorption of the latter radionuclide was strongly pH dependent, and this dependency during diffusion tests would benefit from further investigation.

High-level expression and bulk crystallization of recombinant L-methionine gamma-lyase, an anticancer agent.

L-Methionine gamma-lyase is a pyridoxal 5'-phosphate-dependent enzyme which has tumor selective anticancer activity. An efficient production process for the recombinant enzyme was constructed by using the overexpression plasmid in Escherichia coli, large-scale cultivation, and practical crystallization on an industrial scale. The plasmid was optimized with a promoter and the region of the ribosome-binding site. Plasmid pMGLTrc03, which has a trc promoter and a spacing of 12 nucleotides between the Shine-Dalgarno sequence and the ATG translation initiation codon, was selected as the most suitable plasmid. The transformants produced the enzyme, which intracellularly accumulated at 2.1 mg/ml as an active form and accounted for 43% of the total proteins in the soluble fraction by simple batch fermentation using a 500-l fermentor. The crystals were directly obtained from crude enzyme with 87% yield by a crystallization in the presence of 9.0% polyethylene glycol 6000, 3.6% ammonium sulfate, and 0.18 M sodium chloride using a 100-l crystallizer. After recrystallization, the enzyme was purified by anion-exchange column chromatography to remove endotoxins and by gel filtration for polishing. We prepared 600 g of purified enzyme with a low endotoxin content of sufficient quality for therapeutical use, with a 41% overall yield in the purification process.

Properties of two tropical soils in relation to the transport of radionuclides in the rhizosphere.

The uptake of radionuclides by commercial crops is being studied at two sites, Blain and Tippera, in a research farm in the Northern Territory, Australia. Studies have been performed to characterise the properties of the two soils, particularly the hydraulic properties that are considered to significantly influence the transport and plant uptake of these radionuclides in the soils The Blain soil, a sandy loam, has been categorised as SM according to the Unified Soil Classification System. Quartz is the dominant mineral for the Blain soil. The Tippera soil, a kaolinitic clayey loam has been categorised as CL. Chemical analysis results were consistent with these findings. The saturated hydraulic conductivity values were of the order of 10(-4) cm/s for the Blain soil. These values were greater by 3-4 orders of magnitude than those for the Tippera soils. The results obtained from the hydraulic property measurements were used to estimate the unsaturated hydraulic properties. A bimodal description based on van Genuchten-type partial saturation functions was used for the estimation. The estimation was qualitatively consistent with the soil types.