[Regulation of the adenylate cyclase system of lymphocytes in patients with therapy-resistant arterial hypertension and during PGE2 administration]. / Reguliatsiia adenilattsiklaznoi sistemy limfotsitov u bol'nykh arterial'noi gipertoniei, refrakternoi k terapii, pri vvedenii pge2.

The effects of prostaglandin E2 (PGE2) infusions on lymphocyte beta 2-adrenoceptor-dependent adenylate cyclase (beta 2ARAC) system were studied in 26 patients with resistant hypertension (RH). The density of beta 2-adrenoceptors and their affinity for 1-isoproterenol were measured with 125ICYP and adenylate cyclase activity was determined by alpha-[32P]AMP generation in mononuclear lymphocytes of RH patients before and 1, 7, and 14 days after the last PGE2 infusion. Plasma epinephrine and norepinephrine concentrations were analyzed by using HPLC. The resistance to the four-component drug therapy was overcome with PGE2 infusions in patients, leading to a decrease in lymphocyte beta 2-adrenoceptor density and an increase in beta 2-receptor affinity for catecholamines and in adenylate cyclase activation by stimulating agents. PGE2 infusions unchanged the sensitivity to antihypertensive drugs in patients resulting in an increase in lymphocyte beta 2-adrenoceptor density, a decrease in beta 2-receptor affinity for catecholamines and in adenylate cyclase activation.

[Determination of free catecholamines in the urine using high efficiency liquid chromatography with an electrochemical detector]. / Opredelenie svobodnykh katekholaminov mochi metodom vysokoéffektivnoi zhidkostnoi khromatografii s élektrokhimicheskim detektorom.

The authors describe electrochemical detector high-pressure liquid chromatography (EDHPLC) technique for measuring nanogram amounts of catecholamines in human urine. Alumina-treated samples are layered onto a reverse-phase column for the separation of catecholamines, with a monochloracetate buffer/acetonitrile mixture containing 75 mg of sodium octyl sulfate as the mobile phase. Electrochemical detector was employed for the quantitative detection of catecholamines. Catecholamine levels of up to 1000 ng are detectable with the use of electrochemical detector. EDHPLC is a highly suitable method for clinical investigations.