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1.
Bioorg Khim ; 38(4): 431-8, 2012.
Artigo em Russo | MEDLINE | ID: mdl-23189557

RESUMO

Ability of site-specific nickase BspD6I (Nt.BspD6I) to oligomerize at concentrations > or = 0.5 microM (> or = 0.035 mg/mL) is studied. Three states of Nt.BspD6I are registered via electrophoretic studies both in the presence and in the absence of DNA. Estimation of their molecular mass allows assigning them as a monomer, a dimer and a trimer. Both dimeric and monomeric Nt.BspD6I are shown to hydrolyze its DNA substrate with the identical specificity. Calculation of the electrostatic potential distribution on the Nt.BspD6I globule surface shows that the protein molecule is a dipole. The Nt. BspD6I oligomeric forms are likely to be the result of ionic protein interactions.


Assuntos
Proteínas de Ligação a DNA/química , Desoxirribonuclease I/química , Estrutura Terciária de Proteína , Bacillus/enzimologia , DNA/química , Multimerização Proteica
2.
Biofizika ; 57(3): 432-6, 2012.
Artigo em Russo | MEDLINE | ID: mdl-22873066

RESUMO

Nicking endonuclease Nt.BspD6I is a heterodimeric restriction endonuclease, one subunit of which exhibits specific nicking activity. It gets bound to double-stranded DNA and makes a break (nick) in one chain at a distance of 4 nucleotides from the binding site. In this work, for visualization of the specific binding and protein landing site an atomic force microscopy was used. In five minutes after incubation of DNA solution with nicking endonuclease, the DNA molecules with associated proteins which located at the expected binding site and "shared" DNA strand into two segments (approximately, 1/3 and 2/3 of length) were observed in the images. In addition, near the binding site DNA molecule had a height corresponding to a single-stranded DNA molecule, which was in good agreement with single-stranded cleavage by nickase in the course of complex formation.


Assuntos
Bacillus/enzimologia , DNA de Cadeia Simples/química , DNA/química , Desoxirribonuclease I/química , Sítios de Ligação , DNA/ultraestrutura , DNA de Cadeia Simples/ultraestrutura , Microscopia de Força Atômica , Subunidades Proteicas/química , Especificidade por Substrato
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