RESUMO
This paper reports on morphophysiological and biochemical characteristics of control and potato plants (Solarium tuberosum L., Skoroplodnyi cultivar) transformed with the Δ12 acyl-lipid desaturase gene (desA) grown long-term in vitro. The transformed plants showed faster growth and faster ontogenesis as compared to controls, which was accompanied with changes in the accumulation of photosynthetic pigments (chlorophylls a and b, carotenoids) and phenolic compounds, including flavonoids in the leaves. These characteristics were pronounced to a high degree in Line II plants with high expression rates of the desA gene, whereas Line I plants (moderate expression rate) were similar to control plants in many parameters.
Assuntos
Ácidos Graxos Dessaturases/biossíntese , Plantas Geneticamente Modificadas/genética , Solanum tuberosum/genética , Carotenoides , Clorofila/metabolismo , Clorofila A , Ácidos Graxos Dessaturases/genética , Regulação da Expressão Gênica de Plantas , Fotossíntese/genética , Plantas Geneticamente Modificadas/crescimento & desenvolvimento , Solanum tuberosum/crescimento & desenvolvimento , Solanum tuberosum/metabolismoRESUMO
Experimental models of the potato primary transgenic plants which express the hybrid gene cry3aM-licBM2 have been created. Modecular analysis and the biotests of the experimental models allow proposing a new system of cry genes expression in plants. The system is based on the expression of hybrid genes possessing the sequence of reporter lichenase gene and the use as a regulator element of a light-induced promoter providing preferential expression of the controled genes only in green plant tissues (leaves)--the target tissues for pests. The lichanase presence in hybrid proteins facilitates selection and analysis of the expression level of the hybris proteins in transgenic organisms. Basing on the lichenase properties in hybrid proteins it seems possible to use this reporter system for transgene monitoring in agrocoenosis as this system is rather simple and precise and does not need large material and time expenses.
Assuntos
Modelos Genéticos , Plantas Geneticamente Modificadas/genética , Solanum tuberosum/genética , Animais , Toxinas de Bacillus thuringiensis , Proteínas de Bactérias/genética , Toxinas Bacterianas/genética , Besouros/fisiologia , Endotoxinas/genética , Comportamento Alimentar/fisiologia , Genes Reporter , Glicosídeo Hidrolases/genética , Proteínas Hemolisinas/genética , Controle Biológico de Vetores/métodos , Folhas de Planta/enzimologia , Folhas de Planta/genética , Folhas de Planta/crescimento & desenvolvimento , Folhas de Planta/parasitologia , Plantas Geneticamente Modificadas/enzimologia , Plantas Geneticamente Modificadas/crescimento & desenvolvimento , Plantas Geneticamente Modificadas/parasitologia , Proteínas Recombinantes de Fusão/genética , Saccharomyces cerevisiae/genética , Solanum tuberosum/enzimologia , Solanum tuberosum/crescimento & desenvolvimento , Solanum tuberosum/parasitologiaRESUMO
Transgenic popato plants have been created which express recombinant proteins, analogues of spidroin 1, the protein of the cobweb skeleton thread. Expression of the hybrid spidroin 1 genes possessing some repeated sequences retains both in the model test-tube-growing plants and in the crops. Expression level of the synthetic spidroin 1 genes and the level of accumulation of their products in plants depend on the type of promoter, number of repeats, organ specificity and plant species but not on the duration of plant material storage. The results show that the strategy based on constuction and expression of hybrid proteins which include the reporter protein makes it easier to select and analyse expression of hybrid proteins in transgenic organisms.