[Immunoenzyme method of the determination of human chorionic gonadotropin using polyclonal antibodies]. / Immunofermentnyi metod opredeleniia khorionicheskogo gonadotropina cheloveka s ispol'zovaniem poliklonal'nykh antitel.

The authors developed a method for obtaining highly specific polyclonal antibodies reacting with different sites of human chorionic gonadotropin (HCG) and an immunometrical method for measuring HCG in human biological fluids, based on the use of these antibodies. The sensitivity of the method is 10-15 IU/liter HCG, specificity 100%, no cross reactions with LH or FSH were observed. The method was tried in testing urine samples for HCG in women at 1-2 to 34 weeks of gestations and in one cancer patient with the diagnosis of vesical mole.

[Receptor properties of the hemagglutinin of influenza virus and its polypeptide fragments]. / Retseptornye svoistva gemaggliutinina virusa grippa i ego polipeptidnykh fragmentov.

The receptor properties of influenza virus A/Kiev/59/79 R (H1N1) and a number of its polypeptide fragments containing the aminoacids (from the 1st to 272d) of the heavy chain were studied. Two kinds of radioimmunoassay were used to test hemagglutinin or its polypeptide fragment interactions with cellular receptors. The studied polypeptides and hemagglutinin are shown to be capable of specific interactions with the receptors on the cell surface. The main linear fragment of hemagglutinin recognizing cellular receptors is localized within a polypeptide fragment including 1st-272d aminoacids of the heavy chain of hemagglutinin. The breaks of all the the S-S linkages including the ones linearly and spatially close to the receptor "pocket" of the bridge 95-135 do not affect significantly the receptor properties of the polypeptide.

[Mechanism of enzymatic imprinting induced in rats by an early postnatal administration of galactose]. / Izuchenie mekhanizma fermentativnogo imprintinga, indutsirovannogo u krys rannim postnatal'nym vvedeniem galaktozy.

Administration of galactose into young rats within an early postnatal period led to alteration in activity of some enzymes involved in utilization of galactose (galactose-1-phosphaturidyl transferase, galactose-6-phosphate dehydrogenase etc) for a long period of the animals life. This stable alteration in activity of adaptive enzymes was characterized as the enzymatic imprinting. After administration of galactose into neonatal animals synthesis of RNA, matrix activity of chromatin, activities of DNA-dependent RNA polymerase and RNA-dependent DNA polymerase were shown to increase in liver tissue of these animals. These alterations are considered as a possible basis for the stable alterations in the genes expression. The elevated activities of DNA-dependent RNA-polymerase and reverse transcriptase were maintained within a long period of the animals life.