Modification of carbon foam with 4-mercaptobenzoic acid functionalised gold nanoparticles for an application in a yeast-based microbial fuel cell.

Modification of carbon foam with gold nanoparticles (AuNPs) was successfully performed through a hydrothermal method. The modified AuNPs were functionalised with 4-mercaptobenzoic acid (MBA) to improve their affinity toward microorganisms. TEM and SEM characterization indicated that although polydisperse spherical nanoparticles of AuNPs with particle sizes around 17 nm were obtained, the attached nanoparticles were agglomerated to be around 0.4 to 1.5 µm in size on the carbon foam surface. The electrochemical studies using cyclic voltammetry technique affirmed that the modified carbon foam electrodes have electroactive properties against glucose. Evaluation of the electrode was performed for a microbial fuel cell using Candida fukuyamaensis yeast as the microorganisms. The polarization curves showed that functionalisation of AuNPs-modified carbon foam with MBA provides around three times higher current density (1226.93 mA m-2) and power density (330.61 mW m-2) compared to the unmodified one. This result indicated that the modification is suitable to improve yeast attachment on the electrode surface.

ß-Cyclodextrin/Fe3O4 nanocomposites for an electrochemical non-enzymatic cholesterol sensor.

A sensitive, specific, and miniaturized non-enzymatic cholesterol sensor was prepared based on the competition of inclusion complex formation between ß-cyclodextrin (BCD) and cholesterol, and between BCD and methylene blue (MB). BCD was immobilized on the surface of Fe3O4 magnetic nanoparticles instead of the electrode surface to increase the kinetic rate and enhance the sensitivity of the sensor. Furthermore, the use of magnetic nanocomposites and a screen-printed carbon electrode reduces the overall analysis time and simplifies the sample measurement procedures, making the sensor suitable for point-of-care analysis. The electrochemical measurement results of MB, released from the reactions between BCD and solutions containing various concentrations of cholesterol were used as the input signal to calculate the cholesterol concentrations. A good linearity as well as an excellent accuracy and repeatability in the concentration range of 0-150 µM with an estimated limit of detection of 2.88 µM could be achieved by using the amperometric technique at a constant potential of -0.43 V. The sensor showed a good selectivity in the presence of 1 mM concentrations of interfering agents, including NaCl, CaCl2, glycine, glucose, and ascorbic acid. Furthermore, a validation performed for cholesterol determination in milk samples was in agreement with the measurements performed by using the HPLC method, suggesting that the developed sensor is reliable.

Production of a polyclonal antibody against acrylamide for immunochromatographic detection of acrylamide using strip tests.

OBJECTIVE: To produce, purify, and characterize a polyclonal antibody against acrylamide (anti-AA) for an application to immunochromatographic strip tests for AA. MATERIALS AND METHODS: Polyclonal anti-AA was prepared by injecting N-acryloxysuccinimideconjugated bovine serum albumin hapten-antigen into New Zealand white rabbits. The antibody was purified using protein A, characterized using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and conjugated with gold nanoparticles (AuNP). The conjugated antibody was then characterized using UV-Vis and FTIR spectroscopy and transmission electron microscopy (TEM). Immunochromatographic strip tests were performed using sample pads, conjugated pads, test zones, control zones, and absorbent pads. Strip tests were finally validated using standard AA solutions followed by the application of various concentrations of coffee samples. RESULTS: Using SDS-PAGE, the purified anti-AA antibody was resolved at 50 and 25 kDa, indicating the presence of heavy and light chains, respectively. The conjugation of anti-AA with AuNP was confirmed using wavelength shifts in UV-Vis and FTIR spectra, and TEM analyses revealed increased diameters of AuNPs after conjugation. The immunochromatographic strip test was sensitive to 1 mgml-1 standard AA. Various concentrations of coffee samples resulted in red color differences in the test zone. High and low coffee concentrations produced thick and thin red lines, respectively. CONCLUSION: Purified anti-AA can be conjugated with AuNP to produce strip tests for detecting AA in coffee samples. The present immunochromatographic strip tests quantitatively showed increasing intensities of red lines with increasing AA concentrations.