RESUMO
The distribution of fucose-containing glycoconjugates in the photoreceptor cell layer of rat and human retinas was examined by lectin histochemistry using Aleuria aurantia lectin (AAL), which recognizes L-fucose alpha 1, 6 residue. In the rate retina, AAL diffusely bound to the apical outer segments and to the basal inner segments, whereas it bound to the entire outer segments of other photoreceptors, which were considered to be cones due to their proportion. In the human retina, AAL bound diffusely to the basal inner segments and to the retinal pigment epithelia, but it bound selectively to the outer segments of the cones. The present findings revealed that the glycoconjugates, whose sugar chains contain L-fucose alpha 1, 6 residue on their termini, are present in the cone outer segments.
Assuntos
Fucose/análise , Lectinas/metabolismo , Segmento Externo da Célula Bastonete/metabolismo , Animais , Histocitoquímica , Humanos , Técnicas In Vitro , Epitélio Pigmentado Ocular/metabolismo , Ratos , Ratos Wistar , Retina/metabolismoRESUMO
The glycoconjugates in neonate rat eyelids at postnatal day 0 or 1 were examined by lectin histochemistry. Maackia amurensis lectin II, which recognizes sialic acid alpha 2, 3 galactose beta 1, 3 N-acetylgalactosamine (Gal beta 1, 3 GalNAc) or sialic acid alpha 2, 3 galactose beta 1, 4 N-acetylglucosamine, bound to the cell membranes of the epithelial basal cells, suggesting that the glycoconjugates containing these sugar chains are present on their cell membranes. With respect to the binding of the Gal beta 1, 3 GalNAc-specific lectin, jacalin, whose binding is not inhibited by the terminal sialic acid, bound to the cell membranes of the epithelial basal cells, whereas peanut agglutinin, whose binding is inhibited by the terminal sialyl residue, did not bind to their cell membranes. These findings suggest that all the residues of Gal beta 1, 3 GalNAc in the glycoconjugates of their cell membranes are sialylated as the mature form.
Assuntos
Pálpebras/metabolismo , Glicoconjugados/metabolismo , Ácido N-Acetilneuramínico/metabolismo , Animais , Animais Recém-Nascidos , Epitélio/metabolismo , Histocitoquímica , Lectinas , Ratos , Ratos WistarAssuntos
Mucina-1/análise , Retina/química , Biomarcadores Tumorais/análise , Neoplasias da Coroide/química , Neoplasias da Coroide/patologia , Neoplasias Oculares/química , Neoplasias Oculares/patologia , Humanos , Técnicas Imunoenzimáticas , Melanoma/química , Melanoma/patologia , Células Fotorreceptoras/química , Células Fotorreceptoras/patologia , Retina/patologia , Retinoblastoma/química , Retinoblastoma/patologiaRESUMO
The glycoconjugates in eyelids of adult rats were examined by lectin histochemistry and in situ hybridization histochemistry. Since Maackia amurensis lectin II and jacalin bound to the cell membranes of basal and apical epithelial cells, sialic acid alpha 2,3 galactose (Gal) beta 1,3 N-acetylgalactosamine (GalNAc) sequence is present in the glycoconjugates of their cell membranes. Peanut agglutinin bound to the cell membranes of spinous cells in the middle of the epithelium, suggesting that Gal beta 1, 3 GalNAc sequence is present in their glycoconjugates. The mRNA of Gal beta 1,3 GalNAc alpha 2,3-sialyltransferase was detected in the cytoplasm of the epithelial cells other than the basal cells. This observation suggests that sialoglycoconjugates may be newly synthesized in the spinous and apical cells, while the glycoconjugates in the cell membranes of basal cells may be produced at an early stage of development and are stable without turnover.
Assuntos
Pálpebras/química , Glicoconjugados/análise , Animais , Células Epiteliais , Epitélio/química , Pálpebras/citologia , Histocitoquímica/métodos , Lectinas/análise , RNA Mensageiro/análise , Ratos , Ratos Wistar , Sialiltransferases/genéticaRESUMO
The distribution of O- and N-linked glycoconjugates in human conjunctival goblet cells was examined histochemically using biotinylated and fluorescence-labeled lectins simultaneously. Both peanut agglutinin and Erythrina cristagalli agglutinin, specific for O- and N-linked sugar chains, respectively, bound to the same goblet cell, which demonstrated that a conjunctival goblet cell produces and contains both types of glycoconjugates. Maackia amurensis lectin II, specific for sialic acid alpha 2, 3 galactose, bound to the goblet cells, while Sambueus nigra agglutinin, specific for sialic acid alpha 2, 6 galactose, did not. This observation suggested that the terminal galactosyl residue of the glycoconjugates is alpha 2, 3-sialylated in the human conjunctival goblet cells.
Assuntos
Túnica Conjuntiva/citologia , Glicoconjugados/análise , Túnica Conjuntiva/química , Glicoconjugados/química , Histocitoquímica , Humanos , Lectinas/análiseRESUMO
The binding sites of the antibody to cytosolic sialidase on the rat and monkey photoreceptor cells were examined immunohistochemically using the avidin-biotinylated peroxidase method. In the rat photoreceptor cells, the antibody bound diffusely to the inner segment and the outer nuclear layers which are composed chiefly of rod cells. In the monkey photoreceptor cells, the antibody bound to the rod inner segments which were clearly distinct, morphologically, from the cone inner segments. The antibody also bound to the rod cell bodies in the outer nuclear layer. These binding patterns show that the antibody bound preferentially to rod photoreceptor cells. This observation is consistent with previous lectin histochemical findings that sialoglycans are preferentially present on the surfaces of rod photoreceptors, and in the rod-associated interphotoreceptor matrix. Sialidase in rod inner segments may function by balancing with sialyltransferase, also preferentially expressed in rod inner segments, to form sialyl residues on the termini of sugar chains in the rod-associated sialoglycoconjugates.
Assuntos
Citosol/enzimologia , Neuraminidase/metabolismo , Células Fotorreceptoras/enzimologia , Animais , Sítios de Ligação , Técnicas Imunoenzimáticas , Macaca , Ratos , Ratos Wistar , Células Fotorreceptoras Retinianas Bastonetes/enzimologiaRESUMO
The carbohydrate chains of O-linked glycoconjugates in dysplastic retina from a Japanese female infant with Norrie disease were examined by lectin histochemistry. The avidin-biotinylated peroxidase method was used. The retina was highly dysplastic and composed of undifferentiated embryonic tissues containing a number of rosettes of varying sizes. The lumina of the rosettes were stained by peanut agglutinin, which recognizes the Gal beta 1,3GalNAc sequence of O-linked glycans. However, the lumina were not labeled by wheat germ agglutinin, which reacts with sialic acid and/or N-acetylglucosamine. These observations suggest that the O-linked glycoconjugates in the lumina of rosettes were not sialylated in the present case. Their lack of terminal sialic acids may be related to the rosette formation.
Assuntos
Antígenos Glicosídicos Associados a Tumores/metabolismo , Anormalidades do Olho/metabolismo , Glicoconjugados/metabolismo , Deficiência Intelectual/metabolismo , Retina/metabolismo , Displasia Retiniana/metabolismo , Acetilglucosamina/metabolismo , Anormalidades do Olho/genética , Anormalidades do Olho/patologia , Feminino , Histocitoquímica , Humanos , Técnicas Imunoenzimáticas , Lactente , Deficiência Intelectual/genética , Deficiência Intelectual/patologia , Lectinas , Ácido N-Acetilneuramínico/metabolismo , Aglutinina de Amendoim , Polissacarídeos/metabolismo , Retina/patologia , Displasia Retiniana/genética , Displasia Retiniana/patologia , Aglutininas do Germe de TrigoRESUMO
The distribution of alpha 2,3-sialyltransferase (alpha 2,3-ST) mRNA in the rat iris and ciliary body was investigated with in situ hybridization histochemistry. Strong expression of alpha 2,3-ST mRNA was detected in the inner epithelial layer of the ciliary body and weak expression in the iris epithelium. Since the synthesis of sialoglycoconjugates is completed by terminal sialylation by the action of sialyltransferase (ST), the ST-expressed portions are considered to produce sialoglycoconjugates. Hence, the source of the sialoglycoconjugates found in the inner epithelial layer of the ciliary body in previous histochemical studies is the same epithelial cell.
Assuntos
Corpo Ciliar/metabolismo , Iris/metabolismo , RNA Mensageiro/análise , Sialiltransferases/genética , Animais , Sequência de Bases , Epitélio/metabolismo , Dados de Sequência Molecular , Ratos , Ratos Wistar , Distribuição Tecidual , beta-D-Galactosídeo alfa 2-6-SialiltransferaseRESUMO
The binding of amaranthin, specific for Gal beta 1,3 GalNAc and sialic acid Gal beta 1,3 GalNAc sequences, to the human retina was investigated with avidin biotinylated peroxidase. Amaranthin bound to the cone and rod photoreceptors, inner plexiform layer, ganglion cells, and nerve fibers. Since peanut agglutinin, specific for Gal beta 1,3 GalNAc, selectively binds to cones, we conclude that O-glycoside-linked glycoconjugates are present on the surfaces of both cones and rods: Gal beta 1,3 GalNAc and sialic acid Gal beta 1,3 GalNAc are terminal sugars of the glycoconjugates around cones and rods, respectively.
Assuntos
Lectinas/metabolismo , Lectinas de Plantas , Retina/metabolismo , Glicoconjugados/metabolismo , Humanos , Células Fotorreceptoras/metabolismo , Proteínas Inativadoras de Ribossomos , Proteínas Inativadoras de Ribossomos Tipo 1RESUMO
Glycoconjugates, consisting of O- and N-linked types, are present on the surface of photoreceptor cells and in the interphotoreceptor matrix (IPM). This study was undertaken to facilitate understanding of the metabolic features of O-linked sialoglycoconjugates in comparison with those of N-linked glycoconjugates in the rat retina. We examined the developmental change in distribution of Gal beta 1,3GalNAc alpha 2,3-sialyltransferase mRNA in rat retinas using in situ hybridization histochemistry to detect the synthesis period of O-linked sialoglycans. Positive hybridization signal was observed in the ganglion cells throughout the postnatal days (P) examined, whereas strong signals were detected in the photoreceptor inner segments and the inner nuclear layer exclusively at P16 and P18. At P20 and older, weak or sparse signals were detected in these regions. It is likely that O-linked sialoglycoconjugates, which are detected in the mature IPM, are actively synthesized from P16 to P18 in the rat photoreceptor inner segments, suggesting that the O-linked sialoglycoconjugates in the photoreceptor layer may be stable with litter turnover in the mature retina.
Assuntos
Regulação Enzimológica da Expressão Gênica , RNA Mensageiro/biossíntese , Retina/enzimologia , Retina/crescimento & desenvolvimento , Sialiltransferases/biossíntese , Animais , Feminino , Histocitoquímica , Hibridização In Situ , Células Fotorreceptoras/enzimologia , Gravidez , Sondas RNA , Ratos , Ratos Wistar , Retina/citologia , Células Ganglionares da Retina/enzimologia , Sialiltransferases/genética , beta-Galactosídeo alfa-2,3-SialiltransferaseRESUMO
The glycocalyx, present on the surface of the corneal epithelium, contains sialoglycoconjugates. The developmental change in the sialylated residues may be evaluated by examining the expression of the sialyltransferase mRNA. We examined the distribution of Gal beta 1,3GalNAc alpha 2,3-sialyltransferase mRNA in rat corneas during development using in situ hybridization histochemistry to detect the starting point of the synthesis of O-linked sialoglycoconjugates. Eyelid opening occurred between postnatal days 14 (P14) and 16 (P16). In the corneal epithelium, little hybridization signal was observed until P12, whereas distinct hybridization signals were identified at P14 and thereafter. The expression of alpha 2,3-sialyltransferase mRNA is developmentally regulated, based on the programmed time-course of the gene expression, and the corneal epithelium may start to synthesize O-linked sialoglycoconjugates prior to the critical eyelid opening stage.
Assuntos
Córnea/enzimologia , Regulação da Expressão Gênica no Desenvolvimento , Regulação Enzimológica da Expressão Gênica , RNA Mensageiro/metabolismo , Sialiltransferases/metabolismo , Animais , Sequência de Bases , Córnea/crescimento & desenvolvimento , Epitélio/enzimologia , Epitélio/crescimento & desenvolvimento , Feminino , Hibridização In Situ , Masculino , Dados de Sequência Molecular , Oligonucleotídeos/química , Gravidez , Ratos , Ratos Wistar , Sialiltransferases/genética , beta-Galactosídeo alfa-2,3-SialiltransferaseRESUMO
The developmental changes in the binding of Maackia amurensis lectin, specific for sialic acid alpha 2,3 galactose sequence, to the rat retina was investigated using the avidin-biotinylated peroxidase method. The lectin bound to the surfaces of photoreceptor outer segments from postnatal day 16 (P16), whereas it had bound to the other retinal layers from P14. The intense labelings of the outer segments were interspersed with unstained portions, which may correspond to cone photoreceptors. These results confirm that the sialic acid residues on the terminus of carbohydrate chains increase at P16 and mask the beta-galactose residues around rod outer segments.
Assuntos
Fito-Hemaglutininas/metabolismo , Retina/crescimento & desenvolvimento , Retina/metabolismo , Animais , Glicoconjugados/análise , Histocitoquímica , Ratos , Ratos Wistar , Retina/química , Segmento Externo da Célula Bastonete/química , Segmento Externo da Célula Bastonete/crescimento & desenvolvimento , Segmento Externo da Célula Bastonete/metabolismo , Ácidos Siálicos/análiseRESUMO
The binding of amaranthin, specific for the Gal beta 1,3 GalNAc and NeuAc alpha 2,3 Gal beta 1,3 GalNAc sequences, to the photoreceptors of the monkey retina was investigated using the avidin-biotinylated peroxidase method. Amaranthin bound to the surfaces of both cone and rod photoreceptors. This and previous lectin histochemical studies show that O-glycoside-linked glycoconjugates are present on the surfaces of both cones and rods: Gal beta 1,3 GalNAc and NeuAc alpha 2,3 Gal beta 1,3 GalNAc are the terminal sugars of the glycoconjugates around cones and rods, respectively.